Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Synthesis of ginsenoside Rb1-imprinted magnetic polymer nanoparticles for the extraction and cellular delivery of therapeutic ginsenosides

  • Liu, Kai-Hsi (Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital) ;
  • Lin, Hung-Yin (Department of Chemical and Materials Engineering, National University of Kaohsiung) ;
  • Thomas, James L. (Department of Physics and Astronomy, University of New Mexico) ;
  • Shih, Yuan-Pin (Department of Chemical and Materials Engineering, National University of Kaohsiung) ;
  • Yang, Zhuan-Yi (Department of Chemical Engineering, I-Shou University) ;
  • Chen, Jen-Tsung (Department of Life Sciences, National University of Kaohsiung) ;
  • Lee, Mei-Hwa (Department of Materials Science and Engineering, I-Shou University)
  • Received : 2021.04.19
  • Accepted : 2022.01.26
  • Published : 2022.09.01
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Abstract

Background: Panax ginseng (ginseng) is a traditional medicine that is reported to have cardioprotective effects; ginsenosides are the major bioactive compounds in the ginseng root. Methods: Magnetic molecularly imprinted polymer (MMIP) nanoparticles might be useful for both the extraction of the targeted (imprinted) molecules, and for the delivery of those molecules to cells. In this work, plant growth regulators were used to enhance the adventitious rooting of ginseng root callus; imprinted polymeric particles were synthesized for the extraction of ginsenoside Rb1 from root extracts, and then employed for subsequent particle-mediated delivery to cardiomyocytes to mitigate hypoxia/reoxygenation injury. Results: These synthesized composite nanoparticles were first characterized by their specific surface area, adsorption capacity, and magnetization, and then used for the extraction of ginsenoside Rb1 from a crude extract of ginseng roots. The ginsenoside-loaded MMIPs were then shown to have protective effects on mitochondrial membrane potential and cellular viability for H9c2 cells treated with CoCl2 to mimic hypoxia injury. The protective effect of the ginsenosides was assessed by staining with JC-1 dye to monitor the mitochondrial membrane potential. Conclusion: MMIPs can play a dual role in both the extraction and cellular delivery of therapeutic ginsenosides.

Keywords

Acknowledgement

The authors would like to appreciate the Ministry of Science and Technology of ROC under Contract nos.: MOST 106-2221-E-214-038, MOST 107-2221-E-214-017, MOST 108-2923-I-390-001-MY3, MOST 109-2314-B-390-001-MY3, MOST 110-2221-E-214-012 and the Zuoying Branch of Kaohsiung Armed Forces General Hospital under Contract no. KAFGH-ZY_A_111003.

