Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Protective Effect of Gatrodiae Rhizoma Extracts on the LPS-Induced Cognitive Impairment Model

LPS에 의해 유도된 인지기능 손상모델에 대한 천마 추출물의 방어효과

  • Kwon, Kang-Beom (Department of Physiology, College of Korean Medicine, Wonkwang University) ;
  • Kim, Ha-Rim (Department of Physiology, College of Korean Medicine, Wonkwang University) ;
  • Kim, Ye-Seul (Department of Physiology, College of Korean Medicine, Wonkwang University) ;
  • Park, Eun-Hee (Department of Physiology, College of Korean Medicine, Wonkwang University) ;
  • Kang, Hyung-Won (Department of Neuropsychiatry, College of Korean Medicine, Wonkwang University) ;
  • Ryu, Do-Gon (Department of Physiology, College of Korean Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김하림 (원광대학교 한의과대학 생리학교실) ;
  • 김예슬 (원광대학교 한의과대학 생리학교실) ;
  • 박은희 (원광대학교 한의과대학 생리학교실) ;
  • 강형원 (원광대학교 한의과대학 신경정신과학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Received : 2022.08.22
  • Accepted : 2022.09.22
  • Published : 2022.09.30
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Abstract

Objectives: Gastrodia elata (GE) has been used to treat cognition impairment, including Alzheimer's disease (AD) in Korea. The purpose of this study was to investigate the effects of GE water extracts (GEE) on the lipopolysaccharide (LPS)-induced AD model in mice. (Aβ). Methods: We classified six groups as follow; group 1: control (CON), group 2: LPS (0.5 mg/kg/day, four times), group 3: 4 mg/kg donepezil (DP), group 4: 100 mg/kg GEE+LPS, group 5: 200 mg/kg GEE+LPS, group 6: 500 mg/kg GEE+LPS. Results: We found that GEE has an effect that inhibits decrease of discrimination index in object recognition test, as well as spontaneous alteration in the Y-maze test by LPS. Treatment with LPS increased amlyloid-β (Aβ) concentration, and decreased brain-derived neurotrophic factor (BDNF) in cerebral cortex of mice. However, GEE significantly protected against LPS-induced Aβ and BDNF changes. Our findings also showed that the inflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β)] mRNA and protein were up-regulated by the LPS injection. But GEE significantly suppressed LPS-induced inflammatory cytokines increase in a dose-dependent manner. Conclusions: This study suggests that the GEE may be an effective AD therapeutic agent, in treating neurodegenerative diseases including AD.

Keywords

Acknowledgement

This study was supported by grant of the Wonkwang University in 2020.

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