The Journal of the Convergence on Culture Technology (문화기술의 융합)

The International Promotion Agency of Culture Technology (국제문화기술진흥원)
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Anti-Biofilm Activity of Origanum Vulgare Supercritical Fluid Extracts and Cosmetic Active Ingredients Development

오레가노 초임계추출물의 황색포도상구균 바이오필름 형성 억제능을 이용한 기능성 화장품 소재의 개발

  • 박신성 (을지대학교 미용화장품과학과) ;
  • 이광원 (을지대학교 시니어헬스케어학과) ;
  • 박수인 (을지대학교 미용화장품과학과) ;
  • 신문삼 (을지대학교 미용화장품과학과)
  • Received : 2022.08.14
  • Accepted : 2022.10.10
  • Published : 2022.11.30
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Abstract

In this study, oregano was extracted by supercritical extraction and hydrothermal extraction method. In vitro experiments such as antimicrobial and antioxidant activity test were performed. As a result of the disc diffusion method, only the supercritical extracts formed a clear zone. The MIC for S. aureus was found only in the supercritical fluid extracts and it was 1000 ㎍/mL. The hydrothermal extract's MIC is 125 ㎍/mL for C. acnes. Through biofilm inhibition assay, we found that the supercritical fluid oregano extracts inhibit the biofilm of S. aureus by more than 70% even at low concentrations of 125 ㎍/mL. On the other hand, the antioxidant ability of the hydrothermal extract was better than that of the supercritical fluid extracts. Furthermore, we tried to make a skincare ingredient for atopic dermatitis by utilizing the S. aureus biofilm inhibitory ability of oregano supercritical fluid extracts. Liposome was used to overcome the low solubility of the oregano supercritical fluid extracts and increase stability.

본 연구에서는 오레가노를 초임계유체추출법과 열수추출법으로 추출하여 항균, 항산화 in vitro 실험을 수행하였다. 항균실험인 Disc diffusion method 결과, 초임계추출물에 대해서만 clear zone이 나타났다. S. aureus에 대한MIC는 초임계추출물에서만 관찰되었으며, 1000 ㎍/mL로 확인되었다. 열수추출물은 C. acnes에 대한 MIC가 125 ㎍/mL로 여드름균에 대한 항균력이 뛰어났다. 나아가 우리는 Biofilm inhibition assay를 통해 오레가노 초임계추출물이 125 ㎍/mL의 저농도에서도 S. aureus의 바이오필름을 70%이상 억제한다는 것을 발견하였다. DPPH radical 소거능, ABTS+ radical 소거능, SOD 유사활성능과 총 폴리페놀 함량 측정에서 열수추출물의 항산화력이 초임계추출물보다 우수하게 측정되었다. 나아가, 앞서 확인된 오레가노 초임계추출물의 S. aureus 바이오필름 억제능을 활용하여 아토피 피부에 적합한 소재를 개발하고자 하였다. 오레가노 초임계추출물의 낮은 용해도를 극복하고 안정성을 증가시키기 위하여 리포좀을 이용하였다.

