International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Identification of domesticated silkworm varieties using single nucleotide polymorphisms detected from mitochondrial genomes

  • Park, Jong Woo (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Jeong Sun (Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Jeong, Chan Young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kang, Sang Kuk (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Seong-Wan (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Nam-Suk (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Kee Young (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Iksoo (Department of Applied Biology, College of Agriculture & Life Sciences, Chonnam National University)
  • Received : 2022.08.22
  • Accepted : 2022.09.26
  • Published : 2022.09.30
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Abstract

Silkworms have recently attracted attention as healthy functional foods. Different varieties of silkworms have functional differences; thus, there is an emerging need for variety identification. In this study, we sequenced complete mitochondrial genomes (mitogenomes) of ten government-recommended silkworm varieties (BaekHwang, BaekOk, DaeBaek, DaeBak, DaeHwang, GoldenSilk, HanSaeng, JooHwang, KumKang, and KumOk). Comparison of these sequences allowed us to select the single nucleotide polymorphisms (SNPs) in 34 sites that are specific to six silkworm varieties: 13 in DaeBak, 8 in GoldenSilk, 9 in KumKang, 2 in BaekHwang, 1 in BaekOk, and 1 in DaeHwang. Among these each one SNP per variety was amplified by preparing variety-specific primers and then using tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR). As a result, it was possible to identify these six varieties among the ten silkworm varieties, evidencing that SNPs developed from mitogenomes are useful marker for the discrimination of genetically closer silkworm varieties.

Keywords

Acknowledgement

This study was supported by the 2022 RDA fellowship program of National Institute of Agricultural Science and was supported by a grant (No. PJ01510703) from the Rural Development Administration, Republic of Korea.

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