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Effect of Water Adulteration on the Rheology and Antibacterial Activities of Honey

  • ANIDIOBU, Vincent Okechukwu (Department of Food Science and Technology, Faculty of Agriculture, Forestry and Wildlife Resources Management, University of Calabar)
  • Received : 2022.08.28
  • Accepted : 2022.11.25
  • Published : 2022.11.30

Abstract

Honey was diluted with different percentages of water and was analysed rheologically at room temperature of 27℃. The rheological profiles of pure and impure honey samples were measured at low shear rates (0.01-4.16s-1). This work developed a structural kinetic model, which correlated well with the rheological data. The new model was used to categorise honey samples using their average molecular weights as one of the distinctive properties. Also, the kinetics order in the new model predicts the number of active components in the "honey" undergoing deformation. Honey produced third order kinetics to depict the monomers, oligomers and water content in honey. Pure honey exhibits peculiar non-Newtonian rheological behaviour. The behaviour of water is Newtonian. Dilution of honey with different percentages of water turns the resulting fluid Newtonian from 10% dilution with water. This study analysed the antibacterial activities of honey and serially adulterated samples against Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial analyses of honey were conducted using Kirby Bauer's well diffusion method. The results indicated that pure honey exhibited a zone of inhibition against both organisms. Also, the diameter of the zone of inhibition decreased with increasing dilution of honey, suggesting a correlation with the rheological method.

Keywords

References

  1. Adebiyi, F.M., Akpan, I., Obiajunwa, E.I., & Olaniyi, H.B. (2004). Chemical/physical characterisation of Nigerian honey. Pakistan Journal of Nutrition, 3(5):278-281. https://doi.org/10.3923/pjn.2004.278.281
  2. Ajibola, A, Chamunorwa, J.P., & Erlwanger, K.H. (2012). Nutraceutical values of natural honey and its contribution to human health and wealth. Nutrition & Metabolism, 61(9):1-12.
  3. Anidiobu, V.O, (2014). Rheological Modeling of the Effect of Sucrose Adulteration on Nigerian Honey, Nigerian Food Journal, 32(2): 103-112. https://doi.org/10.1016/s0189-7241(15)30125-9
  4. Anidiobu, V.O. (2021). Rheological determination of molecular weight of honey adulterated with high fructose corn syrup. Journal of Apicultural Research, 60(1):1-8. https://www.tandfonline.com/doi/full/10.1080/00218839.2021.1874708
  5. Bogdanov, S. (2004). Physicochemical methods for the characterization of unifloral honey: A review. Apidologie, 35(1):4-17. https://doi.org/10.1051/apido:2004047
  6. Cheesbrough, M. (2010). District Laboratory Practice in Tropical Countries, Part 2 2nd Edition., Cambridge, UK: Cambridge University Press. pp 45-62.
  7. Chute, R.K, Deogade. N.G., & Kawale, M. (2010). Antibacterial activity of Indian honey against clinical isolates. Asiatic Journal of Biotechnology Resources, 1: 35-38.
  8. Clinical and Laboratory Standards Institute (CLSI) (2020). Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100 (ISBN 978-1-68440-066-9 [Print]; ISBN 978-1-68440-067-6 [Electronic]). Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087 USA.
  9. Everstine, K., Spink, J. & Kennedy, S. (2013). Economically motivated adulteration (EMA) of food: common characteristics of EMA incidents. Journal of Food Protection, 76 (4): 723-735. https://doi.org/10.4315/0362-028X.JFP-12-399
  10. Hilmi, M., Bradbear, N., & Mejia, D. (2011). Beekeeping and sustainable livelihood, Food and Agricultural Organisation Diversification Booklet, 1:43-63.
  11. Kurzbeck, S., Oster, F., Munstedt, H., Nguyen, T.Q., & Gensler, R. (1999). Rheological properties of two polypropylenes with different molecular structures. Journal of Rheology, 359(43): 1-11.
  12. Launay, B., Doublier, J. R., & Cuvelier, G. (1986). Flow properties of aqueous solutions and dispersions of polysaccharides. In J. R. Mitchell & D. A. Ledward (Eds.), Functional properties of food macromolecules, London, UK: Elsevier Applied Science. pp.1-78.
  13. Lazaridou, A., Biliaderis C.G., Bacandritsos, N., & Sabatini, A.G. (2004). Composition, thermal and rheological behaviour of selected Greek Honeys. Journal of Food Engineering, 64(1): 9-21. https://doi.org/10.1016/j.jfoodeng.2003.09.007
  14. Morrison, F.A. (1999). Using the solver Add-in in Microsoft Excel.www.google.com/rheological modelling.
  15. Ndip, R. N.,Takang, E.A.M., Echakachi, C.M., Malongue, A. , Akoachere, J.T.K. ,Ndip,L.M., & Luma, H.N. (2007). Invitro antimicrobial activity of selected honeys on clinical isolates of Helicobacter pylori. African Health Science, 7(4):228-232.
  16. Nguyen, Q. D., Jensen, C. T. B., & Kristensen, P. G. (1998). Experimental and modelling studies of the flow properties of maise and waxy starch pastes, Chemical Engineering Journal, 70:165-171. https://doi.org/10.1016/S0923-0467(98)00081-5
  17. Nwalor, J.U, Babalola, F.U & Anidiobu, V.O. (2014, July 15). Rheological Characterisation of Honey: Application as an Index of Quality. In P. Scales & P. Ranganathan (Eds.), Abstract Proceedings of the 6th Pacific Rim Conference on Rheology, Melbourne, Australia. pp 211-212.
  18. Saissy, J. M., Guignar, B., Pats, B., Guiavarch, M, & Rouvier, B.(1995). Pulmonary edema after hydrogen peroxide irrigation of a war wound. Intensive care Medical Journal, 21(3): 287-288.
  19. Triantafillopoulos, N. (1988). Measurement of Fluid Rheology and Interpretation of Rheograms second edition. Kaltech Scientific, Inc. 22425 Heslip Drive Novi, Michigan 48375 USA.
  20. White, J.W. Jr. (1992). Quality evaluation of honey: Role of HMF and diastase assays. American Bee Journal, 132 (11 & 12): 737-743, 792-794.