Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-Graying Effect of Pueraria Lobata Root Extract on Stress-Induced Hair Graying

갈근 추출물의 스트레스성 백모 형성 억제 효과

  • Hong, Min Jung (Basic Research & Innovation Division, AMOREPACIFIC Corporation, R&I Center) ;
  • Park, Byung Cheol (Department of Dermatology, Dankook Medical College) ;
  • Hong, Yong Deog (Basic Research & Innovation Division, AMOREPACIFIC Corporation, R&I Center) ;
  • Kim, Su Na (Basic Research & Innovation Division, AMOREPACIFIC Corporation, R&I Center)
  • 홍민정 (아모레퍼시픽 기술연구원, 기반혁신연구 Division) ;
  • 박병철 (단국대학교 피부과) ;
  • 홍용덕 (아모레퍼시픽 기술연구원, 기반혁신연구 Division) ;
  • 김수나 (아모레퍼시픽 기술연구원, 기반혁신연구 Division)
  • Received : 2022.09.20
  • Accepted : 2022.09.30
  • Published : 2022.09.30
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Abstract

Gray hair is a representative sign of aging. Intrinsic aging, stress, and the external environment cause hair graying. Stress is known to be a major factor in the early onset of hair graying. We previously found that Pueraia lobata root extract (PLRE) can prevent hair graying by promoting melanin formation. However, it remains unknown whether PLRE can prevent hair graying induced by conditions of stress. In this study, we confirmed the effect of PLRE on stress-induced hair graying. A reporter cell line was newly constructed to confirm the expression of microphthalamia-associated transcription factor (MITF), the main transcription factor for melanin production. MITF expression and melanin pigmentation were reduced in human hair follicle tissue treated with the stress hormone cortisol or H2O2 to induce oxidative stress. PLRE treatment restored MITF expression and increased the amount of melanin pigment in the hair follicle. The expression of Tyrosinase related proteins-2 (TRP-2), a melanin synthesis enzyme in the hair follicle, also increased. In conclusion, PLRE can effectively prevent the inhibition of melanin synthesis by stress hormones and oxidative stress.

흰머리는 대표적인 노화의 징후이자 피부, 모발 노화의 특징이다. 내인성 노화, 스트레스, 외부 환경으로 인해 백모가 발생하는데, 조기 백모 발생에는 스트레스가 주요한 요인이 된다고 알려져 있다. 과거 우리는 갈근 추출물이 멜라닌 형성 촉진을 통해 백모를 케어를 할 수 있음을 밝힌 바 있으며, 이에 갈근 추출물이 스트레스에 의한 백모 형성 또한 예방 할 수 있을지 본 연구를 통해 확인하였다. 연구를 위해 멜라닌 생성 주요 전사인자인 MITF 발현을 확인 할 수 있는 세포주를 구축하였다. 스트레스 호르몬 cortisol과 산화 스트레스 유발 인자 H2O2를 처리할 경우 MITF 발현이 감소하고, 인체 모낭 조직에서 멜라닌 색소가 감소함을 확인하였다. 여기에 갈근 추출물을 함께 처리할 경우 MITF 발현과 모낭 내 멜라닌 색소 량이 다시 증가하였다. 뿐만 아니라 모낭 내 멜라닌 합성에 관여하는 효소인 TRP-2의 발현도 증가함을 확인할 수 있었다. 이러한 결과를 통해 갈근 추출물은 스트레스 호르몬과 산화 스트레스 자극에 의한 멜라닌 합성 저해도 효과적으로 막아줄 수 있음을 입증하였다.

Keywords

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