Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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Cytological, histochemical, and ultrastructural study of human foetal liver of various gestation with future implications in segmental resection: an anatomical perspective

  • Arpan Haldar (Department of Anatomy, All India Institute of Medical Sciences) ;
  • Manisha Rajanand Gaikwad (Department of Anatomy, All India Institute of Medical Sciences) ;
  • Apurba Patra (Department of Anatomy, All India Institute of Medical Sciences) ;
  • Soumya C. Bhattacharya (Department of Anatomy, ESI-PGIMSR)
  • Received : 2021.07.22
  • Accepted : 2021.09.24
  • Published : 2022.03.31
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Abstract

The liver is the largest gland of the gastrointestinal tract having both exocrine and endocrine functions. Developmentally it arises as a ventral outgrowth from the gut endoderm during 3rd week of intrauterine life. The foetal liver is very important because of its synthetic and hemopoietic potential. The present work aimed to see the detailed histogenesis and development of the foetal liver by cytological, immunohistochemical and ultrastructural study. The liver tissue of nine aborted foetuses of various gestational age were studied. For cytology: special stains like Masson trichrome, periodic acid Schiff and reticulin were used, immunohistochemical staining was performed with triple antibodies (c-myc, Ki-67 and Ber-H2), and for ultrastructure: aluminium mounted specimens were coated with gold and argon gas and observed under scanning electron microscopy (EM). Cytology and immunohistochemistry showed the development of duct patterns and hemopoietic patterns in all stages of fetogenesis. The ductal plate was marked by the layer of dark brown staining cells at the edge of two portal tracts. The haemopoietic cells with sinusoids and aggregation of hepatocytes were observed in the early weeks of gestation. EM showed tree-like branching of a portal canal depicting hepatic segmentation of foetal liver. The organizational changes in lobular pattern, duct pattern, and microstructure of liver during fetogenesis are very crucial to achieve the adult morphology in feature. Histogenesis of the foetal liver follows a multistep process depending upon the gestational age, any deviation from normalcy may lead to structural and functional abnormality later in life.

Keywords

Acknowledgement

Authors thank EM Facility, SAIF, Department of Anatomy, All India Institute of Medical Sciences, New Delhi and IHC Lab, Department of Anatomy (IHC) and Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Bhubaneswar.

