Journal of Microbiology and Biotechnology

  • 제32권10호
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  • Pages.1307-1314
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  • 2022
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Prevalence and Characteristics of Salmonella spp. Isolated from Raw Chicken Meat in the Republic of Korea

  • Koh, Youngho (Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Bae, Yunyoung (Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Lee, Yu-Si (Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety) ;
  • Kang, Dong-Hyun (Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute for Agricultural and Life Science, Seoul National University) ;
  • Kim, Soon Han (Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
  • 투고 : 2022.07.15
  • 심사 : 2022.09.05
  • 발행 : 2022.10.28
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초록

In this study, we sought to investigate the various characteristics of Salmonella spp. isolated from raw chicken meats available in Korean markets. The data collected, such as food source of isolation, sampling information, serotype, virulence, and genetic profile including sequence type, were registered in the database for further comparative analysis of the strains isolated from the traceback investigation samples. To characterize serotype, virulence and gene sequences, we examined 113 domestically distributed chicken meat samples for contamination with Salmonella spp. Phylogenetic analysis was conducted on 24 strains (21.2%) of Salmonella isolated from 113 commercially available chicken meats and by-products, using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Serotyping of the isolated Salmonella spp. revealed S. Enteritidis in 11 strains (45.8%), S. Virchow in 6 strains (25%), S. Montevideo in 2 strains (8.3%), S. Bsilla in 2 strains (8.3%), S. Bareilly in 1 strain (4.2%), S. Dessau in 1 strain (4.2%), and S. Albany in 1 strain (4.2%). The genetic correlation indicated that 24 isolated strains were classified into 18 clusters with a genetic similarity of 64.4-100% between them. Eleven isolated S. Enteritidis strains were classified into 9 genotypes with a sequence identity of 74.4%, whereas the most distantly related S. Virchow was divided into five genotypes with 85.9% identity. Here, the MLST analysis indicated that the major Sequence Type (ST) of the Salmonella spp. isolated from domestic chicken sold in Chungcheong Province belongs to the ST 11 and 16, which differs from the genotype of Salmonella isolated from imported chicken. The differential sequence characteristics can be a genetic marker for identifying causative bacteria for epidemiological investigations of food poisoning.

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과제정보

This research was supported by a grant (18161MFDS033) from the Ministry of Food and Drug Safety in 2018.

