한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제36권6호
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  • Pages.571-579
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  • 2023
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지
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Efficient Cryopreservation of in vitro Grown Shoot Tips of Pear (Pyrus spp.) by Droplet-vitrification

  • Jae-Young Song (National Agrobiodiversity Center, NAS, RDA) ;
  • Jinjoo Bae (National Agrobiodiversity Center, NAS, RDA) ;
  • Ji-Won, Han (National Agrobiodiversity Center, NAS, RDA) ;
  • Ho Cheol Ko (National Agrobiodiversity Center, NAS, RDA) ;
  • Ho-sun Lee (National Agrobiodiversity Center, NAS, RDA) ;
  • Sung-Hee Nam (National Agrobiodiversity Center, NAS, RDA) ;
  • Jung-RoLee (National Agrobiodiversity Center, NAS, RDA) ;
  • Byeong Hyeon Yun (Fruit Research Division, National Institute of Horticultural & Herbal Science) ;
  • Keumsun Kim (Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA)) ;
  • Kyungho Won (Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA)) ;
  • Il Sheob Shin (Pear Research Institute, National Institute of Horticultural and Herbal Science, Rural Development Administration (NIHHS-RDA))
  • 투고 : 2023.10.09
  • 심사 : 2023.11.14
  • 발행 : 2023.12.01
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초록

In this study, cryopreservation by droplet-vitrification was applied to pear (Pyrus spp.) germplasm. We focused on the development and assessment of various strategies for the selection of suitable tissue, osmoprotection, and dehydration. We also evaluated post-thaw recovery of cryopreserved explants by droplet-vitrification. Preferentially, we tested the effects of preculture and loading treatments to determine which tissues were more suitable, either the apical shoot tips or the axillary buds. Apical shoot tips showed the better regrowth rate than in vitro axillary buds. The most effective techniques for cryopreservation were as follows. Shoots from in vitro seedlings which had been cultured for about 5-6 weeks were cold-hardened at 4℃ for one week, excised shoot tips were precultured on liquid MS medium including 0.3 M sucrose for 31 hours and 0.7 M sucrose for 17 hours, osmoprotected in loading solution (LS) for 40 min, and then cryoprotected in dehydration solution (PVS3) for 90 min. In addition, we found that regrowth rates of explants on regrowth medium after exposure to liquid nitrogen (LN) were higher than those on MS medium. Results indicated that the highest regrowth percentage was 95.6% for 'Bartlett' cultivar and 68.9% for 'BaeYun No.3' cultivar. Consequently, apical shoot tips of two pear cultivars, 'Bartlett' (P. communis) and 'BaeYun No.3' (P. pyrifolia), were successfully cryopreserved by droplet-vitrification. Results of this study show that the enhanced droplet-vitrification method described in the present study could be used as an effective means for long-term storage of pear genetic resources.

키워드

과제정보

This study was carried out with the support of "Development and application of cryopreservation protocol for pear and freesia germplasm (Project No.PJ016811)", National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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