한국어병학회지 (Journal of fish pathology)

  • 제36권2호
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  • Pages.277-286
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  • 2023
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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국내 조피볼락(Sebastes sclegelii) 양식장에서 분리한 Streptococcus iniae의 표현형 및 유전형 특성

Pheno- and genotyping of Streptococcus iniae isolated from cultured rockfish, Sebastes schlegelii at Korean coastal sites

  • 김태호 (국립수산과학원 병리연구과) ;
  • 한현자 (국립수산과학원 병리연구과) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 조미영 (국립수산과학원 병리연구과) ;
  • 김수진 (국립수산과학원 병리연구과)
  • Tae-Ho Kim (Pathology Division, National Institute of Fisheries Science) ;
  • Hyun-Ja Han (Pathology Division, National Institute of Fisheries Science) ;
  • Myoung Sug Kim (Pathology Division, National Institute of Fisheries Science) ;
  • Miyoung Cho (Pathology Division, National Institute of Fisheries Science) ;
  • Soo-Jin Kim (Pathology Division, National Institute of Fisheries Science)
  • 투고 : 2023.11.24
  • 심사 : 2023.12.07
  • 발행 : 2023.12.31
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초록

Korean rockfish, Sebastes schlegelii, is a representative bony fish that belongs to the family Scorpaenidae and the order Scorpaeniformes. It has high ecological and economic value and is widely cultivated in many East Asian countries, including South Korea, Japan and China. One of streptococci, Streptococcus iniae, is Gram-positive cocci with a negative reaction for catalase and oxidase. The Korean rockfish shows clinical signs when infected with S. iniae, such as body darkening, bleeding, enlarged kidneys, blurred eyes, abdominal distension, etc., ultimately leading to death. The Korean rockfish causes significant economic losses every year in South Korea due to streptococcosis. In this study, we identified bacteria from the fish using polymerase chain reaction and conducted analyses of hemolytic activity and biochemical tests using API 20 STREP and API ZYM systems. Results of confirming the hemolytic activity (n=4) observed in alpha-type hemolysis (25%), beta-type hemol- ysis (50%), and gamma-type hemolysis (25%) of isolates. The biochemical test results exhibited sig- nificant variation among S. iniae. Additionally, we performed intraperitoneal injection with S. iniae in the fish and analyzed the phylogenetic tree using housekeeping genes of S. iniae, including cpsD, arcC, glnA, groEL, gyrB, mutS, pheT, prkC, rpoB, and tkt, via multilocus sequence typing (MLST). The lethal dose (LD50) showed strong pathogenicity, such as 3.34 × 10 colony-forming unit (CFU)/ml for 23FBStr0601 strain and 7.16 × 10 CFU/ml for 23FBStr0602 strain. 23FBStr0603 strain showed relatively low pathogenicity at 1.73 × 105 CFU/ml. The strains 23FBStr0601 and 23FBStr0602, which showed strong pathogenicity, clustered into one monophyletic group. The 23FBStr0603 strain showed weak pathogenicity and formed a monophyletic group with KCTC 3657.

키워드

과제정보

이 연구는 국립수산과학원 (R2023052, 고효율 어류백신 및 어종별(넙치) 접종 프로그램 개발)의 지원에 의해 진행되었습니다.

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