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Rapid micropropagation of wild garlic (Allium victorialis var. platyphyllum) by the scooping method

  • Jeong, Mi Jin (Plant Conservation Division, Korea National Arboretum of the Korea Forest Service) ;
  • Yong, Seong Hyeon (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Kim, Do Hyeon (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Park, Kwan Been (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science)) ;
  • Kim, Hak Gon (Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute) ;
  • Choi, Pil Son (Department of Medicinal Plant Resources, Nambu University) ;
  • Choi, Myung Suk (Divisition of Environmental Forest Science, Gyeongsang National University (Institute of Agriculture of Life Science))
  • 투고 : 2022.07.24
  • 심사 : 2022.08.02
  • 발행 : 2022.09.30

초록

Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

키워드

과제정보

This research was funded by the Forestry Science and Technology Research and Development Project (Forest Convergence Specialist Training Project), Project No. (2020186A00-2022-AA02).

참고문헌

  1. Alejandro FAB (2016) Development of in vitro techniques as supportive tools for breeding and mass clonal propagation of Leucocoryne spp. (Amaryllidaceae). Thesis of Doctor, Pontificia Uni-versidad Catolica de Chile
  2. Ayabe M, Sumi S (1998) Establishment of a novel tissue culture method, stem-disc culture, and its practical application to micropropagation of garlic (Allium sativum L.). Plant Cell Rep 17:773-779 https://doi.org/10.1007/s002990050481
  3. Choi SJ, Yoon BS, Kim JC, Kang WH (2006) Plant regeneration from Allium victorialis plant parts. J Agr Sci 17:17-22
  4. De Klerk GJ (2012) Micropropagation of bulbous crops: Technology and present state. Floric Ornam Biotechnol 6:1-8
  5. Hong MP, Lee HJ, Chun YM, Hong BR (2010) Flora of Mt. Seorak, Gangwon-do. Korean J Environ Ecol 24:436-486
  6. Jeong MJ, Song HJ, Sim SJ, Doh ES, Chang JP, Yang JK, Yun CW, Lee CH, Karigar CS, Choi MS (2012) Influence of nitrogen, phosphorous, potassium and sucrose on the shoot and bulb growth of Allium victorialis var. platyphyllum. Korean J Med Crop Sci 20:101-107 https://doi.org/10.7783/KJMCS.2012.20.2.101
  7. Kamstaityte D, Stanys V (2004) Micropropagation of onion (Allium cepa L.). Acta Univ Latviensis Biol 676:173-176
  8. Kim MJ, Park YM, Park SB (2014) Wild garlic cultivation technology. National Institute of Forest Science (In Korean)
  9. Kumar S, Sharadamma KC, Radhakrishna PM (2010) Effects of a garlic active based growth promoter on growth performance and specific pathogenic intestinal microbial counts of broiler chicks. Int J Poult Sci 9:244-246 https://doi.org/10.3923/ijps.2010.244.246
  10. Kwon YH, Jeong JH, Lee JS, Jeon JO, Park YU, Min JH, Chang WB, Lee SY, Youn CK, Kim KH (2018) Multiple shoot induction and bulb mass proliferation system by in vitro immature spathe culture of elephant garlic (Allium ampeloprasum L.). Korean J Plant Resour 31:355-362
  11. Lee CH, Jeong MJ (2018) In vitro culture and shoot proliferation of wild garlic(Allium ochotense Prokh.). J Agric Life Sci 52:73-80
  12. Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol Plant 29:92-96 https://doi.org/10.1007/BF02632259
  13. Maesato K, Sharada K, Fukui H, Hara T, Sarma KS (1994) In vitro bulblet regeneration from bulb scale explants of Lilium japonicum Thunb. Effect of plant growth regulators and culture environment. J Hortic Sci 69:289-297 https://doi.org/10.1080/14620316.1994.11516457
  14. Memon N (2012) In vitro propagation of gladiolus plantlets and cormels. J Hortic Sci Ornam Plants 4:280-291
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267-275 https://doi.org/10.1007/BF02822732
  17. Nam SI, Park JH, Choi JI, Kwon KS, Uhm JS (2002) Commercial production of seed garlic by tissue culture technique. J Plant Biotechnol 29:171-177 https://doi.org/10.5010/JPB.2002.29.3.171
  18. Ohnuki-Tierney E (1981) Illness and Healing Among the Sakhalin Ainu: A Symbolic Interpretation (preview). Cambridge University Press. pp.192
  19. Park YJ, Heo BG, Jeong SY, Jeong JH, An MS (1998) Effective and economical propagation method of Lycoris squamigera native to Korea. Hortic Sci Technol 16:242-243
  20. Paulos MM, Khandagale VG, Nadkarni BH (2015) Effect of IAA and IBA on In vitro Rooting of Banana (Musa paradisiaca) Cv.Grand Naine. Int J Sci and Res 4: 959-962
  21. Quoirin M, Lepoivre P (1977) Improved media for in vitro culture of Prunus sp. Acta Hortic 78:437-442 https://doi.org/10.17660/ActaHortic.1977.78.54
  22. Saniewski M (1977) Effect of ethrel and morphactin on bulblet differentiation in scooped out hyacinth (Hyacinthus orientalis L.) bulbs. Bull Acad Pol Sci Biol 25:107-110
  23. Xu Z, Um YC, Kim CH, Lu G, Guo DP, Liu HL, Bah AA, Mao A (2008) Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation. Acta Physiol Plant 30:521-528 https://doi.org/10.1007/s11738-008-0150-x
  24. Zheng SJ, Henken B, Krens FA, Kik C (2003) The development of an efficient cultivar-independent plant regeneration system from callus derived from both apical and non-apical root segments of garlic (Allium sativum L.). In Vitro Cell Dev Biol Plant 39:288-292