참고문헌

  1. Lim TH, Lee HJ, Kim MS, Kim BY, Yang SY, Song CS. 2010. Evaluation of efficacy of bacteriophage CJo07 against Salmonella enteritidis infection in the SPF chicks. Korean J. Poult. Sci. 37: 283-287. https://doi.org/10.5536/KJPS.2010.37.3.283
  2. Hong SH, Park NY, JO HJ, Ro EY, Ko YM, Na YJ, et al. 2015. Risk ranking determination of combination of foodborne pathogens and livestock or livestock products. J. Food Hyg. Saf. 30: 1-12. https://doi.org/10.13103/JFHS.2015.30.1.1
  3. Kim SH, Lee YS, Joo IS, Kwak HS, Chung GT, Kim SH. 2018. Rapid detection for Salmonella spp. by ultrafast real-time PCR assay. J. Food Hyg. Saf. 33: 50-57. https://doi.org/10.13103/JFHS.2018.33.1.50
  4. Ministry of Food and Drug Safety (MFDS): www.foodsafetykorea.go.kr. 2022.
  5. KMA Weather Data Service, Open MET Data Portal, Korea Meteorological Administration, Republic of Korea (https://data.kma.go.kr)
  6. Moon HJ, Lim JG, Yoon KS. 2016. Comparative study of change in Salmonella Enteritidis and Salmonella Typhimurium populations in Egg white and yolk. J. Food Hyg. Safety 31: 342-348. https://doi.org/10.13103/JFHS.2016.31.5.342
  7. Na DH, Hong YH, Yoon MY, Park EJ, Lim TH, Jang JH, et al. 2013. Prevalence of Salmonella species isolated from old hen delivery trucks in Korea and application of disinfectant for the reduction of Salmonella contamination. Korean J. Poult. Sci. 40: 11-16. https://doi.org/10.5536/KJPS.2013.40.1.011
  8. Kweon OG, Jin SK, Kim GO, Lee CI, Jeong KH, Kim JY. 2014. Characterization of Salmonella spp. clinical isolates in Gyeongsangbuk-do province, 2012 to 2013, Ann. Clin. Microbiol. 17: 50-56. https://doi.org/10.5145/ACM.2014.17.2.50
  9. Lee HJ. 2001. Salmonellosis. Korean J. Clin. Microbiol. 4: 5-10.
  10. Yang B, Qu D, Zhang X, Shen J, Cui S, Shi Y, et al. 2010. Prevalence and characterization of Salmonella serovars in retail meats of marketplace in Shaanxi, China. Int. J. Food Microbiol. 141: 63-72. https://doi.org/10.1016/j.ijfoodmicro.2010.04.015
  11. Ha DY, Cha HG, Han KS, Jang EH, Park HY, Bae MJ, et al. 2018. Analysis of virulence gene profiles of Salmonella spp. and Enterococcus faecalis isolated from the freshly slaughtered poultry meats produced in Gyeong-Nam province. Korean J. Vet. Serv. 41: 157-163.
  12. Wang X, Jothikumar N, Griffiths MW. 2004. Enrichment and DNA extraction protocols for the simultaneous detection of Salmonella and Listeria monocytogenes in raw sausage meat with multiplex real-time PCR. J. Food Prot. 67: 189-192. https://doi.org/10.4315/0362-028X-67.1.189
  13. Shin WS, Kim YS, Lee JS, Kim MH. 2009. Analysis of Salmonella species from eggs using immunoliposomes and comparison with a commercial test kit. Korean J. Food Sci. Ani. Resour. 29: 533-538. https://doi.org/10.5851/kosfa.2009.29.4.533
  14. Kim GY, Yang GM, Park SB, Kim YH, Lee KJ, Son JY, et al. 2011. Rapid detection kit got Salmonella Typhimurium. J. Biosyst. Eng. 36: 140-146. https://doi.org/10.5307/JBE.2011.36.2.140
  15. Lee JB, Yoon JW. 2018. Hazard analysis of Salmonella contamination in the broiler and duck farms and their slaughtering processing. Safe Food 13: 3-10.
  16. Bugarel M, Granier SA, Weill FX, Fach P, Brisabois A. 2011. A multiplex real-time PCR assay targeting virulence and resistance genes in Salmonella enterica serotype Typhimurium. BMC Microbiol. 11: 151. https://doi.org/10.1186/1471-2180-11-151
  17. Seo KH, Valentin-Bon IE, Brackett RE, Holt PS. 2004. Rapid, specific detection of Salmonella Enteritidis in pooled eggs by Real-Time PCR. J. Food Prot. 67: 864-869. https://doi.org/10.4315/0362-028X-67.5.864
  18. Kim SH, Kim SH, Lee SW, Kang YH, Lee BK. 2005. Rapid serological identification for monophasic Salmonella serovars with a hin gene-specific polymerase chain reaction. J. Bacteriol. Virol. 35: 291-297.
  19. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731-2739. https://doi.org/10.1093/molbev/msr121
  20. Pilar DG, Clavijo V, LEO' N M, Tafur MA, Gonzales S, Hume M, et al. 2012. Prevalence of Salmonella on retail broiler chicken meat carcasses in Colombia. J. Food Prot. 75: 1134-1138. https://doi.org/10.4315/0362-028X.JFP-11-513
  21. Rodriguez JM, Rondon IS, Verjan N. 2015. Serotypes of Salmonella in broiler carcasses marketed at Ibague, Colombia. Brazilian J. Poultry Sci. 17: 545-552. https://doi.org/10.1590/1516-635X1704545-552
  22. Khan AS, Georges1 K, Rahaman S, Abdela W, Adesiyun AA. 2018. Prevalence and serotypes of Salmonella spp. on chickens sold at retail outlets in Trinidad. PLoS One 13: e0202108. https://doi.org/10.1371/journal.pone.0202108