References

  1. Neri M, Riezzo I, Pascale N, Pomara C, Turillazzi E. Ischemia/reperfusion injury following acute myocardial infarction: a critical issue for clinicians and forensic pathologists. Mediat Inflamm 2017;2017:7018393.
  2. Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012;380(9859):2095-128. https://doi.org/10.1016/S0140-6736(12)61728-0
  3. Kurian GA, Rajagopal R, Vedantham S, Rajesh M. The role of oxidative stress in myocardial ischemia and reperfusion injury and remodeling: revisited. Oxid Med Cell Longev 2016;2016:1656450.
  4. Wang Z, Su G, Zhang Z, Dong H, Wang Y, Zhao H, et al. 25-Hydroxyl-protopanaxatriol protects against H(2)O(2)-induced H9c2 cardiomyocytes injury via PI3K/Akt pathway and apoptotic protein down-regulation. Biomedicine & pharmacotherapy - Biomedecine & pharmacotherapie 2018;99:33-42. https://doi.org/10.1016/j.biopha.2018.01.039
  5. Christensen LP. Ginsenosides: chemistry, biosynthesis, analysis, and potential health effects. Adv Food Nutr Res 2008;55:1-99. https://doi.org/10.1016/S1043-4526(08)00401-4
  6. Leung KW, Wong AS-T. Pharmacology of ginsenosides: a literature review. Chin Med 2010;5(1):20. https://doi.org/10.1186/1749-8546-5-20
  7. Cho J, Park W, Lee S, Ahn W, Lee Y. Ginsenoside-Rb1 from Panax ginseng C.A. Meyer activates estrogen receptor-alpha and -beta, independent of ligand binding. J Clin Endocrinol Metab 2004;89(7):3510-5. https://doi.org/10.1210/jc.2003-031823
  8. Hou Y-L, Tsai Y-H, Lin Y-H, Chao JCJ. Ginseng extract and ginsenoside Rb1 attenuate carbon tetrachloride-induced liver fibrosis in rats. BMC Compl Alternative Med 2014;14(1):415. https://doi.org/10.1186/1472-6882-14-415
  9. Liu X, Chen L, Liu M, Zhang H, Huang S, Xiong Y, et al. Ginsenoside Rb1 and Rd remarkably inhibited the hepatic uptake of ophiopogonin D in shenmai injection mediated by OATPs/oatps. Front Pharmacol 2018;9:957. https://doi.org/10.3389/fphar.2018.00957
  10. Li F, Wu Z, Sui X. Biotransformation of ginsenoside Rb1 with wild Cordyceps sinensis and Ascomycota sp. and its antihyperlipidemic effects on the dietinduced cholesterol of zebrafish. J Food Biochem 2020;44(6):e13192.
  11. Zhou W, Chai H, Lin PH, Lumsden AB, Yao Q, Chen C. Ginsenoside Rb1 blocks homocysteine-induced endothelial dysfunction in porcine coronary arteries. J Vasc Surg 2005;41(5):861-8. https://doi.org/10.1016/j.jvs.2005.01.054
  12. Wang X, Chai H, Yao Q, Chen C. Molecular mechanisms of HIV protease inhibitor-induced endothelial dysfunction. JAIDS Journal of Acquired Immune Deficiency Syndromes 2007;44(5):493-9. https://doi.org/10.1097/QAI.0b013e3180322542
  13. Lee SE, Park YS. Korean Red Ginseng water extract inhibits COX-2 expression by suppressing p38 in acrolein-treated human endothelial cells. Journal of Ginseng Research 2014;38(1):34-9. https://doi.org/10.1016/j.jgr.2013.11.004
  14. Zhang B, Matsuda S, Tanaka J, Tateishi N, Maeda N, Wen T-C, et al. Ginsenoside Rb1 prevents image navigation disability, cortical infarction, and thalamic degeneration in rats with focal cerebral ischemia. J Stroke Cerebrovasc Dis 1998;7(1):1-9. https://doi.org/10.1016/S1052-3057(98)80015-3
  15. Fujita K, Hakuba N, Hata R, Morizane I, Yoshida T, Shudou M, et al. Ginsenoside Rb1 protects against damage to the spiral ganglion cells after cochlear ischemia. Neurosci Lett 2007;415(2):113-7. https://doi.org/10.1016/j.neulet.2007.01.005
  16. Yuan Q-L, Yang C-X, Xu P, Gao X-Q, Deng L, Chen P, et al. Neuroprotective effects of ginsenoside Rb1 on transient cerebral ischemia in rats. Brain Res 2007;1167:1-12. https://doi.org/10.1016/j.brainres.2007.06.024
  17. Sakanaka M, Zhu P, Zhang B, Wen T-C, Cao F, Ma Y-J, et al. Intravenous infusion of dihydroginsenoside Rb1 prevents compressive spinal cord injury and ischemic brain damage through upregulation of VEGF and Bcl-XL. J Neurotrauma 2007;24(6):1037-54. https://doi.org/10.1089/neu.2006.0182
  18. Ai Q, Sun G, Luo Y, Dong X, Hu R, Meng X, et al. Ginsenoside Rb1 prevents hypoxia-reoxygenation-induced apoptosis in H9c2 cardiomyocytes via an estrogen receptor-dependent crosstalk among the Akt, JNK, and ERK 1/2 pathways using a label-free quantitative proteomics analysis. RSC Adv 2015;5(33):26346-63. https://doi.org/10.1039/C5RA02432C
  19. Zhang H, Wang X, Ma Y, Shi Y. The effect of ginsenoside RB1, diazoxide, and 5- hydroxydecanoate on hypoxia-reoxygenation injury of H9C2 cardiomyocytes. Evid Based Complement Alternat Med 2019;2019:6046405.
  20. Lee M-H, Thomas JL, Wang H-Y, Chang C-C, Lin C-C, Lin H-Y. Extraction of resveratrol from polygonum cuspidatum with magnetic orcinol-imprinted poly(ethylene-co-vinyl alcohol) composite particles and their in vitro suppression of human osteogenic sarcoma (HOS) cell line. J Mater Chem 2012;22(47):24644-51. https://doi.org/10.1039/c2jm34244h
  21. Lee M-H, Thomas JL, Liao C-L, Jurcevic S, Crnogorac-Jurcevic T, Lin H-Y. Epitope recognition of peptide-imprinted polymers for Regenerating protein 1 (REG1). Separ Purif Technol 2018;192:213-9. https://doi.org/10.1016/j.seppur.2017.09.071
  22. Lee M-H, Thomas JL, Ho M-H, Yuan C, Lin H-Y. Synthesis of magnetic molecularly imprinted poly (ethylene-co-vinyl alcohol) nanoparticles and their uses in the extraction and sensing of target molecules in urine. ACS Appl Mater Interfaces 2010;2(6):1729-36. https://doi.org/10.1021/am100227r
  23. Lee M-H, Thomas JL, Chen Y-C, Wang H-Y, Lin H-Y. Hydrolysis of magnetic amylase-imprinted poly (ethylene-co-vinyl alcohol) composite nanoparticles. ACS Appl Mater Interfaces 2012;4(2):916-21. https://doi.org/10.1021/am201576y
  24. Chen SL, Yang CT, Yang ZL, Guo RX, Meng JL, Cui Y, et al. Hydrogen sulphide protects H9c2 cells against chemical hypoxia-induced injury. Clin Exp Pharmacol Physiol 2010;37(3):316-21. https://doi.org/10.1111/j.1440-1681.2009.05289.x
  25. Shi Y-N, Zhang X-Q, Hu Z-Y, Zhang C-J, Liao D-F, Huang H-L, et al. Genistein protects h9c2 cardiomyocytes against chemical hypoxia-induced injury via inhibition of apoptosis. Pharmacology 2019;103(5-6):282-90. https://doi.org/10.1159/000497061
  26. Skoog F, Miller C, editors. Chemical regulation of growth and organ formation in plant tissues cultured. Vitro Symp Soc Exp Biol; 1957.
  27. Liu K-H, Lin H-Y, Thomas JL, Shih Y-P, Chen J-T, Lee M-H. Extraction of ginsenosides from the Panax ginseng callus using magnetic molecularly imprinted polymer composite nanoparticles. Ind Crop Prod 2021;163:113291-9. https://doi.org/10.1016/j.indcrop.2021.113291
  28. Duncan DB. Multiple range and multiple F tests. Biometrics 1955;11(1):1-42. https://doi.org/10.2307/3001478