한국어병학회지 (Journal of fish pathology)

  • 제36권2호
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  • Pages.349-360
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  • 2023
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지
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수인성 아질산 급성 노출에 따른 잉어, Cyprinus carpio의 반수치사농도, 혈액학적 성상 및 혈장성분의 영향

Effects of lethal concentration, hematological parameters and plasma components of common carp, Cyprinus carpio by waterborne acute nitrite exposure

  • 박소원 (선문대학교 수산생명의학과) ;
  • 안수민 (선문대학교 수산생명의학과) ;
  • 조아현 (선문대학교 수산생명의학과) ;
  • 김준환 (제주대학교 수산생명의학과)
  • So-Won Park (Sun Moon University, Department of Aquatic Life and Medical Science) ;
  • Su-Min An (Sun Moon University, Department of Aquatic Life and Medical Science) ;
  • A-Hyun Jo (Sun Moon University, Department of Aquatic Life and Medical Science) ;
  • Jun-Hwan Kim (Department of Aquatic Life Medicine, College of Ocean Sciences, Jeju National University)
  • 투고 : 2023.10.23
  • 심사 : 2023.11.16
  • 발행 : 2023.12.31
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초록

Common carp (Cyprinus carpio) (Weight 23.05 ± 6.98 g, Length 12.65 ± 1.38 cm) were exposed to waterborne nitrite at 0, 50, 100, 200, 400 and 800 mg NO2 -/L for 96 hours. The lethal concentration 50 (LC50) of C. carpio exposed to waterborne nitrite was 398.6 mg NO2 -/L. Hemoglobin, hematocrit and RBC count were significantly decreased by waterborne nitrite exposure. The MCV (mean mean corpuscular volume) (µl), MCH (mean corpuscular hemoglobin) (pg) and MCHC (mean corpuscular hemoglobin concentration) (%) were significantly increased. The inorganic component, plasma calcium, was significantly increased, and the organic components such as plasma cholesterol and total protein were significantly increased showing a similar tendency with calcium. In enzymatic components, the AST and ALT were also significantly increased by nitrite exposure. The results of this study indicate that exposure to nitrite can affect the survival and hematological physiology of C. carpio.

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과제정보

이 성과는 2023년도 과학기술정보통신부의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No. 2022R1C1C1003858).

