Nutrition Research and Practice

  • 제16권5호
  • /
  • Pages.589-603
  • /
  • 2022
  • /
  • 1976-1457(pISSN)
  • /
  • 2005-6168(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지
DOI QR코드

DOI QR Code

Effect of blended protein nutritional support on reducing burn-induced inflammation and organ injury

  • Yu, Yonghui (China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University) ;
  • Zhang, Jingjie (China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University) ;
  • Wang, Jing (China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University) ;
  • Wang, Jing (Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs) ;
  • Chai, Jiake (Burn Institute, the Fourth Medical Center of PLA General Hospital)
  • 투고 : 2021.08.25
  • 심사 : 2022.03.25
  • 발행 : 2022.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

BACKGROUND/OBJECTIVES: Previous studies have reported that protein supplementation contributes to the attenuation of inflammation. Serious trauma such as burn injury usually results in the excessive release of inflammatory factors and organs dysfunction. However, a few reports continued to focus on the function of protein ingestion in regulating burn-induced inflammation and organ dysfunction. MATERIALS/METHODS: This study established the rat model of 30% total body surface area burn injury, and evaluated the function of blended protein (mixture of whey and soybean proteins). Blood routine examination, inflammatory factors, blood biochemistry, and immunohistochemical assays were employed to analyze the samples from different treatment groups. RESULTS: Our results indicated a decrease in the numbers of white blood cells, monocytes, and neutrophils in the burn injury group administered with the blended protein nutritional support (Burn+BP), as compared to the burn injury group administered normal saline supplementation (Burn+S). Expressions of the pro-inflammatory factors (tumor necrosis factor-α and interleukin-6 [IL-6]) and chemokines (macrophage chemoattractant protein-1, regulated upon activation normal T cell expressed and secreted factor, and C-C motif chemokine 11) were dramatically decreased, whereas anti-inflammatory factors (IL-4, IL-10, and IL-13) were significantly increased in the Burn+BP group. Kidney function related markers blood urea nitrogen and serum creatinine, and the liver function related markers alanine transaminase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase were remarkably reduced, whereas albumin levels were elevated in the Burn+BP group as compared to levels obtained in the Burn+S group. Furthermore, inflammatory cells infiltration of the kidney and liver was also attenuated after burn injury administered with blended protein supplementation. CONCLUSIONS: In summary, nutritional support with blended proteins dramatically attenuates the burn-induced inflammatory reaction and protects organ functions. We believe this is a new insight into a potential therapeutic strategy for nutritional support of burn patients.

키워드

과제정보

This work was supported by the Beijing Nova Program (Z181100006218043), the Beijing Technology and Innovation Plan (Z191100009318015), the Innovation Engineering Project of the Chinese Academy of Agricultural Sciences, the Beijing Natural Science Foundation (7172210), and the National Natural Science Foundation of China (NSFC81471873).