  23. Thorns CJ. 2000. Bacterial food-borne zoonoses. Rev. Sci. Tech. 19: 226-239. https://doi.org/10.20506/rst.19.1.1219
  24. Wilson IG. 2002. Salmonella and Campylobacter contamination of raw retail chickens from different producers: a six year survey. Epidemiol. Infect. 129: 635-645. https://doi.org/10.1017/S0950268802007665
  25. Kang MS, Lee SJ, Shin YS. 2015. Prevalence and antimicrobial resistance of Salmonella isolated in poultry farms. Korean J. Vet. Serv. 38: 95-100. https://doi.org/10.7853/kjvs.2015.38.2.95
  26. Lee HW, Hong CH, Jung BY. 2007. Characteristics of Salmonella spp isolated from poultry carcasses. Kor. J. Vet. Serv. 30:339-351.
  27. Kim SR, Nam HM, Jang GC, Kim AR, Kang MS, Chae MH, et al. 2011. Antimicrobial resistance in Salmonella isolates from food animals and raw meats in Korea during 2010. Kor. J. Vet. Publ. Health 35: 246-254.
  28. Jung SC, Song SW, Kim SI, Jung ME, Kim KH, Lee JY, et al. 2007. Antimicrobial susceptibility of Salmonella spp. isolated from carcasses in slaughterhouse. Kor. J. Vet. Publ. Health 31: 51-56.
  29. Yang HY, Lee SM, Park EJ, Kim JH, Lee JG. 2009. Analysis of antimicrobial resistance and PFGE patterns of Salmonella spp. isolated from chickens at slaughterhouse in Incheon area. Korean J. Vet. Serv. 32: 325-334.
  30. Lim SY, Ryu SR. 2000. Prevalence of Salmonella Enterotoxin gene(stn) among clinical strains isolated in Korea and regulation of stn expression. Kor. J. Appl. Microbiol. Biotechnol. 28: 316-321.
  31. Galan JE, Ginocchio C, Costeas P. 1992. Molecular and functional characterization of Salmonella the invasion gene invA: homology of invA to members of a new protein family. J. Bacteriol. 174: 4338-4349. https://doi.org/10.1128/jb.174.13.4338-4349.1992
  32. Ziemer CJ, Steadham SR. 2003. Evaluation of the specificity of Salmonella PCR primers using various intestinal bacterial species. Lett. Appl. Microbiol. 37: 463-469. https://doi.org/10.1046/j.1472-765X.2003.01430.x
  33. Jeon MH, Kim TJ, Jang GS, Kang GI, Kim GH, Kim GS, et al. 1999. Specific detection of Salmonella serogroup D1 by Polymerase Chain Reaction (PCR) for sefA gene. Korean J. Vet. Res. 39: 523-530.
  34. Lee WW, Lee SM, Lee GR, Lee DS, Park HK. 2009. Identification of Salmonella Enteritidis and S. Typhimurium by multiplex polymerase chain reaction. Korean J. Vet. Serv. 32: 147-153.
  35. Swamy SC, Barnhart HM, Lee MD, Dreesen DW. 1996. Virulence determinants invA and spvC in Salmonellae isolated from poultry products, wastewater, and human sources. Appl. Environ. Microbiol. 62: 3768-3771 https://doi.org/10.1128/aem.62.10.3768-3771.1996
  36. Oliveira SD, Rodenbusch CR, Michae GB, Cardoso MI, Canal CW, Brandelli A. 2003. Detection of virulence genes in Salmonella Enteritidis isolated from different sources. Braz. J. Microbiol. 34: 123-124. https://doi.org/10.1590/S1517-83822003000500042
  37. Woodward MJ, Kirwan SES. 1996. Detection of Salmonella Enteritidis in eggs by the polymerase chain reaction. Vet. Rec. 138: 411-413. https://doi.org/10.1136/vr.138.17.411
  38. Araque M. 2009. Nontyphoid Salmonella gastroenteritis in pediatric patients from urban areas in the city of Merida, Venezuela. J. Infect. Dev. Ctries 3: 28-34. https://doi.org/10.3855/jidc.102
  39. Chaudhary JH, Nayak JB, Brahmbhatt MN, Makwana PP. 2015. Virulence genes detection of Salmonella serovars isolated from pork and slaughterhouse environment in Ahmedabad, Gujarat. Vet. World 8: 121-124. https://doi.org/10.14202/vetworld.2015.121-124
  40. Soto SM, Rodriguez I, Rodicio MR, Vila J, Mendoza MC. 2006. Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles. J. Med. Microbiol. 55: 365-373. https://doi.org/10.1099/jmm.0.46257-0
  41. Almeida F, Silva PD, Medeiros MIC, Rodrigues DDP, Moreira CG, Allard MW, et al. 2017. Multilocus sequence typing of Salmonella Typhimurium reveals the presence of the highly invasive ST313 in Brazil. Infect. Genet. Evol. 51: 41-44. https://doi.org/10.1016/j.meegid.2017.03.009
  42. Amanda Ap. Seribelli AA, Silva P, Cruz1 MF, Almeida F, Frazao MR, et al. 2021. Insights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics, Gut Pathog. 13: 27. https://doi.org/10.1186/s13099-021-00423-7
  43. Campioni F, Vilela FP, Cao G, Kastanis G, Rodrigues RP, Costa RG, et al. 2022. Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades. Sci. Rep. 12: 10555. https://doi.org/10.1038/s41598-022-14492-4