International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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A preliminary study of the anti-inflammatory activities of the Japanese oak silk moth, Antheraea yamamai

  • Park, Seung-Won (Department of Biomedical Science, Daegu Catholic University)
  • Received : 2022.08.26
  • Accepted : 2022.09.20
  • Published : 2022.09.30
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Abstract

The present study aimed to determine whether a hemolymph prepared from Antheraea yamamai larvae had the same biological activities using a Bombyx mori hemolymph prior to exposure to lipopolysaccharide (LPS) in order to induce an inflammatory response. The effects of the hemolymph were determined using a reverse transcription-quantitative polymerase chain reaction to assess the expression of pro-inflammatory molecules. The A. yamamai hemolymph exerted anti-inflammatory effects on LPS-activated human monocytic leukemia cells via Toll-like receptor (TLR) 4-mediated suppression, similar to the B. mori hemocyte extract. Treatment with the A. yamamai hemolymph significantly suppressed LPS-induced upregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression at all tested concentrations compared with the control, similar to the B. mori immune-challenged hemolymph. Finally, the A. yamamai hemolymph, like the B. mori immune-challenged hemolymph, suppressed all of these concentrations in a dose-independent manner. These results demonstrate that the hemolymph of A. yamamai exhibited important biologically active substances. Further in-depth functional studies are required to fully understand the mechanisms underlying the biological activities of wild-type silkworm hemolymphs.

Keywords

Acknowledgement

I would like to acknowledge and thank Dr. Seong Ryul Kim and Dr. Kee-Young Kim at the NIAS for their role in technical support and A. yamamai larvae collecting samples. I also express sincerely my former research assistants from the Department of Biotechnology, at the Daegu Catholic University and gratitude to the participants who were involved in the study. This work was supported by the National Research Foundation of Korea (NRF) grant founded by the Korea Government (MSIT) (No. 2021R1F1A1062456).

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