Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Effect of Pulsed Electromagnetic Field Stimulation on TNF-α/IFN-γ induced inflammatory response in human skin keratinocytes HaCaT Cell to reduce inflammatory factors

인간 피부각질세포 HaCaT Cell에서 TNF-α/IFN-γ로 유도된 염증 반응에 대한 펄스형 전자기장(PEMF) 자극의 염증 인자 완화 효과

  • Jun Young Kim (Department of Biomedical Engineering, Yonsei University) ;
  • Chan Ho Park (Department of Anatomy, Yonsei University Wonju College of Medicine) ;
  • Chang Soon Park (Department of Biomedical Engineering, Yonsei University) ;
  • Yong Heum Lee (Department of Biomedical Engineering, Yonsei University)
  • 김준영 (연세대학교 의공학과) ;
  • 박찬호 (연세대학교 원주의과대학 해부학교실) ;
  • 박창순 (연세대학교 의공학과) ;
  • 이용흠 (연세대학교 의공학과)
  • Received : 2023.11.26
  • Accepted : 2023.12.11
  • Published : 2023.12.31
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Abstract

The purpose of this study was to observe cell death in human keratinocytes stimulated against the infectious cytokines TNF-α and IFN-γ, and to observe the expression of Phospho-NF-κB due to phosphorylation of IkB to confirm the mechanism of inhibiting the expression of inflammatory cytokines. As a result of cell viability analysis, differences in PEMF stimulation time were observed little by little after 1 hour, 3 hours, 6 hours, 12 hours, 24 hours, and 48 hours, but there was no statistical significance according to PEMF stimulation time for each time (p>0.05). No significant difference was observed in the total amount of NF-κB present in the cytoplasm and nucleus, but a significant decrease in the expression of phosphorylated NF-κB was observed in the group exposed to PEMF stimulation for 24 hours (*p<0.05). The expression of IL-1β was observed in all inflammation-induced groups, and the concentration of IL-1β compared to α-Tubulin expression was reduced by about 54% in the PEMF-stimulated group for 24 hours compared to the control group (***p<0.001). As a result of the study, it is shown that PEMF stimulation does not negatively affect HaCaT cells from 0 to 48 hours and can inhibit the expression of inflammatory cytokines by inhibiting the pathway of NF-κB.

Keywords

Acknowledgement

본 연구는 2023년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업(2022RIS-005)과 연세대학교 미래융합연구원(ICONS)의 지원을 통해 수행되었습니다.

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