The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Diversity of Endophytic Fungi Isolated from Pinus densiflora in Chuncheon city, Gangwon province, Korea

강원도 춘천시의 소나무(Pinus densiflora)에서 분리한 내생균류의 다양성

  • Dae Ho Kim (Tree Pathology and Mycology Laboratory, Kangwon National University) ;
  • Ju Hyun Yong (Tree Pathology and Mycology Laboratory, Kangwon National University) ;
  • Jong Kyu Lee (Tree Pathology and Mycology Laboratory, Kangwon National University)
  • 김대호 (강원대학교 산림과학부 수목병리학 및 균학실험실) ;
  • 용주현 (강원대학교 산림과학부 수목병리학 및 균학실험실) ;
  • 이종규 (강원대학교 산림과학부 수목병리학 및 균학실험실)
  • Received : 2023.12.05
  • Accepted : 2023.12.27
  • Published : 2023.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Red pine (Pinus densiflora) is a dominant tree species in Korean forests. The diversity of endophytic fungi was investigated by isolating fungi from different parts of a red pine tree during the fall and spring seasons. One hundred and fifty-two strains of endophytic fungi were isolated and classified into 42 species belonging to 29 genera. Among these, 74 (49%), 47 (31%) and 31 strains (20%) were isolated from branches, leaves, and stems, respectively. The most frequently isolated species were Daldinia childiae and Biscogniauxia maritima (28.9% and 9.8%, respectively). D. childiae was the dominant endophytic fungus on branches (20 strains) and leaves (19 strains). B. maritima was most frequently isolated from branches. The isolation frequencies on stems of B. maritima (6 strains) and D. childiae (5 strains) were also high. Fifty-seven isolates belonging to 19 species and 17 genera were isolated in the spring, and 95 strains belonging to 35 species and 24 genera were isolated in the fall. D. childiae was the most frequently isolated fungus in both spring and fall (21 and 23 strains, respectively). B. maritima was also frequently isolated in both spring (7 strains) and fall (8 strains).

우리나라 산림에 가장 많이 서식하고 있는 수종인 소나무(Pinus densiflora)의 내생균 다양성을 분석할 목적으로 강원도 춘천에 서식하고 있는 소나무 시료를 봄철과 가을철에 침엽, 가지, 줄기 등 부위별로 구분하여 채집하였다. 채집 시료에서 내생균 152 균주를 분리하여 29속 42종으로 동정하였다. Daldinia childiae가 44균주(28.9%)로 가장 많이 분리된 우점종이었고, Biscogniauxia maritima가 15균주(9.8%)로 분리빈도가 높았다. 부위별로는 가지에서 74균주(49%), 잎에서 47균주(31%), 줄기에서 31균주(20%)로 가지에서 가장 많은 내생균이 분리되었다. 부위별 우점종은 가지와 잎에서는 D. childiae (각각 20균주, 19균주)이었고 가지에서는 B. maritima (7균주)도 자주 분리가 되었다. 잎에서는 B. maritima가 6균주, D. childiae가 5균주로 자주 분리되었다. 채집시기에 따른 소나무 내생균의 다양성은 봄철에는 57균주가 분리되어 17속 19종으로 동정되었고 가을철에는 95균주가 분리되어 24속 35종으로 동정되었다. 자주 분리된 종으로는 봄철과 가을철 모두 공통적으로D. childiae (각각 21균주, 23균주)의 분리빈도가 가장 높았고 Biscogniauxia maritima (7균주, 8균주)도 자주 분리되었다.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A1B03034531)

