International Journal of Stem Cells

Korean Society for Stem Cell Research (한국줄기세포학회)
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Effects and Mechanisms of Bone Marrow Mesenchymal Stem Cell Transplantation for Treatment of Ischemic Stroke in Hypertensive Rats

  • Yulin Liu (Department of Rehabilitation Medicine, Panyu Central Hospital) ;
  • Ying Zhao (South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital) ;
  • Yu Min (Department of Rehabilitation Medicine, Panyu Central Hospital) ;
  • Kaifeng Guo (Department of Rehabilitation Medicine, Panyu Central Hospital) ;
  • Yuling Chen (Department of Rehabilitation Medicine, Panyu Central Hospital) ;
  • Zhen Huang (Department of Rehabilitation Medicine, Panyu Central Hospital) ;
  • Cheng Long (South China Normal University-Panyu Central Hospital Joint Laboratory of Translational Medical Research, Panyu Central Hospital)
  • Received : 2021.08.02
  • Accepted : 2021.09.24
  • Published : 2022.05.30
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Abstract

Background and Objectives: Stroke is the most common cause of human death and functional disability, resulting in more than 5 million deaths worldwide each year. Bone marrow mesenchymal stem cells (BMSCs) are a kind of stem cell that are able to self-renew and differentiate into many types of tissues. Therefore, BMSCs have the potential to replace damaged neurons and promote the reconstruction of nerve conduction pathways and connective tissue. However, it remains unknown whether transplanted BMSCs promote angiogenesis or improve the tissue microenvironment directly or indirectly through paracrine interactions. This study aimed to determine the therapeutic effect of BMSCs on ischemic stroke with hypertension in a rodent model and to explore the possible mechanisms underlying any benefits. Methods and Results: Middle cerebral artery occlusion was used to establish the experimental stroke model. The area of cerebral infarction, expression of vascular endothelial growth factor (VEGF) and glial cell line-derived neurotrophic factor (GDNF), and increment of astrocyte were measured by TTC staining, western blot, real-time quantitative polymerase chain reaction (RT-qPCR) and immunocytochemistry. The results showed a smaller area of cerebral infarction and improved neurological function scores in animals treated with BMSCs compared to controls. The results of RT-qPCR and western blot assays showed higher expression of VEGF and GDNF in BMSC-treated animals compared with controls. Our study also showed that one round of BMSCs transplantation significantly promoted the proliferation of subventricular zone and cortical cells, especially astrocytes, on the ischemic side following cerebral ischemia. Conclusions: Above findings support that BMSCs have therapeutic effects for ischemic stroke complicated with hypertension, which may occur via up-regulated expression of VEGF and GDNF and reduction of neuronal apoptosis, thereby promoting the recovery of nerve function.

Keywords

Acknowledgement

This work has been financially supported by Panyu District Science and Technology Planning Project (No: 2018-Z04-07). The Experimental Center of the School of Life Sciences of South China Normal University provided the facilities for this study.

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