Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of substitution of soybean meal with rapeseed meal and glutamine supplementation on growth performance, intestinal morphology, and intestinal mucosa barrier of Qiandongnan Xiaoxiang Chicken

  • Zhang, Bolin (Department of Biology and Agriculture, Characteristic Laboratory of Animal Resources Conservation and Utilization of Chishui River Basin, Zunyi Normal College) ;
  • Liu, Ning (Department of Biology and Agriculture, Characteristic Laboratory of Animal Resources Conservation and Utilization of Chishui River Basin, Zunyi Normal College) ;
  • Hao, Meilin (Department of Biology and Agriculture, Characteristic Laboratory of Animal Resources Conservation and Utilization of Chishui River Basin, Zunyi Normal College) ;
  • Xie, Yuxiao (Department of Biology and Agriculture, Characteristic Laboratory of Animal Resources Conservation and Utilization of Chishui River Basin, Zunyi Normal College) ;
  • Song, Peiyong (Department of Biology and Agriculture, Characteristic Laboratory of Animal Resources Conservation and Utilization of Chishui River Basin, Zunyi Normal College)
  • Received : 2021.10.13
  • Accepted : 2022.03.23
  • Published : 2022.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: The present study was to evaluate the effects of different rapeseed meal substitution (RSM) and glutamine (Gln) supplementation on growth performance, intestine morphology, and intestinal mucosa barrier of broilers. Methods: Four hundred and twenty Qiandongnan Xiaoxiang Chicken at 1 day of age with similar weight were chosen and were randomly assigned into 7 groups, consisting of 10 replicates per group and 6 broilers per replicate. Three groups were provided with diets separately containing 0%, 10%, and 20% RSM, and the other four groups were fed with diets separately supplemented with 0.5% and 1% Gln based on the inclusion of 10% and 20% RSM. At 21 and 42 days of age, 10 broilers per group were chosen to collect plasma and intestinal samples for further analysis. Results: The results showed that 10% RSM decreased average daily feed intake (ADFI) and average daily weight gain (ADG) of broilers at 21 days of age (p<0.05). Furthermore, both ADFI and ADG of broilers at 21 and 42 days of age were decreased by 20% RSM, while feed conversion ratio (FCR) was increased (p<0.05). Besides, 10% RSM resulted in lower intestinal villus height and the ratio of villus height to crypt depth, deeper crypt depth (p<0.05), combined with the lower mRNA expressions of occludin, claudin-1, and zonula occludens-1 (ZO-1) in broilers at 21 days of age (p<0.05). Similar results were also observed in broilers at 21 and 42 days of age fed with 20% RSM. However, 1% Gln improved the growth performance of broilers fed with 10% and 20% RSM (p<0.05), ameliorated intestine morphology and elevated mRNA expressions of occludin, claudin-1 and ZO-1 (p<0.05). Conclusion: In conclusion, the increasing inclusion of RSM resulted in more serious effects on broilers, however, 1.0% Gln could reverse the negative effects induced by the inclusion of RSM.

Keywords

Acknowledgement

The authors are grateful to Rongjiang Shannong Development Co., Ltd for proving Qiandongnan Xiaoxiang chicken. The study was supported by the Basic Project of Guizhou Provincial Natural Science Foundation (Qian Kehe [2017]1205), Zunyi 15851 Talent Project (2050020213#), Zunyi City School Joint Fund (Zunshi Kehe [2018]No.08), the Rural Industrial Revolution Project of Guizhou Province (Zunshi he Rural Industry 201905), Zunshi Chi Shuihe Fund (Zunshi CSHKJ [2019]-01).