참고문헌

  1. Ajani, F., Emikpe, B.O. and Adeyemo, O.K.: Histopathological and Enzyme Changes in Clarias gariepinus (Burchell 1822) Exposed to Nitrite at Different Water Temperatures. Nature and Science., 9: 119-124, 2011.
  2. Atwood, H.L., Fontenot, Q.C., Tomasso J.R. and Isely, J.J.: Toxicity of nitrite to Nile tilapia: effect of fish size and environmental chloride. N. Am. J. Aquac., 63: 49-51, 2001. https://doi.org/10.1577/1548-8454(2001)063<0049:TONTNT>2.0.CO;2
  3. Azevedo, M., Souza, M.M. and Freire, C.A.: Reversibility of deleterious effects of the pisciculture byproduct nitrite on cultured Nile tilapia (Oreochromis niloticus). Aquatic Living Resources., 17: 19-23, 2004. https://doi.org/10.1051/alr:2004006
  4. Avilez, I.M., Altran, A.E., Aguiar, L.H. and Moraes, G.: Hematological responses of the Neotropical teleost matrinxa (Brycon cephalus) to environmental nitrite. Com. Biochem. Physiol. C. Toxicol. Pharmacol., 139: 135-139, 2004. https://doi.org/10.1016/j.cca.2004.10.001
  5. Banaee, M., Akhlaghi, M., Soltanian, S., Gholamhosseini, A., Heidarieh, H. and Fereidouni, M.S.: Acute exposure to chlorpyrifos and glyphosate induces changes in hemolymph biochemical parameters in the crayfish, Astacus leptodactylus (Eschscholtz, 1823). Com. Biochem. Physiol. C. Toxicol. Pharmacol., 222: 145-155, 2019. https://doi.org/10.1016/j.cbpc.2019.05.003
  6. Banaee, M., Soltanian, S., Sureda, A., Gholamhosseini, A., Haghi, B.N., Akhlaghi, M. and Derikvandy, A.: Evaluation of single and combined effects of cadmium and micro-plastic particles on biochemical and immunological parameters of common carp (Cyprinus carpio). Chemosphere., 236: 124335, 2019. https://doi.org/10.1016/j.chemosphere.2019.07.066
  7. Brucka-Jastrzebska, E., and Protasowicki, M.: Effects of cadmium and nickel exposure on haematological parameters of common carp, Cyprinus carpio L. Acta. Ichthyol. Piscat., 35: 29-38, 2005. https://doi.org/10.3750/aip2005.35.1.04
  8. Cheng, C.H., Su, Y.L., Ma, H.L., Deng, Y.Q., Feng, J., Chen, X.L. and Guo, Z.X.: Nitrite-induced oxidative stress, histopathology, and transcriptome changes in the mud crab (Scylla paramamosain). Isr. J. Aquac., 71:1626-1637, 2019. https://doi.org/10.46989/001c.20956
  9. Ciji, A. and Akhtar, M.S.: Nitrite implications and its management strategies in aquaculture: A review. Rev. Aquac., 12: 878-908, 2020. https://doi.org/10.1111/raq.12354
  10. Ciji, A., Sahu, N.P., Pal, A.K., Dasgupta, S. and Akhtar, M.S.: Alterations in serum electrolytes, antioxidative enzymes and haematological parameters of Labeo rohita on short-term exposure to sublethal dose of nitrite. Fish. Physiol. Biochemi., 38: 1355-1365, 2012. https://doi.org/10.1007/s10695-012-9623-3
  11. Das, P.C., Ayyappan, S., Jena, J.K. and Das, B.K.: Effect of sub-lethal nitrite toxicity on the hematological parameters of fingerlings of rohu, Labeo rohita(Ham.). Indian journal of fisheries., 51: 287-294, 2004.
  12. de Oliveira, P.R., Bechara, G.H., Denardi, S.E., Pizano, M.A. and Mathias, M.I.C.: Toxicity effect of the acaricide fipronil in semi-engorged females of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae): Preliminary determination of the minimum lethal concentration and LC50. Exp. Parasitol., 127: 418-422, 2011. https://doi.org/10.1016/j.exppara.2010.09.009
  13. de Sousa Miranda, D.H., Maltez, L.C., Santo Campello, M.E., Cordova, J.F.L., Rodrigues, R.V., Sampaio, L.A. and Okamoto, M.H.: Acute toxicity and sublethal effects of nitrite on oxidative stress in early juvenile Brazilian flounder, Paralichthys orbignyanus. Aquac. Res., 53: 1939-1946, 2022. https://doi.org/10.1111/are.15722
  14. Dolezelova, P., Macova, S., Pistekova, V., Svobodova, Z., Bedanova, I. and Voslarova, E.: Nitrite toxicity assessment in Danio rerio and Poecilia reticulata. Acta. Vet. Brno., 80: 309-312, 2011. https://doi.org/10.2754/avb201180030309
  15. dos Santos Silva, M.J., da Costa, F.F.B., Leme, F.P., Takata, R., Costa, D.C., Mattioli, C.C., Luz, R.K. and Miranda-Filho, K.C.: Biological responses of Neotropical freshwater fish Lophiosilurus alexandri exposed to ammonia and nitrite. Sci. Total. Environ., 616: 1566-1575, 2018. https://doi.org/10.1016/j.scitotenv.2017.10.157