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References

  1. R.V. Houdt and C. W. Michiels, "Biofilm formation and the food industry, a focus on the bacterial outer surface," Journal of Applied Microbiology, Vol. 109, No. 4, pp. 1117-1131, October 2010. DOI:　https://doi.org/10.1111/j.1365-2672.2010.04756.x
  2. R. Donlan, "Biofilms: Microbial life on surfaces", Emerg Infect Dis, Vol. 8, No. 9, pp. 881-890, September 2002. DOI: https://doi.org/10.3201/eid0809.020063
  3. T.B. Rasmussen, and M. Givskov, "Quorum -sensing inhibitors as antipathogenic drugs," International Journal of Medical Microbiology, Vol. 296, No. 23, pp. 149-161, February 2006. DOI: https://doi.org/10.1016/j.ijmm.2006.02.005
  4. J. W. Costerton, P. S. Stewart, and E. P. Greenberg, "Bacterial biofilms: a common cause of persistent infections," Science, Vol. 284, No. 5418, pp. 1318-1322, May 1999. DOI: https://doi.org/10.1126/science.284.5418.1318
  5. J. L. Del Pozo, "Biofilm-related disease," Expert Rev Anti Infect Ther, Vol. 16, No. 1, pp. 51-65 December 2017. DOI: https://doi.org/10.1080/14787210.2018.1417036
  6. S. H. Song, B. W. Hwang, S. Son, and N. G. Kang, "A Study on Inhibition of Bacterial Membrane Formation in Biofilm formed by Acne Bacteria in Valine through Property Analysis," J ournal of the Society of Cosmetic Scientists of Korea, Vol. 47, No. 2 pp. 163-170, June 2021. DOI: https://doi.org/10.15230/SCSK.2021.47.2.163
  7. J. A. Geoghegan, A. D. Irvine, and T. J. Foster, "Staphylococcus aureus and Atopic Dermatitis: A Complex and Evolving Relationship," Trends in microbiology, Vol. 26, No. 6, pp. 484-497, December 2017. DOI: https://doi.org/10.1016/j.tim.2017.11.008
  8. H. B. Allen, N. D. Vaze, C. Choi, T. Hailu, B. H. Tulbert, C. A. Cusack, and S. G. Joshi, "The presence and impact of biofilm-producing staphy lococci in atopic dermatitis," JAMA dermatology, Vol. 150, No. 3, pp. 260-265, March, 2014. DOI: https://doi.org/10.1001/jamadermatol.2013.8627
  9. H. Sakkas, and C. Papadopoulou, "Antimicrobial Activity of Basil, Oregano, and Thyme Essential Oils," Journal of microbiology and biotechnology, Vol. 27, No. 3, pp. 429-438, March 2017. DOI: https://doi.org/10.4014/jmb.1608.08024
  10. N. Leyva-Lopez, E. P. Gutierrez-Grijalva, G. Vazquez-Olivo, and J. B. Heredia, "Essential Oils of Oregano: Biological Activity beyond Their Antimicrobial Properties," Molecules, Vol. 22, No. 6, pp. 989-1013, June 2017. DOI: https://doi.org/10.3390/molecules22060989
  11. M. Walczak, M. Michalska-Sionkowska, D. Olkiewicz, P. Tarnawska, and O. Warzynska, "Potential of Carvacrol and Thymol in Reducing Biofilm Formation on Technical Surfaces," Molecules, Vol. 26, No. 9, pp. 2723-2735, May 2021. DOI: https://doi.org/10.3390/molecules26092723
  12. S. I. Park, S. H. Heo, J. Lee, and M. S. Shin, "Extraction of Active Compounds from Angelica gigas using Supercritical Carbon Dioxide and its Physiological Activity," Journal of Convergence for Information Technology, Vol. 11, No. 6, pp. 206-212, June 2021. DOI: https://doi.org/10.22156/CS4SMB.2021.11.06.206
  13. K. W. Lee, S. Park, S. I. Park, and M. S. Shin, "Cosmetic Efficacy of Supercritical Cannabis sativa Seed Extracts and Enhancement of Skin Permeation," Journal of the convergence on culture technology, Vol. 7, No.4, pp. 683-691, November, 2021. DOI: https://doi.org/10.17703/JCCT.2021.7.4.683
  14. S. H. Park, and M. H. Kim, "Physical Property and Stability of Liposome Prepared from Egg Yolk Phospholipids at Various Storage Conditions," Korean Society for Food Science of Animal Resources, Vol. 28, No. 5, pp. 549-554. December, 2008. DOI: https://doi.org/10.5851/kosfa.2008.28.5.549
  15. J. Lee, S. I. Park, S. H. Heo, M. Kim, and M. S. Shin, "Enhancing the Moisturizing Ability of the Skin Softener using Nanoemulsion Based on Phospholipid Liposome," International Journal of Advanced Culture Technology, Vol. 8, No. 1, pp. 236-242, March 2020. DOI: https://doi.org/10.17703/IJACT.2020.8.1.236
  16. M. O. Ko, H. J. Kang, J. H. Hwang, and K. W. Yang, "Screening of the antibacterial effects by ethanol extracts from natural plant in Jeju against Propionibacterium acnes," Journal of Society of Cosmetic Scientists of Korea, Vol. 44, No. 1, pp. 59-66, March 2018. DOI: https://doi.org/10.15230/SCSK.2018.44.1.59
  17. P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R.H. Yolke, Manual of clinical microbiology, Mosby Year Book, 1995.
  18. G. A. O'Toole, "Microtiter dish biofilm formation assay," J ournal of visualized experiments, No. 47, 2437, January 2011. DOI: https://doi.org/10.15230/10.3791/2437
  19. M. S. Blois, "Antioxidant determinations by the use of a stable free radical," Nature, Vol. 181, No. 4617 pp. 1199-1200,, April 1958. DOI: https://doi.org/10.1038/1811199a0
  20. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Riceevans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay," Free Radical Biology & Medicine, Vol. 26, No. 9-10, pp. 1231-1237, May 1999. DOI: https://doi.org/10.1016/s0891-5849(98)00315-3
  21. S. Marklund, G. Marklund, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase," European Journal of Biochemistry, Vol. 47, No. 3, pp. 469-474, September 1974. DOI: https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  22. S. I. Park, A. R. Kim, S. H. Kim, G. M. An, M. G. Kim, and M. S. Shin, "Antioxidant, Antiwrinkle and Antimicrobial Effects of Coffee Grounds Extract from Dutch Coffee," Journal of Oil & Applied Science, Vol. 35, No. 4, pp. 1038-1047, December 2018. DOI: https://doi.org/10.12925/jkocs.2018.35.4.1038
  23. J. J. BIEME, "Antimicrobial Susceptibility Testing by the Kirby-Bauer Disc Diffusion Method," Annals of Clinical and Laboratory Science, Vol. 3, No. 2, pp.135-140, March 1973.
  24. I. Wiegand, K. Hilpert, and R. Hancock, "Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances," Nature Protocol, Vol. 3, No. 2, pp. 163-175, February 2008. DOI: https://doi.org/10.1038/nprot.2007.521
  25. P. A. Uwineza, and A. Waskiewicz, "Recent Advances in Supercritical Fluid Extraction of Natural Bioactive Compounds from Natural Plant Materials," Molecules, Vol. 25, No. 17, 3847, September 2020. DOI: https://doi.org/10.3390/molecules25173847
  26. A. K. Lim, J. O. Kim, M. J. Jung, H. K. Jung, J. H. Hong, and D. I. Kim. "Functional biological activity of hot water and ethanol extracts from Taraxaci Herba," Journal of the Korean Society of Food Science and Nutrition, Vol. 37, No. 10, pp. 1231-1237, October 2008. DOI : https://doi.org/10.3746/jkfn.2008.37.10.1231
  27. M. J. Choi, and H. I. Maibach, "Role of ceramides in barrier function of healthy and diseased skin," American Journal of Clinical Dermatology, Vol. 6, No. 4, pp. 215-223, January 2005. DOI: https://doi.org/10.2165/00128071-200506040-00002