References

  1. Tuunainen P, Koivunen E, Valaja J, Valkonen E, Hiidenhovi J, Tupasela T. Effects of dietary rapeseed meal and peas on the performance and meat quality of broilers. Agric Food Sci 2016;25:22-33. https://doi.org/10.23986/afsci.52299
  2. Rutkowski A, Kaczmarek SA, Hejdysz M, Nowaczewski S, Jamroz D. Concentrates made from legume seeds (lupinus angustifolius, lupinus luteus and pisum sativum) and rapeseed meal as protein sources in laying hen diets. Ann Anim Sci 2015;15:129-42. https://doi.org/10.2478/aoas-2014-0061
  3. Zhu YW, Yang WC, Liu W, et al. Effects of dietary rapeseed meal inclusion levels on growth performance, organ weight, and serum biochemical parameters in Cherry Valley ducks. Poult Sci 2019;98:6888-96. https://doi.org/10.3382/ps/pez419
  4. Mejicanos G, Sanjayan N, Kim IH, Nyachoti CM. Recent advances in canola meal utilization in swine nutrition. J Anim Sci Technol 2016;58:7. https://doi.org/10.1186/s40781-016-0085-5
  5. Moraes PO, Novelini L, Krabbe EL, et al. Growth performance, morphometric analysis of the intestinal mucosa and thyroid of broiler fed canola meal. Arq Bras Med Vet Zootec 2018;70:187-94. https://doi.org/10.1590/1678-4162-9080
  6. Rabie MH, Hayam MA, Abo E-M, El-Gogary MR, Marwa SA. Nutritional and physiological effects of different levels of canola meal in broiler chick diets. Asian J Anim Vet Adv 2015;10:161-72. https://doi.org/10.3923/ajava.2015.161.172
  7. McNeill L, Bernard K, MacLeod MG. Food intake, growth rate, food conversion and food choice in broilers fed on diets high in rapeseed meal and pea meal, with observations on sensory evaluation of the resulting poultry meat. Br Poult Sci 2004;45:519-23. https://doi.org/10.1080/00071660412331286235
  8. Tripathi MK, Mishra AS. Glucosinolates in animal nutrition: a review. Anim Feed Sci Technol 2017;132:1-27. https://doi.org/10.1016/j.anifeedsci.2006.03.003
  9. Gopinger E, Xavier EG, Elias MC, et al. The effect of different dietary levels of canola meal on growth performance, nutrient digestibility, and gut morphology of broiler chickens. Poult Sci 2014;93:1130-6. https://doi.org/10.3382/ps.2013-03426
  10. Hu Y, Wang Y, Li A, et al. Effects of fermented rapeseed meal on antioxidant functions, serum biochemical parameters and intestinal morphology in broilers. Food Agric Immunol 2016;27:182-93. https://doi.org/10.1080/09540105.2015.1079592
  11. Gao T, Zhao M, Zhang L, et al. In ovo feeding of L-arginine regulates intestinal barrier functions of posthatch broilers by activating the mTOR signaling pathway. J Sci Food Agric 2018;98:1416-25. https://doi.org/10.1002/jsfa.8609
  12. Uni Z, Tako E, Gal-Garber O, Sklan D. Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo. Poult Sci 2003;82:1747-54. https://doi.org/10.1093/ps/82.11.1747
  13. Wang B, Wu G, Zhou Z, et al. Glutamine and intestinal barrier function. Amino Acids 2015;47:2143-54. https://doi.org/10.1007/s00726-014-1773-4
  14. Kim MH, Kim H. The roles of glutamine in the intestine and its implication in intestinal diseases. Int J Mol Sci 2017;18: 1051. https://doi.org/10.3390/ijms18051051
  15. Bartell SM, Batal AB. The Effect of supplemental glutamine on growth performance, development of the gastrointestinal tract, and humoral immune response of broilers. Poult Sci 2007;86:1940-7. https://doi.org/10.1093/ps/86.9.1940
  16. Wu Q, Liu N, Wu X, Wang G, Lin L. Glutamine alleviates heat stress-induced impairment of intestinal morphology, intestinal inflammatory response, and barrier integrity in broilers. Poult Sci 2018;97:2675-83. https://doi.org/10.3382/ps/pey123
  17. Xing S, Zhang B, Lin M, et al. Effects of alanyl-glutamine supplementation on the small intestinal mucosa barrier in weaned piglets. Asian-Australas J Anim Sci 2017;30:236-45. https://doi.org/10.5713/ajas.16.0077
  18. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  19. Woyengo TA, Kiarie E, Nyachoti CM. Growth performance, organ weights, and blood parameters of broilers fed diets containing expeller-extracted canola meal. Poult Sci 2011; 90:2520-27. https://doi.org/10.3382/ps.2011-01436
