The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Intestinal Immunomodulatory Effect and Marker Compound of Centella asiatica Extracts-Added Beverage Prototype

병풀 추출물이 첨가된 음료 시제품의 장내 면역조절 효과와 지표물질

  • Yeon Suk Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Hyun Young Shin (Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University) ;
  • Ja Pyeong Koo (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Eun Ji Ha (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Won Bi Jeong (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Mi Yeun Joung (Corporation ChamSunJin Green Juice) ;
  • Kwang-Won Yu (Major in Food and Nutrition, Korea National University of Transportation)
  • 김연숙 (한국교통대학교 식품영양학전공 ) ;
  • 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템 융합전공) ;
  • 구자평 (한국교통대학교 식품영양학전공 ) ;
  • 하은지 (한국교통대학교 식품영양학전공 ) ;
  • 정원비 (한국교통대학교 식품영양학전공 ) ;
  • 정미연 ((주)참선진녹즙 ) ;
  • 유광원 (한국교통대학교 식품영양학전공 )
  • Received : 2023.10.05
  • Accepted : 2023.10.31
  • Published : 2023.12.31
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Abstract

To produce an intestinal immunomodulatory beverage containing Centella asiatica extract (CAE), three types of CAE-added beverage prototypes were prepared, and their immunomodulatory activities and marker compounds were analyzed. As a result of the cytotoxicity assessment, all the beverages did not show significant toxicity compared to the control group. Next, the immunomodulatory activities of the beverage prototype were evaluated using the inflammatory model of IL-1β-induced intestinal epithelial cell line. All the samples significantly reduced the production of IL-6, IL-8, and MCP-1 in a CAE concentration-dependent manner. In addition, CAE-added beverages inhibited NO, IL-6, and IL-12 production in LPS-induced RAW 264.7 cells. When the major triterpenoids, as marker compounds for the production of CAE-added beverages, were analyzed by HPLC-DAD, only asiaticoside was detected beyond the limit of quantification, while madecassoside, madecassic acid, and asiatic acid were not detected. The amounts of asiaticoside in CAE-added beverage prototypes were confirmed in No. 1 (19.39 ㎍/mL), 2 (19.25 ㎍/mL), and 3 (19.98 ㎍/mL). In conclusion, the results of this study suggested that CAE-added beverage prototypes induced immunomodulatory effects in the intestinal inflammatory cell line models and asiaticoside could be used as a marker compound for CAE-added beverage production.

Keywords

Acknowledgement

본 논문은 2023년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과입니다(2021RIS-001; 핵심기술개발사업).

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