References

  1. Shin SC, Han HR. Current status on research and management of pine wilt disease in Korea. Seoul: In Proceedings of the International Symposium on Current Status on Research and Management of Pine Wilt Disease; 2006. p. 31-40. 
  2. Kim JB, Kim ES, Lim JH. Topographic and meteorological characteristics of Pinus densiflora dieback areas in Sogwang-Ri, Uljin. Korean J Agric For Meteorol 2017;19:11-2.  https://doi.org/10.5532/KJAFM.2017.19.1.10
  3. Lee CY, Jo BH. Pine, pine forest. Seoul: National Institute of Forest Science; 1999. p. 102-3. 
  4. Esteban L, De Palacios P, Aguado LRL. Abies pinsapo forests in Spain and Morocco threats and conservation. Oryx 2010;44:276-84.  https://doi.org/10.1017/S0030605310000190
  5. Carroll G. Fungal endophytes in stems and leaves from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9.  https://doi.org/10.2307/1943154
  6. Petrini O, Sieber TN, Toti L, Viret O. Ecology, metabolite production, and substrate utilization in endophytic fungi. Nat Toxins 1993;1:185-96.  https://doi.org/10.1002/nt.2620010306
  7. Arnold AE. Understanding the diversity of foliar endophytic fungi progress, challenges, and frontiers. Fungal Biol Rev 2007;21:51-66.  https://doi.org/10.1016/j.fbr.2007.05.003
  8. Dreyfuss MM, Chapela IH. "Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticals" in The discovery of natural products with therapeutic potential. London: Butterworth Heinemann; 1994. p. 49-80. 
  9. Bouton J, Gates R, Belesky D, Owsley M. Yield and persistence of tall fescue in the southeastern coastal plain after removal of its endophyte. Agron J 1993;85:52-5.  https://doi.org/10.2134/agronj1993.00021962008500010011x
  10. Huang WY, Cai Y, Surveswaran S, Hyde KD, Corke H, Sun M. Molecular phylogenetic identification of endophytic fungi isolated from three Artemisia species. Fungal Divers 2009;2009:69-88. 
  11. Clay K. Effects of fungal endophytes on the seed and seedling biology of Lolium perenne and Festuca arundinacea Oecologia 1987;73:358-62.  https://doi.org/10.1007/BF00385251
  12. Eo JK, Kim CK, Eom AH. Fungal endophytes isolated from needle leaves of three coniferous species on Mt. Seodae of Korea. Kor J Mycol 2015;43:133-6.  https://doi.org/10.4489/KJM.2015.43.2.133
  13. Liu JY, Song YC, Zhang Z, Wang L, Guo ZJ, Zou WX, Tan RX. Aspergillus fumigatus CY018, an endophytic fungus in Cynodon dactylon as a versatile producer of new and bioactive metabolites. J Biotechnol 2004;114:279-87.  https://doi.org/10.1016/j.jbiotec.2004.07.008
  14. Zhang HW, Song YC, Tan RX. Biology and chemistry of endophytes. Nat Prod Rep 2006;23:753-71.  https://doi.org/10.1039/b609472b
  15. Hata K, Futai K, Tsuda M. Seasonal and needle age-dependent changes of the endophytic mycobiota in Pinus thunbergii and Pinus densiflora needles. Canad J Bot 1998;76:245-50.  https://doi.org/10.1139/b97-177
  16. Hoff JA, Klopfenstein NB, McDonald GI, Tonn JR, Kim MS, Zambino PJ, Hessburg PF, Rogers JD, Peever TL, Carris LM. Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa). For Pathol 2004;34:255-71.  https://doi.org/10.1111/j.1439-0329.2004.00367.x
  17. Wang Y, Guo LD, Hyde KD. Taxonomic placement of sterile morphotypes of endophytic fungi from Pinus tabulaeformis (Pinaceae) in northeast China based on rDNA sequences. Fungal Divers 2005;20:235-60. 
  18. Wang Y, Guo LD. A comparative study of endophytic fungi in needles, bark, and xylem of Pinus tabulaeformis. Canad J Bot 2007;85:911-7.  https://doi.org/10.1139/B07-084
  19. Sousa JPB, Aguilar-Perez MM, Arnold AE, Rios N, Coley PD, Kursar TA, Cubilla-Rios L. Chemical constituents and their antibacterial activity from the tropical endophytic fungus Diaporthe sp. F2934. J Appl Microbiol 2016;120:1501-8.  https://doi.org/10.1111/jam.13132