참고문헌

  1. Yu Y, Gaine GK, Zhou L, Zhang J, Wang J, Sun B. The classical and potential novel healthy functions of rice bran protein and its hydrolysates. Crit Rev Food Sci Nutr. Forthcoming 2021.
  2. Liberman K, Njemini R, Luiking Y, Forti LN, Verlaan S, Bauer JM, Memelink R, Brandt K, Donini LM, Maggio M, et al. Thirteen weeks of supplementation of vitamin D and leucine-enriched whey protein nutritional supplement attenuates chronic low-grade inflammation in sarcopenic older adults: the PROVIDE study. Aging Clin Exp Res 2019;31:845-54. https://doi.org/10.1007/s40520-019-01208-4
  3. Shin JE, Park SJ, Ahn SI, Choung SY. Soluble whey protein hydrolysate ameliorates muscle atrophy induced by immobilization via regulating the PI3K/Akt pathway in C57BL/6 mice. Nutrients 2020;12:3362.
  4. Zhang J, Yu Y, Wang J. Protein nutritional support: the classical and potential new mechanisms in the prevention and therapy of sarcopenia. J Agric Food Chem 2020;68:4098-108. https://doi.org/10.1021/acs.jafc.0c00688
  5. Yu Y, Zhang J, Wang J, Sun B. MicroRNAs: the novel mediators for nutrient-modulating biological functions. Trends Food Sci Technol 2021;114:167-75. https://doi.org/10.1016/j.tifs.2021.05.028
  6. Yu Y, Zhou L, Li X, Liu J, Li H, Gong L, Zhang J, Wang J, Sun B. The progress of nomenclature, structure, metabolism, and bioactivities of oat novel phytochemical: avenanthramides. J Agric Food Chem 2022;70:446-57. https://doi.org/10.1021/acs.jafc.1c05704
  7. Bitzer ZT, Wopperer AL, Chrisfield BJ, Tao L, Cooper TK, Vanamala J, Elias RJ, Hayes JE, Lambert JD. Soy protein concentrate mitigates markers of colonic inflammation and loss of gut barrier function in vitro and in vivo. J Nutr Biochem 2017;40:201-8. https://doi.org/10.1016/j.jnutbio.2016.11.012
  8. Da Silva MS, Bigo C, Barbier O, Rudkowska I. Whey protein hydrolysate and branched-chain amino acids downregulate inflammation-related genes in vascular endothelial cells. Nutr Res 2017;38:43-51. https://doi.org/10.1016/j.nutres.2017.01.005
  9. Reidy PT, Walker DK, Dickinson JM, Gundermann DM, Drummond MJ, Timmerman KL, Cope MB, Mukherjea R, Jennings K, Volpi E, et al. Soy-dairy protein blend and whey protein ingestion after resistance exercise increases amino acid transport and transporter expression in human skeletal muscle. J Appl Physiol (1985) 2014;116:1353-64. https://doi.org/10.1152/japplphysiol.01093.2013
  10. Ren G, Yi S, Zhang H, Wang J. Ingestion of soy-whey blended protein augments sports performance and ameliorates exercise-induced fatigue in a rat exercise model. Food Funct 2017;8:670-9. https://doi.org/10.1039/C6FO01692H
  11. Liu J, Klebach M, Visser M, Hofman Z. Amino acid availability of a dairy and vegetable protein blend compared to single casein, whey, soy, and pea proteins: a double-blind, cross-over trial. Nutrients 2019;11:2613.
  12. Butteiger DN, Cope M, Liu P, Mukherjea R, Volpi E, Rasmussen BB, Krul ES. A soy, whey and caseinate blend extends postprandial skeletal muscle protein synthesis in rats. Clin Nutr 2013;32:585-91. https://doi.org/10.1016/j.clnu.2012.10.001
  13. Ren G, Zhang J, Li M, Tang Z, Yang Z, Cheng G, Wang J. Gut microbiota composition influences outcomes of skeletal muscle nutritional intervention via blended protein supplementation in posttransplant patients with hematological malignancies. Clin Nutr 2021;40:94-102. https://doi.org/10.1016/j.clnu.2020.04.030
  14. Yu Y, Chai J, Zhang H, Chu W, Liu L, Ma L, Duan H, Li B, Li D. miR-194 Promotes burn-induced hyperglycemia via attenuating IGF-IR expression. Shock 2014;42:578-84. https://doi.org/10.1097/SHK.0000000000000258
  15. Li X, Liu L, Yang J, Yu Y, Chai J, Wang L, Ma L, Yin H. Exosome derived from human umbilical cord mesenchymal stem cell mediates MiR-181c attenuating burn-induced excessive inflammation. EBioMedicine 2016;8:72-82. https://doi.org/10.1016/j.ebiom.2016.04.030
