Journal of Web Engineering

River Publishers (River Publishers)
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Chemical, Pharmacological and Computerized Molecular Analysis of Stem's Extracts of Bauhinia scandens L. Provide Insights into the Management of Diarrheal and Microbial Infections

  • Md Minarul Islam (Department of Biomedical Sciences and Institute for Medical Science, Jeonbuk National University Medical School) ;
  • Rashedul Alam (Department of Medical Science, Jeonbuk National University Medical School) ;
  • Hea-Jong Chung (Gwanju Center, Korea Basic Science Institute) ;
  • Nazim Uddin Emon (Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong) ;
  • Mohammad Fazlul Kabir (Department of Biology, Georgia State University) ;
  • Sajib Rudra (Department of Botany, Faculty of Biological Sciences, University of Chittagong) ;
  • Safaet Alam (Department of Pharmacy, State University of Bangladesh) ;
  • Ahsan Ullah (Department of Medical Science, Jeonbuk National University Medical School) ;
  • Seong-Tshool Hong (Department of Biomedical Sciences and Institute for Medical Science, Jeonbuk National University Medical School) ;
  • Mohammed Aktar Sayeed (Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong)
  • Received : 2021.12.06
  • Accepted : 2022.01.04
  • Published : 20220000
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Abstract

Bauhinia scandens L. (Family: Fabaceae) is commonly used to treat cholera, diarrhea, asthma, and diabetes disorder in integrative medicine. This study aimed to screen the presence of phytochemicals (preliminary and UPLC-QTOF-M.S. analysis) and to examine the pharmacological activities of Bauhinia scandens L. stems (MEBS) stem extracts. Besides, in silico study was also implemented to elucidate the binding affinity and drug capability of the selected phytochemicals. In vivo anti diarrheal activity was investigated in mice models. In vitro, antibacterial and antifungal properties of MEBS against several pathogenic strains were evaluated using the disc diffusion method. In addition, in silico study has been employed using Discovery studio 2020, UCFS Chimera, PyRx autodock vina, and online tools. In the anti-diarrheal investigation, MEBS showed a significant dose-dependent inhibition rate in all three methods. The antibacterial and antifungal screening showed a remarkable zone of inhibition, of the diameter 14-26 mm and 12-28 mm, by MEBS. The present study revealed that MEBS has remarkable anti-diarrheal potential and is highly effective in wide-spectrum bacterial and fungal strains. Moreover, the in silico study validated the results of biological screenings. To conclude, MEBS is presumed to be a good source in treating diarrhea, bacterial and fungal infections.

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Acknowledgement

The authors want to thank the Department of Pharmacy, International Islamic University Chittagong, for providing lab facilities, and Bokhtear Uddin (Professor, Department of Botany, University of Chittagong) for identifying the plant material. Authors are also grateful to the Fatema Akter for her heartfelt cooperation and support.