  20. Park JH, Gyung JC, Hyang BL, Kyoung MK, Hack SJ, Seon WL, Kyoung SJ, Kwang YC, Jin CK. Griseofulvin from Xylaria sp. strain F0010, an endophytic fungus of Abies holophylla and its antifungal activity against plant pathogenic fungi. J Microbiol Biotechnol 2005;15:112-7. 
  21. Seo ST, Kim KH, Kim MJ, Hong JS, Park JH, Shin SC. Diversity of fungal endophytes from Pinus koraiensis leaves in Korea. Kor J Mycol 2009;37:108-10.  https://doi.org/10.4489/KJM.2009.37.1.108
  22. Kim CK. Biodiversity and seasonal variation of foliar endophytic fungi in conifers. Cheongju: Korea National University of Education; 2013. p. 57-60. 
  23. Kim CK, Eo JK, Eom AH. Diversity of endophytic fungi isolated from leaves of coniferous trees on Mt. Minjuji, Korea. Kor J Mycol 2014;42:174-7.  https://doi.org/10.4489/KJM.2014.42.2.174
  24. Gil YJ, Eo JK, Eom AH. Molecular identification and diversity of endophytic fungi isolated from Pinus densiflora in Boeun, Korea. Kor J Mycol 2009;37:130-3.  https://doi.org/10.4489/KJM.2009.37.2.130
  25. Eo JK, Kim CK, Lee HB, Eom AH. Diversity of endophytic fungi isolated from Pinus densiflora and Larix kaempferi in Mt. Oser, Korea. Kor J Mycol 2013;41:137-41.  https://doi.org/10.4489/KJM.2013.41.3.137
  26. Greenfield M, Pareja R, Ortiza V, Gomez-Jimenez MI, Vega FE, Parsa S. A novel method to scale up fungal endophyte isolations. Biocontrol Sci Technol 2015;25:1208-12.  https://doi.org/10.1080/09583157.2015.1033382
  27. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics in PCR protocols: a guide to methods and applications. New York: Academic Press Inc.; 1990. p. 315-322. 
  28. Voglmayr H, Friebes G, Gardiennet A, Jaklitsch WM. Barrmaelia and Entosordaria in Barrmaeliaceae (fam. nov., Xylariales) and critical notes on Anthostomella-like genera based on multigene phylogenies. Mycol Prog 2018;17:155-77.  https://doi.org/10.1007/s11557-017-1329-6
  29. Parkin EJ, Butlin RK. Within-and between-individual sequence variation among ITS1 copies in the meadow grasshopper Chorthippus parallelus indicates frequent intrachromosomal gene conversion. Mol Biol Evol 2004;21:1595-601.  https://doi.org/10.1093/molbev/msh163
  30. Keylock C. Simpson diversity and the Shannon-Wiener index as special cases of a generalized entropy. Oikos 2005;109:203-7.  https://doi.org/10.1111/j.0030-1299.2005.13735.x
  31. Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021;38:3022-7.  https://doi.org/10.1093/molbev/msab120
  32. Rivera-Orduna FN, Suarez-Sanchez RA, Flores-Bustamante ZR, Gracida-Rodriguez JN, Flores-Cotera LB. Diversity of endophytic fungi of Taxus globosa (Mexican yew). Fungal Divers 2011;47:65-74.  https://doi.org/10.1007/s13225-010-0045-1
  33. Zhao ZZ, Chen HP, Huang Y, Zhang SB, Li ZH, Feng T, Liu JK. Bioactive polyketides and 8, 14-seco-ergosterol from fruiting bodies of the ascomycete Daldinia childiae. Phytochemistry 2017;142:68-75.  https://doi.org/10.1016/j.phytochem.2017.06.020
  34. Choi MS. Diversity and anthrax tolerance of foliar endophytic fungi isolated from ginseng (Panax ginseng C.A. Mey.) in Korea. Cheongju: Korea National University of Education; 2012. 
  35. Lee BH. Biodiversity of endophytic fungi isolated from roots of native orchid in Korea. Cheongju: Korea National University of Education; 2016. 
  36. Oh SY, Yang JH, Woo JJ, Oh SO, Hur JS. Diversity and distribution patterns of endolichenic fungi in Jeju Island, South Korea. Sustainability 2022;12:3769. 
  37. Lim JH, Kim JH, Bae SW. Natural regeneration patten of pine seedlings on the burned forest site in Gosung, Korea. Korean J Agric For Meteorol 2012;14:222-8.  https://doi.org/10.5532/KJAFM.2012.14.4.222
  38. Fisher PJ, Petrini O, Scott HML. The distribution of some fungal and bacterial endophytes in maize (Zea mays L.). New Phytol 1992;122:299-305.  https://doi.org/10.1111/j.1469-8137.1992.tb04234.x
  39. Guo LD, Hyde KD, Liew ECY. Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol 2000;147:617-30. https://doi.org/10.1046/j.1469-8137.2000.00716.x