  16. Gaim, K., Gebru, G. and Abba, S.: The effect of arsenic on liver tissue of experimental animals (fishes and mice)-a review article. International Journal of Scientific and Research Publications., 5: 1-9, 2015.
  17. Gutierrez, X.A., Kolarevic, J., Takle, H., Baeverfjord, G., Ytteborg, E. and Fyhn Terjesen, B.: Effects of chronic sub-lethal nitrite exposure at high water chloride concentration on Atlantic salmon (Salmo salar, Linnaeus 1758) parr. Aquac. Res., 50: 2687-2697, 2019. https://doi.org/10.1111/are.14226
  18. Hu, Z., Qi, C., Lin, C. and Tang, R.: Nitrite Stress Induces Oxidative Stress and Leads to Muscle Quality Decreased in Wuchang Bream (Megalobrama amblycephala Yih) Juveniles. Water., 14: 160, 2022. https://doi.org/10.3390/w14020160
  19. Jia, R., Liu, B.L., Han, C., Huang, B. and Lei, J.L.: The physiological performance and immune response of juvenile turbot (Scophthalmus maximus) to nitrite exposure. Com. Biochem. Physiol. C. Toxicol. Pharmacol., 181: 40-46, 2016. https://doi.org/10.1016/j.cbpc.2016.01.002
  20. Jeong, J.H., Eun, J.S., Joo, C.H., Jo, A.H., Hong, S.M. and Kim, J.H.: Effects on lethal concentration 50%, hematological parameters and plasma components of mirror carp, Cyprinus carpio nudus exposed to waterborne ammonia. Jouranl of Fish Pathology., 35: 65-75, 2022. http://dx.doi.org/10.7847/jfp.2022.35.1.065
  21. Kanwal, Z., Raza, M.A., Manzoor, F., Riaz, S., Jabeen, G., Fatima, S. and Naseem, S.: A comparative assessment of nanotoxicity induced by metal (silver, nickel) and metal oxide (cobalt, chromium) nanoparticles in Labeo rohita. Nanomaterials., 9: 309, 2019. https://doi.org/10.3390/nano9020309
  22. Kim, J.H., Kang, Y.J. and Lee, K.M.: Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, Epinephelus lanceolatus♂× Epinephelus fuscoguttatus♀. Antioxidants., 11: 545, 2022. https://doi.org/10.3390/antiox11030545
  23. Kir, M., Topuz, H., Sunar, M.C., Topuz, M.: Effect of temperature on acute toxicity of nitrite to meagre, Argyrosomus regius (Asso, 1801). Journal of the World Aquaculture Society., 46: 564-568, 2015. https://doi.org/10.1111/jwas.12214
  24. Kocour Kroupova, H., Valentova, O., Svobodova, Z., Sauer, P. and Machova, J.: Toxic effects of nitrite on freshwater organisms: a review. Rev. Aquac., 10: 525-542, 2018. https://doi.org/10.1111/raq.12184
  25. Kroupova, H., Machova, J., Piackova, V., Blahova, J., Dobsikova, R., Novotny, L. and Svobodova, Z.: Effects of subchronic nitrite exposure on rainbow trout (Oncorhynchus mykiss). Ecotoxicol. Eviron. Saf., 71: 813-820, 2008. https://doi.org/10.1016/j.ecoenv.2008.01.015
  26. Kumar, R. and Banerjee, T.K.: Arsenic induced hematological and biochemical responses in nutritionally important catfish Clarias batrachus (L.). Toxicol. Rep., 3: 148-152, 2016. https://doi.org/10.1016/j.toxrep.2016.01.001
  27. Lefevre, S., Jensen, F.B., Huong, D.T., Wang, T., Phuong, N.T. and Bayley, M.: Haematological and ion regulatory effects of nitrite in the air-breathing snakehead fish Channa striata. Aquat. Toxicol., 118: 48-53, 2012. https://doi.org/10.1016/j.aquatox.2012.03.011
  28. Machat, R., Pojezdal, L., Piackova, V. and Faldyna, M.: Carp edema virus and immune response in carp (Cyprinus carpio): Current knowledge. J. Fish. Dis., 44: 371-378, 2021. https://doi.org/10.1111/jfd.13335
  29. Lek, S., U.t, V.N. and Phuong, N.T.: Effects of nitrite at different temperatures on physiological parameters and growth in clown knifefish (Chitala ornata, Gray 1831). Aquaculture., 521: 735060, 2020. https://doi.org/10.1016/j.aquaculture.2020.735060
  30. Lin, Y., Miao, L.H., Pan, W.J., Huang, X., Dengu, J.M., Zhang, W.X., Ge, X.P., Liu, B., Ren, M.C., Zhou, Q.L., Xie, J., Pan, L.K. and Xi, B.W.:. Effect of nitrite exposure on the antioxidant enzymes and glutathione system in the liver of bighead carp, Aristichthys nobilis. Fish. Shellfish. Immunol., 76: 126-132, 2018. https://doi.org/10.1016/j.fsi.2018.02.015
