Animal Bioscience

  • 제35권12호
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  • Pages.1904-1910
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  • 2022
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effects of intracerebroventricular injection of corticotrophin releasing factor on the gene expression of ghrelin and corticotrophin releasing factor receptors in broiler chickens

  • Cai, Yuanli (College of Life Science, Qilu Normal University) ;
  • Song, Zhigang (Department of Animal Science, Shandong Agricultural University)
  • 투고 : 2022.01.29
  • 심사 : 2022.05.18
  • 발행 : 2022.12.01
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초록

Objective: This study aimed to investigate the effects of corticotropin-releasing factor (CRF) on the feed intake of broiler chickens and explore its influencing mechanism. Methods: The study included two trials. In trial 1, 32 male broiler chickens (Arbor Acres, Gallus gallus domesticus) were given ventricle buried tubes, and they were allowed to recover for 3 days. At 8:00 AM, intracerebroventricular (ICV) injection with CRF or normal saline was performed in 10-day-old broiler chickens, which were divided into the 5, 10, and 20 ㎍ and control (normal saline) groups according to the dose of CRF injection. In trial 2, chickens were divided into the 10 ㎍ and control group (physiological saline) to repeat trial 1. Results: Results of trial 1 showed that the cumulative amount of feed intake in the 10 or 20 ㎍ groups was considerably lower than that of the control group after ICV injection with CRF. The lowest amount of feed intake was obtained with the addition of 10 ㎍ of CRF. In trial 2, the expression of ghrelin in the hypothalamus injected with 10 ㎍ of CRF increased significantly, but the expression of ghrelin in various sections of the small intestine considerably decreased. The expression of CRF receptor subtypes 1 (CRFR1) in the hypothalamus and some parts of the small intestine remarkably increased, and the expression of CRF receptor subtypes 2 (CRFR2) increased only in the duodenum, whereas the expression of growth hormone secretagogue receptor (GHSR-1α) in the jejunum and ileum increased considerably after ICV injection of 10 ㎍ of CRF. Conclusion: The CRF at 10 ㎍ increased ghrelin expression in the hypothalamus and CRFR1 expression in the small intestine, and this phenomenon was related to the suppressed feed intake of broiler chickens.

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과제정보

This work was supported by the National Key R&D Program of China (2021YFD1300404) and Shandong Province Agricultural Industry Technology (SDAIT-11-08).

