Genomics & Informatics

Korea Genome Organization (한국유전체학회)
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NBLAST: a graphical user interface-based two-way BLAST software with a dot plot viewer

  • Received : 2022.08.22
  • Accepted : 2022.09.05
  • Published : 2022.09.30
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Abstract

BLAST, a basic bioinformatics tool for searching local sequence similarity, has been one of the most widely used bioinformatics programs since its introduction in 1990. Users generally use the web-based NCBI-BLAST program for BLAST analysis. However, users with large sequence data are often faced with a problem of upload size limitation while using the web-based BLAST program. This proves inconvenient as scientists often want to run BLAST on their own data, such as transcriptome or whole genome sequences. To overcome this issue, we developed NBLAST, a graphical user interface-based BLAST program that employs a two-way system, allowing the use of input sequences either as "query" or "target" in the BLAST analysis. NBLAST is also equipped with a dot plot viewer, thus allowing researchers to create custom database for BLAST and run a dot plot similarity analysis within a single program. It is available to access to the NBLAST with http://nbitglobal.com/nblast.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2020R1I1A3052662). We thanks Mr. Alexander Jungik Hong (Cheongshim International Academy, Republic of Korea) that tested the operation of the NBLAST software.

References

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