Journal of Interdisciplinary Genomics

Interdisciplinary Society of Genetic & Genomic Medicine (유전의학융합회)
권호 표지
DOI QR코드

DOI QR Code

Microarray Analysis of Extracranial Arteriovenous Malformation Endothelial Cells

  • Lee, Joon Seok (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Oh, Eun Jung (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Kim, Hyun Mi (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University) ;
  • Kwak, Suin (BK21 FOUR KNU Convergence Educational Program of Biomedical Science for Creative Future Talents, Department of Biomedical Science, School of Medicine, Kyungpook National University) ;
  • Lee, Seok-Jong (Department of Dermatology, School of Medicine, Kyungpook National University) ;
  • Lee, Jongmin (Department of Radiology, School of Medicine, Kyungpook National University) ;
  • Huh, Seung (Department of Surgery, School of Medicine, Kyungpook National University) ;
  • Kim, Ji Yoon (Department of Pediatrics, School of Medicine, Kyungpook National University) ;
  • Chung, Ho Yun (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University)
  • Received : 2022.10.01
  • Accepted : 2022.10.13
  • Published : 2022.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Arteriovenous malformations (AVMs) are rare diseases comprising abnormally dilated arteries and veins with an absence of a capillary network. Since these diseases are intractable after diagnosis, various treatment strategies have been examined, with continuous efforts to identify target genes. Here, we report relevant new target genes selected via gene microarray. Methods: Endothelial cells were isolated from samples collected from three patients with AVM and three healthy individuals, followed by microarray analysis. Additionally, quantitative PCR was performed to select genes highly relevant to AVM. Results: In the vascular endothelial cells derived from the tissues of patients with AVM, the expression of ANGPT1, ANGPT2, DLL4, IL6, NRG1, TGFBR1, and VEGFA was typically higher compared to those derived from normal tissues. Conclusion: Seven candidate genes were selected to analyze the pathophysiological mechanism of AVM. These results may aid in future directions of diagnosis and treatment.

Keywords

Acknowledgement

This research was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2020R1A2C2009496).

References

  1. Holzman LB, Marks RM, Dixit VM. A novel immediate early response gene of endothelium is induced by cytokines and encodes a secreted protein. Mol Cell Biol 1990;10:5830-38.
  2. Pandey A, Shao H, Marks RM, Polverini PJ, Dixit VM. Roles of B61, the ligand for the Eck receptor tyrosine kinase, in YNF-α-induced angiogenesis. Science 1995;268:567-69 https://doi.org/10.1126/science.7536959
  3. Takahashi H, Ikeda T. Molecular cloning and expression of rat and mouse B61 gene: implications on organogenesis. Oncogene 1995;11:879-83.
  4. Challa VR, Moody DM, Brown WR. Vascular malformations of the central nervous system. J Neuropath Exp Neurol 1995;54:609-21. https://doi.org/10.1097/00005072-199509000-00001
  5. Mandybur TI, Nazek M. Cerebral arteriovenous malformations. A detailed morphological and immunohistochemical study using actin. Arch Pathol Lab Med 1990;114:970-73.
  6. The arteriovenous malformation study group. Arteriovenous malformations of the brain in adults. New Eng J Med 1999;340:1812-18. https://doi.org/10.1056/NEJM199906103402307
  7. Goumans MJ, Zwijsen A, Ten Dijke P, Bailly S. Bone morphogenetic proteins in vascular homeostasis and disease. Cold Spring Harb Perspect Biol 2018;10:a031989. https://doi.org/10.1101/cshperspect.a031989
  8. Noshiro S, Mikami T, Kataoka-Sasaki Y, Sasaki M, Hashi K, Ohtaki S, et al. Biological relevance of tissue factor and IL-6 in arteriovenous malformations. Neurosurg Rev 2017;40:359-67. https://doi.org/10.1007/s10143-016-0780-1
  9. Gui C, Zeng ZY, Chen Q, Luo YW, Li L, Chen LL. Neuregulin-1 promotes myocardial angiogenesis in the rat model of diabetic cardiomyopathy. Cell Physiol Biochem 2018;46:2325-34. https://doi.org/10.1159/000489622
  10. Ryu JY, Park TH, Lee JS, Oh EJ, Kim HM, Lee SJ, et al. Wall shear stress on vascular smooth muscle cells exerts angiogenic effects on extracranial arteriovenous malformations. Arch Plast Surg 2022;49:115-20. https://doi.org/10.5999/aps.2021.00626
  11. Kim DG, Cho HG, Ryu JY, Lee JS, Lee SJ, Lee JM, et al. Clinical assessment and management of auricular arteriovenous malformation: retrospective study. Arch Craniofac Surg 2021;22:141-47. https://doi.org/10.7181/acfs.2021.00192
  12. Kim JB, Lee JW, Choi KY, Yang JD, Cho BC, Lee SJ, et al. Clinical characteristics of Arteriovenous malformations of the head and neck. Dermatol Surg 2017;43:526-33. https://doi.org/10.1097/DSS.0000000000000993
  13. Ryu JY, Kim YH, Lee JS, Lee JW, Oh EJ, Kim HM, et al. Oscillatory shear stress promotes angiogenic effects in arteriovenous malformations endothelial cells. Mol Med 2021;27:31.
  14. Lee JS, Cho HG, Ryu JY, Oh EJ, Kim HM, Kwak S, et al. Hypoxia Promotes Angiogenic Effect in Extracranial Arteriovenous Malformation Endothelial Cells. Int J Mol Sci 2022;23:9109. https://doi.org/10.3390/ijms23169109