  20. Zhang B, Liu N, Yang Q, et al. Effects of 20% rapeseed meal and glutamine supplementation on intestine indices and immune function of Qiandongnan Xiaoxiang chicken. Jiangsu J Agric Sci 2020;36:1255-64 (in Chinese). https://doi.org/10.3969/j.issn.1000-4440.2020.05.024
  21. Sina P, Parviz F, Negin D. Growth performance, organ weights and, blood parameters of broilers fed diets containing graded levels of dietary canola meal and supplemental copper. J Poult Sci 2013;50:354-63. https://doi.org/10.2141/jpsa.0130006
  22. Min YN, Hancock A, Yan F, et al. Use of combinations of canola meal and distillers dried grains with solubles in broiler starter diets. J Appl Poult Res 2009;18:725-33. https://doi.org/10.3382/japr.2009-00050
  23. Dai SF, Wang LK, Wen AY, Wang LX, Jin GM. Dietary glutamine supplementation improves growth performance, meat quality and colour stability of broilers under heat stress. Br Poult Sci 2009;50:333-40. https://doi.org/10.1080/00071660902806947
  24. Xue G, Barekatain R, Wu S, Choct M, Swick RA. Dietary L-glutamine supplementation improves growth performance, gut morphology, and serum biochemical indices of broiler chickens during necrotic enteritis challenge. Poult Sci 2018;97: 1334-41. https://doi.org/10.3382/ps/pex444
  25. Zhang BL, Yang Q, Liu N, et al. Effects of dietary L-glutamine supplementation on plasma biochemical parameters, immune performance, intestinal inflammatory factors expressions and mucosal immune of broilers challenged by lipopolysaccharide. Chinese J Anim Nutr 2020;32:2611-23. https://doi.org/10.3969/j.issn.1006-267x.2020.06.020
  26. Chiang G, Lu WQ, Piao XS, Hu JK, Gong LM, Thacker PA. Effects of feeding solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. Asian-Australas J Anim Sci 2010;23:263-71. https://doi.org/10.5713/ajas.2010.90145
  27. Viveros A, Chamorro S, Pizarro M, Arija I, Centeno C, Brenes A. Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult Sci 2011;90:566-78. https://doi.org/10.3382/ps.2010-00889
  28. Gilani S, Howarth GS, Kitessa SM, Forder REA, Tran CD, Hughes RJ. New biomarkers for intestinal permeability induced by lipopolysaccharide in chickens. Anim Prod Sci 2016;56:1984-97. https://doi.org/10.1071/AN15725
  29. Turner JR, Buschmann MM, Romero-Calvo I, Sailer A, Shen L. The role of molecular remodeling in differential regulation of tight junction permeability. Semi Cell Dev Biol 2014;36:204-12. https://doi.org/10.1016/j.semcdb.2014.09.022
  30. Han X, Fink MP, Delude RL. Proinflammatory cytokines cause NO*-dependent and -independent changes in expression and localization of tight junction proteins in intestinal epithelial cells. Shock 2003;19:229-37. https://doi.org/10.1097/00024382-200303000-00006
  31. Turner JR. Intestinal mucosal barrier function in health and disease. Nat Rev Immunol 2009;9:799-809. https://doi.org/10.1038/nri2653
  32. Achamrah N, Dechelotte P, Coeffier M. Glutamine and the regulation of intestinal permeability: from bench to bedside. Curr Opin Clin Nutr Metab Care 2017;20:86-91. https://doi.org/10.1097/MCO.0000000000000339
  33. Souba WW, Klimberg VS, Plumley DA, et al. The role of glutamine in maintaining a healthy gut and supporting the metabolic response to injury and infection. J Surg Res 1990; 48:383-91. https://doi.org/10.1016/0022-4804(90)90080-l
  34. Wang H, Zhang C, Wu G, et al. Glutamine enhances tight junction protein expression and modulates corticotropinreleasing factor signaling in the jejunum of weanling piglets. J Nutr 2015;145:25-31. https://doi.org/10.3945/jn.114.202515
  35. Beutheu S, Ghouzali I, Galas L, Dechelotte P, Coeffier M. Glutamine and arginine improve permeability and tight junction protein expression in methotrexate-treated Caco-2 cells. Clin Nutr (Edinburgh, Scotland) 2013;32:863-9. https://doi.org/10.1016/j.clnu.2013.01.014
  36. DeMarco VG, Li N, Thomas J, West CM, Neu J. Glutamine and barrier function in cultured Caco-2 epithelial cell monolayers. J Nutr 2003;133:2176-9. https://doi.org/10. 1093/jn/133.7.2176 https://doi.org/10.1093/jn/133.7.2176