International Journal of Stem Cells

  • 제15권2호
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  • Pages.173-182
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  • 2022
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  • 2005-3606(pISSN)
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  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
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Single-Cell RNA Sequencing of Bone Marrow Mesenchymal Stem Cells from the Elderly People

  • Dezhou Zhu (Department of Orthopaedics, The Third Affiliated Hospital of Wenzhou Medical University) ;
  • Jie Gao (Department of Orthopaedics, The Seventh Medical Center of Chinese PLA General Hospital) ;
  • Chengxuan Tang (Department of Orthopaedics, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou) ;
  • Zheng Xu (Department of Outpatient, The First Retired Cadre Sanitarium of Beijing Garrison in Fengtai District) ;
  • Tiansheng Sun (Department of Orthopaedics, The Seventh Medical Center of Chinese PLA General Hospital)
  • 투고 : 2021.03.06
  • 심사 : 2021.08.24
  • 발행 : 2022.05.30
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초록

Background and Objectives: Bone marrow mesenchymal stem cells (BMSCs) show considerable promise in regenerative medicine. Many studies demonstrated that BMSCs cultured in vitro were highly heterogeneous and composed of diverse cell subpopulations, which may be the basis of their multiple biological characteristics. However, the exact cell subpopulations that make up BMSCs are still unknown. Methods and Results: In this study, we used single-cell RNA sequencing (scRNA-Seq) to divide 6,514 BMSCs into three clusters. The number and corresponding proportion of cells in clusters 1 to 3 were 3,766 (57.81%), 1,720 (26.40%), and 1,028 (15.78%). The gene expression profile and function of the cells in the same cluster were similar. The vast majority of cells expressed the markers defining BMSCs by flow cytometry and gene expression analysis. Each cluster had at least 20 differentially expressed genes (DEGs). We conducted Gene Ontology enrichment analysis on the top 20 DEGs of each cluster and found that the three clusters had different functions, which were related to self-renewal, multilineage differentiation and cytokine secretion, respectively. In addition, the function of the top 20 DEGs of each cluster was checked by the National Center for Biotechnology Information gene database to further verify our hypothesis. Conclusions: This study indicated that scRNA-Seq can be used to divide BMSCs into different subpopulations, demonstrating the heterogeneity of BMSCs.

키워드

과제정보

This work was supported by the special project for innovation of military medical research plan (16CXZ002) and the Beijing Nova Program (xx2018021).

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