References

  1. Michalopoulos GK. Liver regeneration. J Cell Physiol 2007;213:286-300. 
  2. Sadler TW. Langman's medical embryology. 7th ed. Philadelphia: Lippincott Williams & Wilkins; 2005. p. 198-201. 
  3. Gil MN, Choi DR, Yu KS, Jeong JH, Bak DH, Kim DK, Lee NS, Lee JH, Jeong YG, Na CS, Na DS, Ryu KH, Han SY. Rhus verniciflua Stokes attenuates cholestatic liver cirrhosis-induced interstitial fibrosis via Smad3 down-regulation and Smad7 upregulation. Anat Cell Biol 2016;49:189-98. 
  4. Ajmani ML. Practical embalming. In: Ajmani ML, editor. Text Book Principles of Embalming Techniques in Autopsied Body. New Delhi: Jaypee Brothers Publishers; 1993. p.131-4. 
  5. Naik KS, Lokanadham S, Gurushanthaiah M. Different gestational age related histogenesis of human foetal liver. Int J Anat Res 2020;8:7408-11. 
  6. Terada T, Nakanuma Y, Ohta G. Glandular elements around the intrahepatic bile ducts in man; their morphology and distribution in normal livers. Liver 1987;7:1-8. 
  7. Kieusseian A, Brunet de la Grange P, Burlen-Defranoux O, Godin I, Cumano A. Immature hematopoietic stem cells undergo maturation in the fetal liver. Development 2012;139:3521-30. 
  8. Jaiswal A, Sinha DN, Singh AK. A study on histology of fetal liver. Natl J Clin Anat 2015;4:26-9. 
  9. Zorn AM. Liver development. In: Harvard Stem Cell Institute, editor. StemBook. Cambridge: Harvard Stem Cell Institute; 2008. 
  10. Moore KL, Persaud TVN, Torchia MG. The developing human: clinically oriented embryology. 8th ed. Philadelphia: Elsevier Saunders; 2008. p. 211-42. 
  11. Naito M, Hasegawa G, Takahashi K. Development, differentiation, and maturation of Kupffer cells. Microsc Res Tech 1997;39:350-64. 
  12. Lipp W. [The early structural development of the liver parenchyma in man]. Z Mikrosk Anat Forsch 1952;59:161-86. German. 
  13. Severn CB. A morphological study of the development of the human liver. II. Establishment of liver parenchyma, extrahepatic ducts and associated venous channels. Am J Anat 1972;133:85-107. 
  14. Villeneuve J, Pelluard-Nehme F, Combe C, Carles D, Chaponnier C, Ripoche J, Balabaud C, Bioulac-Sage P, Lepreux S. Immunohistochemical study of the phenotypic change of the mesenchymal cells during portal tract maturation in normal and fibrous (ductal plate malformation) fetal liver. Comp Hepatol 2009;8:5. 
  15. Zamboni L. Electron microscopic studies of blood embryogenesis in humans. II. The hemopoietic activity in the fetal liver. J Ultrastruct Res 1965;12:525-41. 
  16. Hamilton WJ, Boyd JD, Mossman HW. Human embryology: prenatal development of form and function. 4th ed. London: Macmillan; 1978. p. 339-48. 
  17. Slayton WB, Juul SE, Calhoun DA, Li Y, Braylan RC, Christensen RD. Hematopoiesis in the liver and marrow of human fetuses at 5 to 16 weeks postconception: quantitative assessment of macrophage and neutrophil populations. Pediatr Res 1998;43:774-82. 
  18. Couvelard A, Scoazec JY, Dauge MC, Bringuier AF, Potet F, Feldmann G. Structural and functional differentiation of sinusoidal endothelial cells during liver organogenesis in humans. Blood 1996;87:4568-80. 
  19. Emura I, Sekiya M, Ohnishi Y. Two types of immature erythrocytic series in the human fetal liver. Arch Histol Jpn 1983;46:631-43. 
  20. Miranda RN, Omurtag K, Castellani WJ, De las Casas LE, Quintanilla NM, Kaabipour E. Myelopoiesis in the liver of stillborns with evidence of intrauterine infection. Arch Pathol Lab Med 2006;130:1786-91. 
  21. Balis JU, Chan A, Conen PE. Electron microscopy study of the developing human liver. Tijdschr Gastroenterol 1964;7:133-47. 
  22. Potter EL, Craig JM. Weights standards for organs for early human fetuses. In: Potter EL, Craig JM, editors. Pathology of the Fetus and the Infant. 3rd ed. Chicago: Year Book Medical Publishers; 1975. p.15-24. 
  23. Notenboom RG, de Boer PA, Moorman AF, Lamers WH. The establishment of the hepatic architecture is a prerequisite for the development of a lobular pattern of gene expression. Development 1996;122:321-32. 
  24. Payushina OV. Hematopoietic microenvironment in the fetal liver: roles of different cell populations. Int Sch Res Not 2012;2012:979480. 
  25. Valdes-Dapena MA. An atlas of foetal and neonatal histology. Philadelphia, PA: J.P. Lippincott Co; 1957. p. 75-87. 
  26. Abbaszadeh H, Ghorbani F, Derakhshani M, Movassaghpour A, Yousefi M. Human umbilical cord mesenchymal stem cell-derived extracellular vesicles: a novel therapeutic paradigm. J Cell Physiol 2020;235:706-17. 
  27. Giancotti A, Monti M, Nevi L, Safarikia S, D'Ambrosio V, Brunelli R, Pajno C, Corno S, Di Donato V, Musella A, Chiappetta MF, Bosco D, Panici PB, Alvaro D, Cardinale V. Functions and the emerging role of the foetal liver into regenerative medicine. Cells 2019;8:914. 
  28. El Baz H, Demerdash Z, Kamel M, Hammam O, Abdelhady DS, Mahmoud S, Hassan S, Mahmoud F, Atta S, Riad NM, Gaafar T. Induction of hepatic regeneration in an experimental model using hepatocyte-differentiated mesenchymal stem cells. Cell Reprogram 2020;22:134-46.