참고문헌

  1. Gillies G, Grossman A. The CRFs and their control: chemistry, physiology and clinical implications. Clin Endocrinol Metab 1985;14:821-43. https://doi.org/10.1016/s0300-595x(85)80079-7
  2. Richard D, Lin Q, Timofeeva E. The corticotropin-releasing factor family of peptides and CRF receptors: their roles in the regulation of energy balance. Eur J Pharmacol 2002;440: 189-97. https://doi.org/10.1016/s0014-2999(02)01428-0
  3. Dunn AJ, Berridge CW. Physiological and behavioral responses to corticotropin-releasing factor administration: is CRF a mediator of anxiety or stress responses? Brain Res Rev 1990;15:71-100. https://doi.org/10.1016/0165-0173(90)90012-d
  4. Cline MA, Kuo AY, Smith ML, et al. Differential feed intake responses to central corticotrophin releasing factor in lines of chickens divergently selected for low or high body weight. Comp Biochem Physiol A Mol Integr Physiol 2009;152:130-4. https://doi.org/10.1016/j.cbpa.2008.09.008
  5. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999;402:656-60. https://doi.org/10.1038/45230
  6. Wren AM, Small CJ, Ward HL, et al. The novel hypothalamic peptide ghrelin stimulates food intake and growth hormone secretion. Endocrinology 2000;141:4325-8. https://doi.org/10.1210/endo.141.11.7873
  7. Saito ES, Kaiya H, Tachibana T, et al. Inhibitory effect of ghrelin on food intake is mediated by the corticotropinreleasing factor system in neonatal chicks. Regul Pept 2005; 125:201-8. https://doi.org/10.1016/j.regpep.2004.09.003
  8. Davis JL, Masuoka DT, Gerbrandt LK, Cherkin A. Autoradiographic distribution of L-proline in chicks after intracerebral injection. Physiol Behav 1979;22:693-5. https://doi.org/10.1016/0031-9384(79)90233-6
  9. Furuse M, Ando R, Bungo T, et al. Intracerebroventricular injection of orexins does not stimulate food intake in neonatal chicks. Br Poult Sci 1999;40:698-700. https://doi.org/10.1080/00071669987115
  10. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods (San Diego, Calif.) 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  11. Luo X, Kiss A, Makara G, Lolait SJ, Aguilera G. Stress-specific regulation of corticotropin releasing hormone receptor expression in the paraventricular and supraoptic nuclei of the hypothalamus in the rat. J Neuroendocrinol 1994;6:689-96. https://doi.org/10.1111/j.1365-2826.1994.tb00636.x
  12. Blalock JE. A molecular basis for bidirectional communication between the immune and neuroendocrine systems. Physiol Rev 1989;69:1-32. https://doi.org/10.1152/physrev.1989.69.1.1
  13. Morley JE, Levine AS. Corticotrophin releasing factor, grooming and ingestive behavior. Life Sci 1982;31:1459-64. https://doi.org/10.1016/0024-3205(82)90007-8
  14. Rivest S, Richard D. Involvement of corticotropin-releasing factor in the anorexia induced by exercise. Brain Res Bull 1990;25:169-72. https://doi.org/10.1016/0361-9230(90)90270-a
  15. Glowa JR, Barrett JE, Russell J, Gold PW. Effects of corticotropin releasing hormone on appetitive behaviors. Peptides 1992;13:609-21. https://doi.org/10.1016/0196-9781(92)90097-m
  16. Denbow DM, Snapir N, Furuse M. Inhibition of food intake by CRF in chickens. Physiol Behav 1999; 66:645-9. https://doi.org/10.1016/s0031-9384(98)00340-0
  17. Stengel A, Tache Y. Corticotropin-releasing factor signaling and visceral response to stress. Exp Biol Med (Maywood) 2010;235:1168-78. https://doi.org/10.1258/ebm.2010.009347
  18. Brown MR, Fisher LA, Spiess J, Rivier C, Rivier J, Vale W. Corticotropin-releasing factor: actions on the sympathetic nervous system and metabolism. Endocrinology 1982;111:928-31. https://doi.org/10.1210/endo-111-3-928
  19. Phillips RJ, Powley TL. Gastric volume rather than nutrient content inhibits food intake. Am J Physiol 1996;271:R766-9. https://doi.org/10.1152/ajpregu.1996.271.3.R766
  20. Hussain SS, Bloom SR. The regulation of food intake by the gut-brain axis: implications for obesity. Int J Obes (Lond) 2013;37:625-33. https://doi.org/10.1038/ijo.2012.93
  21. Muller TD, Nogueiras R, Andermann ML, et al. Ghrelin. Mol Metab 2015;4:437-60. https://doi.org/10.1016/j.molmet.2015.03.005
  22. Banks WA, Tschop M, Robinson SM, Heiman ML. Extent and direction of ghrelin transport across the blood-brain barrier is determined by its unique primary structure. J Pharmacol Exp Ther 2002;302:822-7. https://doi.org/10.1124/jpet.102.034827
  23. Saito ES, Kaiya H, Takagi T, et al. Chicken ghrelin and growth hormone-releasing peptide-2 inhibit food intake of neonatal chicks. Eur J Pharmacol 2002;453:75-9. https://doi.org/10.1016/s0014-2999(02)02393-2
  24. Chen LL, Jiang QY, Kaiya H, et al. Central administration of chicken ghrelin inhibits food intake in adult chickens. 9th International Symposium on Avian Endocrinology; 2008 July 11-15: Leuven, Belgium. Abstract 091,75.
  25. Papotti M, Ghe C, Cassoni P, et al. Growth hormone secretagogue binding sites in peripheral human tissues. J Clin Endocrinol Metab 2000;85:3803-7. https://doi.org/10.1210/jcem.85.10.6846
  26. van der Lely AJ, Tschop M, Heiman ML, Ghigo E. Biological, physiological, pathophysiological, and pharmacological aspects of ghrelin. Endocr Rev 2004;25:426-57. https://doi.org/10.1210/er.2002-0029
  27. Zhang W, Yu G, Huang Y, et al. Expression of ghrelin and GHSR-1a in mammary glands of dairy goat during the lactation and the effects of gherlin on regulation of mammary function in vitro. Mol Cell Endocrinol 2013;370:20-31. https://doi.org/10.1016/j.mce.2013.02.008
  28. Shousha S, Nakahara K, Kojima M, et al. Different effects of peripheral and central ghrelin on regulation of food intake in the Japanese quail. Gen Comp Endocrinol 2005;141:178-83. https://doi.org/10.1016/j.ygcen.2004.12.021
  29. Kageyama K, Akimoto K, Yamagata S, et al. Dexamethasone stimulates the expression of ghrelin and its receptor in rat hypothalamic 4B cells. Regul Pept 2012;174:12-7. https://doi.org/10.1016/j.regpep.2011.11.003
  30. Yin Y, Li Y, Zhang W. The growth hormone secretagogue receptor: its intracellular signaling and regulation. Int J Mol Sci 2014;15:4837-55. https://doi.org/10.3390/ijms15034837
  31. Kitazawa T, Kaiya H, Taneike T. Contractile effects of ghrelinrelated peptides on the chicken gastrointestinal tract in vitro. Peptides 2007;28:617-24. https://doi.org/10.1016/j.peptides.2006.10.012
  32. Stengel A, Tache Y. CRF and urocortin peptides as modulators of energy balance and feeding behavior during stress. Front Neurosci 2014;8:52. https://doi.org/10.3389/fnins.2014.00052
  33. Fekete EM, Zorrilla EP. Physiology, pharmacology, and therapeutic relevance of urocortins in mammals: ancient CRF paralogs. Front Neuroendocrinol 2007;28:1-27. https://doi.org/10.1016/j.yfrne.2006.09.002
  34. Webster EL, Lewis DB, Torpy DJ, Zachman EK, Rice KC, Chrousos GP. In vivo and in vitro characterization of antalarmin, a nonpeptide corticotropin-releasing hormone (CRH) receptor antagonist: suppression of pituitary ACTH release and peripheral inflammation. Endocrinology 1996;137:5747-50. https://doi.org/10.1210/endo.137.12.8940412
  35. Hotta M, Shibasaki T, Arai K, Demura H. Corticotropinreleasing factor receptor type 1 mediates emotional stressinduced inhibition of food intake and behavioral changes in rats. Brain Res 1999;823:221-5. https://doi.org/10.1016/s0006-8993(99)01177-4
  36. Prater CM, Harris BN, Carr JA. Tectal CRFR1 receptors modulate food intake and feeding behavior in the South African clawed frog Xenopus laevis. Horm Behav 2018;105:86-94. https://doi.org/10.1016/j.yhbeh.2018.07.013
  37. Perrin MH, Vale WW. Corticotropin releasing factor receptors and their ligand family. Ann NY Acad Sci 1999;885:312-28. https://doi.org/10.1111/j.1749-6632.1999.tb08687.x