International Journal of Stem Cells

Korean Society for Stem Cell Research (한국줄기세포학회)
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Transcriptomic Comparison Analysis between Ameloblastoma and AM-1 Cell Line

  • Shujin Li (Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry) ;
  • Dong-Joon Lee (Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry) ;
  • Hyun-Yi Kim (NGeneS Inc.) ;
  • Hidemitsu Harada (Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University) ;
  • Young-Soo Jung (Department of Oral & Maxillofacial Surgery, Yonsei University College of Dentistry) ;
  • Han-Sung Jung (Division in Anatomy and Developmental Biology, Department of Oral Biology, Taste Research Center, Oral Science Research Center, BK21 FOUR Project, Yonsei University College of Dentistry)
  • Received : 2022.07.30
  • Accepted : 2022.09.13
  • Published : 2022.11.30
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Abstract

Cancer initiation and progression are profoundly along with the crosstalk between cancer cells and the surrounding stroma. Accumulating evidence has shown that the therapy targeting the extracellular matrix (ECM) would regress tumor growth and invasion in the most common carcinomas. However, it remains largely unexplored in several rare tumors like odontogenic tumors. Ameloblastoma (AM) is the representative odontogenic epithelial tumor in the jawbone, and it usually infiltrates into adjacent bone marrow and has unlimited growth capacity and a high potential for recurrence. This study aims to investigate the role of collagen-rich ECM during the invasion of AM. Transcriptomic analysis revealed that ECM- and epithelial-to-mesenchymal transition (EMT)-related genes were up-regulated in AM compared to ameloblastoma cell line, AM-1. Tumoroid forming analysis showed that Collagen-rich ECM is indispensable for AM progression, especially for aggressive growth patterns and collective invasion.

Keywords

Acknowledgement

This study was supported by the Yonsei University College of Dentistry Fund (6-2019-0014). The skillful assistance for visualizing RNA-seq data of Junsoo Song (NGeneS Inc.) is gratefully acknowledged.

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