한국어병학회지 (Journal of fish pathology)

  • 제36권2호
  • /
  • Pages.415-422
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  • 2023
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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티오아세트아미드(thioacetamide) 복강투여로 인한 무지개송어, Oncorhynchus mykiss의 간장 및 신장 독성 반응 연구

Hepatic and renal toxicity study of rainbow trout, Oncorhynchus mykiss, caused by intraperitoneal administration of thioacetamide (TAA)

  • 허민도 (부경대학교 수산생명의학과) ;
  • 정다혜 (부경대학교 수산생명의학과)
  • Min Do Huh (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University) ;
  • Da Hye Jeong (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University)
  • 투고 : 2023.08.01
  • 심사 : 2023.11.30
  • 발행 : 2023.12.31
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초록

In veterinary medicine for mammals, studies are being conducted to confirm the effects of antioxidants using pathological toxicity model studies, and are also used to confirm the effect of mitigating liver or kidney toxicity of specific substances. It was considered necessary to study such a toxicity model for domestic farmed fish, so thioacetamide (TAA), a toxic substance that causes tissue damage by mitochondrial dysfunction, was injected into rainbow trout (Oncorhynchus mykiss), a major farmed freshwater fish species in Korea. The experiment was conducted with 40 rainbow trout (Oncorhynchus mykiss) weighting 53 ± 0.6 g divided into two groups. Thioacetamide(TAA) 300mg/kg of body weight was intraperitoneally injected into rainbow trout and samples were taken 1, 3, 5, 7 days after peritoneal injection. As a result, in serum biochemical analysis, AST levels related to liver function decreased 3 and 5 days after intraperitoneal injection and increased after 7 days, and ALT levels also increased after 7 days. In addition, creatinine related to renal malfunction increased 3 and 5 days after TAA injection. In histopathological analysis, pericholangitis and local lymphocyte infiltration were observed in the liver from 1 day after intraperitoneal injection of TAA, and hepatic parenchymal cell necrosis was also observed from 3 days after intraperitoneal injection. Hyaline droplet in renal tubular epithelial cell was observed from 1 day after TAA injection, and acute tubular damage such as tubular epithelial cell necrosis appeared from 3 days after TAA injection. Accordingly, it is thought that it will be able to contribute to studies that require a toxicity model.

키워드

과제정보

이 논문(또는 저서)은 부경대학교 자율창의학술연구비(2021년)에 의하여 연구되었음.

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