Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지
DOI QR코드

DOI QR Code

Protective Role of Corticosterone against Hydrogen Peroxide-Induced Neuronal Cell Death in SH-SY5Y Cells

  • Lee, Chan (Department of Pharmacology, School of Medicine, Keimyung University) ;
  • Jang, Jung-Hee (Department of Pharmacology, School of Medicine, Keimyung University) ;
  • Park, Gyu Hwan (College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University)
  • Received : 2022.09.29
  • Accepted : 2022.10.06
  • Published : 2022.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

Stress breaks body balance, which can cause diverse physiological disorders and worsen preexisting diseases. However, recent studies have reported that controllable stress and overcoming from stress reinforce resilience to resist against more intense stress afterwards. In this study, we investigated the protective effect of corticosterone (CORT), a representative stress hormone against hydrogen peroxide (H2O2)-induced neuronal cell death and its underlying molecular mechanism in SH-SY5Y cells, a human neuroblastoma cell line. The decreased cell viability by H2O2 was effectively restored by the pretreatment with low concentration of CORT (0.03 μM for 72 h) in the cells. H2O2-increased expression of apoptotic markers such as PUMA and Bim was decreased by CORT pretreatment. Furthermore, pretreatment of CORT attenuated H2O2-mediated oxidative damages by upregulation of antioxidant enzymes via activation of nuclear factor erythroid 2-related factor 2 (Nrf2). These findings suggest that low concentration of CORT with eustressed condition enhances intracellular self-defense against H2O2-mediated oxidative cell death, suggesting a role of low concentration of CORT as one of key molecules for resilience and neuronal cell survival.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korean Government (NRF-2019R1F1A1063005; NRF-2022R1A2C1012031).

References

  1. Aalling, N., Hageman, I., Miskowiak, K., Orlowski, D., Wegener, G. and Wortwein, G. (2018) Erythropoietin prevents the effect of chronic restraint stress on the number of hippocampal CA3c dendritic terminals-relation to expression of genes involved in synaptic plasticity, angiogenesis, inflammation, and oxidative stress in male rats. J. Neurosci. Res. 96, 103-116. https://doi.org/10.1002/jnr.24107
  2. Chen, Z. and Zhong, C. (2014) Oxidative stress in Alzheimer's disease. Neurosci. Bull. 30, 271-281. https://doi.org/10.1007/s12264-013-1423-y
  3. Faraji, J., Lehmann, H., Metz, G. A. and Sutherland, R. J. (2009) Stress and corticosterone enhance cognitive recovery from hippocampal stroke in rats. Neurosci. Lett. 462, 248-252. https://doi.org/10.1016/j.neulet.2009.06.096
  4. Gao, H., Zheng, W., Li, C. and Xu, H. (2021) Isoform-specific effects of apolipoprotein E on hydrogen peroxide-induced apoptosis in human induced pluripotent stem cell (iPSC)-derived cortical neurons. Int. J. Mol. Sci. 22, 11582. https://doi.org/10.3390/ijms222111582
  5. He, W. B., Zhao, M., Machida, T. and Chen, N. H. (2009) Effect of corticosterone on developing hippocampus: short-term and long-term outcomes. Hippocampus 19, 338-349. https://doi.org/10.1002/hipo.20523
  6. Herman, J. P., Mcklveen, J. M., Ghosal, S., Kopp, B., Wulsin, A., Makinson, R., Scheimann, J. and Myers, B. (2016) Regulation of the hypothalamic-pituitary-adrenocortical stress response. Compr. Physiol. 6, 603-621.
  7. Jiang, Y., Botchway, B. O. A., Hu, Z. and Fang, M. (2019) Overexpression of SIRT1 inhibits corticosterone-induced autophagy. Neuroscience 411, 11-22. https://doi.org/10.1016/j.neuroscience.2019.05.035
  8. Li, X. H., Liu, N. B., Zhang, M. H., Zhou, Y. L., Liao, J. W., Liu, X. Q. and Chen, H. W. (2007) Effects of chronic multiple stress on learning and memory and the expression of Fyn, BDNF, TrkB in the hippocampus of rats. Chin. Med. J. (Engl.) 120, 669-674.
  9. Liao, R., Zhang, J., Zhao, H., Zhang, Z. and Yang, K. (2020) Protective effect of low-dose hydrocortisone on myocardium in early septic shock. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 32, 210-214.
  10. Lu, S., Wei, F. and Li, G. (2021) The evolution of the concept of stress and the framework of the stress system. Cell Stress 5, 76-85. https://doi.org/10.15698/cst2021.06.250
  11. Madrigal, J. L., Garcia-Bueno, B., Caso, J. R., Perez-Nievas, B. G. and Leza, J. C. (2006) Stress-induced oxidative changes in brain. CNS Neurol. Disord. Drug Targets 5, 561-568. https://doi.org/10.2174/187152706778559327
  12. Mcneill-Blue, C., Wetmore, B. A., Sanchez, J. F., Freed, W. J. and Merrick, B. A. (2006) Apoptosis mediated by p53 in rat neural AF5 cells following treatment with hydrogen peroxide and staurosporine. Brain Res. 1112, 1-15. https://doi.org/10.1016/j.brainres.2006.07.024
  13. Sahin, E. and Gumuslu, S. (2007) Immobilization stress in rat tissues: alterations in protein oxidation, lipid peroxidation and antioxidant defense system. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 144, 342-347. https://doi.org/10.1016/j.cbpc.2006.10.009
  14. Samarghandian, S., Azimi-Nezhad, M., Borji, A., Samini, M. and Farkhondeh, T. (2017) Protective effects of carnosol against oxidative stress induced brain damage by chronic stress in rats. BMC Complement. Altern. Med. 17, 249. https://doi.org/10.1186/s12906-017-1753-9
  15. Tahera, Y., Meltser, I., Johansson, P., Hansson, A. C. and Canlon, B. (2006) Glucocorticoid receptor and nuclear factor-kappa B interactions in restraint stress-mediated protection against acoustic trauma. Endocrinology 147, 4430-4437. https://doi.org/10.1210/en.2006-0260
  16. Ye, J., Han, Y., Chen, X., Xie, J., Liu, X., Qiao, S. and Wang, C. (2014) L-carnitine attenuates H2O2-induced neuron apoptosis via inhibition of endoplasmic reticulum stress. Neurochem. Int. 78, 86-95. https://doi.org/10.1016/j.neuint.2014.08.009
  17. Zhang, H., Davies, K. J. A. and Forman, H. J. (2015) Oxidative stress response and Nrf2 signaling in aging. Free Radic. Biol. Med. 88, 314-336. https://doi.org/10.1016/j.freeradbiomed.2015.05.036