International Journal of Stem Cells

  • 제15권4호
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  • Pages.372-383
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  • 2022
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  • 2005-3606(pISSN)
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  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
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LIPUS Promotes Endothelial Differentiation and Angiogenesis of Periodontal Ligament Stem Cells by Activating Piezo1

  • Rui Hu (Department of Preventive Dentistry, College of Stomatology, Chongqing Medical University) ;
  • Zheng-yan Yang (Department of Preventive Dentistry, College of Stomatology, Chongqing Medical University) ;
  • Yue-heng Li (Department of Preventive Dentistry, College of Stomatology, Chongqing Medical University) ;
  • Zhi Zhou (Department of Preventive Dentistry, College of Stomatology, Chongqing Medical University)
  • 투고 : 2022.02.03
  • 심사 : 2022.04.18
  • 발행 : 2022.11.30
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초록

Background and Objectives: Low-intensity pulsed ultrasound (LIPUS) promotes differentiation and regulates biological functions of various stem cells, but its effect on the endothelial differentiation of periodontal ligament stem cells (PDLSCs) is unclear. This study investigated the effect of LIPUS on endothelial differentiation and angiogenesis in PDLSCs and the role of the mechanically sensitive ion channel Piezo1 in this process. Methods and Results: PDLSCs obtained from healthy people were used for endothelial induction, and 10 ㎍/ml lipopolysaccharide (LPS) was used to simulate the inflammatory state. The induced cells were treated with LIPUS (50 mW/cm2, 1.5 MHz) to study its effect on the endothelial differentiation of PDLSCs and the tube formation of differentiated cells. PCR, flow cytometry, immunofluorescence, and Matrigel tube formation assays were used to detect the differentiation and tube formation of PDLSCs. GsMTx4 was used to inhibit the expression of Piezo1, and the role of the Piezo1 pathway in the endothelial differentiation and microvascular formation of PDLSCs after LIPUS treatment was studied. The data showed that LIPUS increased endothelial differentiation and angiogenesis in PDLSCs under inflammatory or noninflammatory conditions. The use of an inhibitor weakened the effect of LIPUS. Conclusions: This study demonstrated that LIPUS can activate the expression of Piezo1 and promote the endothelial differentiation and microvascular formation of PDLSCs.

키워드

과제정보

This study was supported by the Natural Science Foundation Project of Chongqing (CSTC2019jcyj-msxmX0191), Science Project of the Chongqing Municipal Health Commission (Grant No.2021MSXM31), and the Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016 (Grant No. CXTDG201602006).

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