  31. Madison, B.N. and Wang, Y.S.: Haematological responses of acute nitrite exposure in walleye (Sander vitreus). Aquat. Toxicol., 79: 16-23, 2006. https://doi.org/10.1016/j.aquatox.2006.04.011
  32. Malarvizhi, A., Kavitha, C., Saravanan, M. and Ramesh, M.: Carbamazepine (CBZ) induced enzymatic stress in gill, liver and muscle of a common carp, Cyprinus carpio. J. King. Saud. Univ. Sci., 24: 179-186, 2012. https://doi.org/10.1016/j.jksus.2011.01.001
  33. Molayemraftar, T., Peyghan, R., Jalali, M.R. and Shahriari, A.: Single and combined effects of ammonia and nitrite on common carp, Cyprinus carpio: Toxicity, hematological parameters, antioxidant defenses, acetylcholinesterase, and acid phosphatase activities. Aquaculture., 548: 737676, 2022. https://doi.org/10.1016/j.aquaculture.2021.737676
  34. Rahman, M.M.: Role of common carp (Cyprinus carpio) in aquaculture production systems. Front. Life. Sci., 8: 399-410, 2015. https://doi.org/10.1080/21553769. 2015.1045629
  35. Rashida, Y., Jehu, A., Sallau, B. and Balarebe, M.L.: Sublethal toxicity of glyphosate and propanil on some biochemical parameters in juveniles of freshwater catfish, Clarias gariepinus (Teugels, 1986). Int. J. Fish. Aquat. Stud., 10: 40-45, 2022. https://doi.org/10.33545/26649926.2022.v4.i1b.94
  36. Rodgers, E.M. and De Boeck, G.: Nitrite-induced reductions in heat tolerance are independent of aerobic scope in a freshwater teleost. J. Exp. Biol., 222: jeb212035, 2019. https://doi.org/10.1242/jeb.212035
  37. Sohn, S.G., Lee, Y.S., Kim, K.W., Lee, H.N., Lee, J.Y. and Back, S.J.: Acute Toxicity of Nitrite on Juvenile Banded Catfish(Pseudobagurus fulvidraco). Journal of Fisheries and Marine Sciences Education., 27: 41-48, 2015. http://dx.doi.org/10.13000/JFMSE.2015.27.1.41
  38. Saoud, P., Naamani, S., Ghanawi, J. and Nasser, N.: Effects of acute and chronic nitrite exposure on rabbitfish Siganus rivulatus growth, hematological parameters, and gill histology. J. Aquac. Res. Dev., 5, 2014. https://doi.org/10.4172/2155-9546.1000263
  39. Svobodova, Z., Machova, J., Drastichova, J,. Groch, L., Luskova, V., Poleszczuk G., Velisek, J. and Kroupova, H.: Haematological and biochemical profiles of carp blood following nitrite exposure at different concentrations of chloride. Aquac. Res., 36: 1177-1184, 2005. https://doi.org/10.1111/j.1365-2109.2005.01334.x
  40. Valencia-Castaneda, G., Frias-Espericueta, M.G., VanegasPerez, R.C., Chavez-Sanchez, M.C. and Paez-Osuna, F.:. Physiological changes in the hemolymph of juvenile shrimp Litopenaeus vannamei to sublethal nitrite and nitrate stress in low-salinity waters. Environ. Toxicol. Pharmacol., 80: 103472, 2020. https://doi.org/10.1016/j.etap.2020.103472
  41. Xu, Z., Zhang, H., Guo, M., Fang, D., Mei, J. and Xie, J.: Analysis of Acute Nitrite Exposure on Physiological Stress Response, Oxidative Stress, Gill Tissue Morphology and Immune Response of Large Yellow Croaker (Larimichthys crocea). Animals., 12: 1791, 2022. https://doi.org/10.3390/ani12141791
  42. Yaqoob, S.: A Review of Structure, Origin, Purpose & Impact of Common Carp (Cyprinuscarpio) in India. Ann. Rom. Soc. Cell. Biol., 25: 34-47, 2021.
  43. Zhang, Y., Liang, X.F., He, S. and Li, L.: Effects of long-term low-concentration nitrite exposure and detoxification on growth performance, antioxidant capacities, and immune responses in Chinese perch (Siniperca chuatsi). Aquaculture., 533: 736123, 2021. https://doi.org/10.1016/j.aquaculture.2020.736123
  44. Zhang, H., Fang, D., Mei, J., Xie, J. and Qiu, W.: A Preliminary Study on the Effects of Nitrite Exposure on Hematological Parameters, Oxidative Stress, and Immune-Related Responses in Pearl Gentian Grouper. Fishes., 7: 235, 2022. https://doi.org/10.3390/fishes7050235
  45. Zhang, M., Yin, X., Li, M., Wang, R., Qian, Y. and Hong, M.: Effect of nitrite exposure on haematological status, oxidative stress, immune response and apoptosis in yellow catfish (Pelteobagrus fulvidraco). Com. Biochem. Physiol. C. Toxicol. Pharmacol., 238: 108867, 2020. https://doi.org/10.1016/j.cbpc.2020.108867