한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제32권4호
  • /
  • Pages.1-8
  • /
  • 2022
  • /
  • 1229-1854(pISSN)
  • /
  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
권호 표지
DOI QR코드

DOI QR Code

고지방식이 유도 비만 마우스에서 천마 추출물의 항비만 효과

Anti-Obesity Effects of Gastrodia elata Extracts on High Fat Diet-Induced Obese Mice

  • 김예슬 (원광대학교 한의과대학 생리학교실) ;
  • 김하림 (원광대학교 한의과대학 생리학교실) ;
  • 박은희 (원광대학교 한의과대학 생리학교실) ;
  • 송영은 (전라북도 농업기술원) ;
  • 김창수 (전라북도 농업기술원) ;
  • 하원배 (원광대학교 한의과대학 한방재활의학교실) ;
  • 우현준 (원광대학교 한의과대학 한방재활의학교실) ;
  • 한윤희 (원광대학교 한의과대학 한방재활의학교실) ;
  • 이정한 (원광대학교 한의과대학 한방재활의학교실)
  • Kim, Ye-Seul (Department of Physiology, School of Korean Medicine, Wonkwang University) ;
  • Kim, Ha-Rim (Department of Physiology, School of Korean Medicine, Wonkwang University) ;
  • Park, Eun-Hee (Department of Physiology, School of Korean Medicine, Wonkwang University) ;
  • Song, Young-Eun (Jeollabuk-Do Agricultural Research & Extension Services) ;
  • Kim, Chang-Su (Jeollabuk-Do Agricultural Research & Extension Services) ;
  • Ha, Won-Bae (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • Woo, Hyeon-Jun (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • Han, Yun-Hee (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University) ;
  • Lee, Jung-Han (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University)
  • 투고 : 2022.10.06
  • 심사 : 2022.10.07
  • 발행 : 2022.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives This study is to investigate the effects and mechanisms of Gastrodia elata extract (GEE) on the high-fat diet-induced obesity model. Methods C57BL/6 mice were randomly assigned into 5 groups (n=10). Control group was fed normal diet (ND). Obesity group was fed 60% high fat diet (HFD). The other three groups were fed HFD with 100, 200, 500 mg/kg GEE. After five weeks, body weight, liver and epididymal fat weight, triglyceride concentration in liver and serum, sterol regulatory element-binding protein-1 (SREBP-1), acetyl-CoA carboxylase (ACC), fatty acid synthase, peroxisome proliferator-activated receptor 𝛾 (PPAR-𝛾), CCAAT/enhancer binding protein 𝛼 (C/EBP-𝛼) expression level, insulin concentration in serum were measured. Results The GEE (100, 200, and 500 mg/kg)-treated animals exhibited substantial decreases in body mass, liver weight and epididymal white adipose tissue collate to the HFD-fed group. GEE treatment also reduced hepatic and serum triglyceride level. Furthermore, GEE treatment significantly inhibited adipogenesis in the GEE group by reducing the protein expression of SREBP-1, ACC and the messenger RNA expression of PPAR𝛾, C/EBP-𝛼, which are adipocyte differentiation-related genes. Conclusions These research outcomes recommend that GEE is possibly valuable for the prevention of HFD-induced obesity via modification of various pathways related with adipogenesis and adipocyte differentiation.

키워드

과제정보

본 연구는 2021년도 지역특화기술개발연구사업 지원에 의한 연구임(Project No. PJ016156).

참고문헌

  1. The Society of Korean Medicine Rehabilitation. Korean Rehabilitation Medicine. 5th ed. Paju:Globooks. 2020:322-39.
  2. Ministry of Health and Welfare, Korea Disease Control and Prevention Agency. Korea health statistics [Internet] 2019 [updated 2020 Dec 31; cited 2021 Nov 30]. Available from: URL: http://www.index.go.kr/potal/main/EachDtlPageDetail.do?idx_cd=2705.
  3. Spiegelman BM, Flier JS. Adipogenesis and obesity: rounding out the big picture. Cell. 1996;87:377-89. https://doi.org/10.1016/S0092-8674(00)81359-8
  4. Moseti D, Regassa A, Kim WK. Molecular regulation of adipogenesis and potential anti-adipogenic bioactive molecules. Int J Mol Sci. 2016;17(1):124. https://doi.org/10.3390/ijms17010124
  5. Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Gonzalez FJ, Spiegelman BM. C/EBPα induces adipogenesis through PPARγ: a unified pathway. Genes Dev. 2002;16(1):22-6. https://doi.org/10.1101/gad.948702
  6. Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol. 2006;7(12):885-96. https://doi.org/10.1038/nrm2066
  7. Czech MP, Tencerova M, Pedersen DJ, Aouadi M. Insulin signalling mechanisms for triacylglycerol storage. Diabetologia. 2013;56(5):949-64. https://doi.org/10.1007/s00125-013-2869-1
  8. Kim HC. Herbal medicine pharmacology. Seoul: Jipmoondang. 2001:391-3.
  9. Herbology Editorial Committee of Korean Medicine Schools. Boncho-hak [Herbology]. Seoul:Yeonglimsa. 1991:504-5.
  10. Huang NK, Lin YL, Cheng JJ, Lai WL. Gastrodia elata prevents rat pheochromocytoma cells from serum-deprived apoptosis: the role of the MAPK family. Life Sci. 2004;75(13):1649-57. https://doi.org/10.1016/j.lfs.2004.05.008
  11. Wu CR, Hsieh MT, Huang SC, Peng WH, Chang YS, Chen CF. Effects of Gastrodia elata and its active constituents on scopolamine-induced amnesia in rats. Planta Medica. 1996;62(4):317-21. https://doi.org/10.1055/s-2006-957892
  12. Sim KC. Effect of each Gastrodia elata Blume concentration on antidiabetic in diabetic mellitus rats. Korean J Orient Physiol Pathol. 2007;21:1477-82.
  13. Cho HH, Kwon DK, Kim JW, Song YJ. Effect of Salicornia herbacea L. supplementation on tissue triglyceride concentrations and PGC-1α & PPAR-γ expression of skeletal muscle of rats fed a high-fat diet. Journal of Life Science. 2018;28(7):857-63. https://doi.org/10.5352/JLS.2018.28.7.857
  14. Lee SJ, Kang MH, Min HS. Folic acid supplementation reduces oxidative stress and hepatic toxicity in rats treated chronically with ethanol. Nutrition Research and Practice. 2011;5(6):520-6. https://doi.org/10.4162/nrp.2011.5.6.520
  15. Korean Endocrine Society, Korean Society for the Study of Obesity. Management of obesity, 2010 recommendation. Endocrinol Metab. 2010;25(4):301-4. https://doi.org/10.3803/EnM.2010.25.4.301
  16. Kersten S. Mechanisms of nutritional and hormonal regulation of lipogenesis. EMBO Reports. 2001;2(4):282-6. https://doi.org/10.1093/embo-reports/kve071