  16. Liu L, Yu Y, Hou Y, Chai J, Duan H, Chu W, Zhang H, Hu Q, Du J. Human umbilical cord mesenchymal stem cells transplantation promotes cutaneous wound healing of severe burned rats. PLoS One 2014;9:e88348.
  17. Zhao F, Liu W, Yu YH, Liu XQ, Yin HN, Liu LY, Yi GF. Effect of small molecular weight soybean proteinderived peptide supplementation on attenuating burn injury-induced inflammation and accelerating wound healing in a rat model. RSC Advances 2019;9:1247-59. https://doi.org/10.1039/C8RA09036J
  18. Yu Y, Yang L, Han S, Wu Y, Liu L, Chang Y, Wang X, Chai J. MIR-190B alleviates cell autophagy and burn-induced skeletal muscle wasting via modulating PHLPP1/Akt/FoxO3A signaling pathway. Shock 2019;52:513-21. https://doi.org/10.1097/SHK.0000000000001284
  19. Zhao F, Yu Y, Liu W, Zhang J, Liu X, Liu L, Yin H. Small Molecular weight soybean protein-derived peptides nutriment attenuates rat burn injury-induced muscle atrophy by modulation of ubiquitinproteasome system and autophagy signaling pathway. J Agric Food Chem 2018;66:2724-34. https://doi.org/10.1021/acs.jafc.7b05387
  20. Pecht T, Gutman-Tirosh A, Bashan N, Rudich A. Peripheral blood leucocyte subclasses as potential biomarkers of adipose tissue inflammation and obesity subphenotypes in humans. Obes Rev 2014;15:322-37. https://doi.org/10.1111/obr.12133
  21. Rondanelli M, Klersy C, Terracol G, Talluri J, Maugeri R, Guido D, Faliva MA, Solerte BS, Fioravanti M, Lukaski H, et al. Whey protein, amino acids, and vitamin D supplementation with physical activity increases fat-free mass and strength, functionality, and quality of life and decreases inflammation in sarcopenic elderly. Am J Clin Nutr 2016;103:830-40. https://doi.org/10.3945/ajcn.115.113357
  22. Sakuma M, Khan MA, Yasuhara S, Martyn JA, Palaniyar N. Mechanism of pulmonary immunosuppression: extrapulmonary burn injury suppresses bacterial endotoxin-induced pulmonary neutrophil recruitment and neutrophil extracellular trap (NET) formation. FASEB J 2019;33:13602-16. https://doi.org/10.1096/fj.201901098R
  23. Chen Z, Bozec A, Ramming A, Schett G. Anti-inflammatory and immune-regulatory cytokines in rheumatoid arthritis. Nat Rev Rheumatol 2019;15:9-17. https://doi.org/10.1038/s41584-018-0109-2
  24. Haller H, Bertram A, Nadrowitz F, Menne J. Monocyte chemoattractant protein-1 and the kidney. Curr Opin Nephrol Hypertens 2016;25:42-9. https://doi.org/10.1097/MNH.0000000000000186
  25. Fichna M, Zurawek M, Budny B, Komarowska H, Niechcial E, Fichna P, Ruchala M. Elevated serum RANTES chemokine levels in autoimmune Addison disease. Pol Arch Intern Med 2018;128:216-21.
  26. Rankin SM, Conroy DM, Williams TJ. Eotaxin and eosinophil recruitment: implications for human disease. Mol Med Today 2000;6:20-7. https://doi.org/10.1016/S1357-4310(99)01635-4
  27. Yagi H, Soto-Gutierrez A, Kitagawa Y, Tilles AW, Tompkins RG, Yarmush ML. Bone marrow mesenchymal stromal cells attenuate organ injury induced by LPS and burn. Cell Transplant 2010;19:823-30. https://doi.org/10.3727/096368910X508942
  28. Inoue Y, Yu YM, Kurihara T, Vasilyev A, Ibrahim A, Oklu R, Zhao G, Nair AV, Brown D, Fischman AJ, et al. Kidney and liver injuries after major burns in rats are prevented by resolvin D2. Crit Care Med 2016;44:e241-52. https://doi.org/10.1097/CCM.0000000000001397
  29. Kaneko Y, Cho T, Sato Y, Goto K, Yamamoto S, Goto S, Madaio MP, Narita I. Attenuated macrophage infiltration in glomeruli of aged mice resulting in ameliorated kidney injury in nephrotoxic serum nephritis. J Gerontol A Biol Sci Med Sci 2018;73:1178-86. https://doi.org/10.1093/gerona/gly019
  30. Lamas-Paz A, Hao F, Nelson LJ, Vazquez MT, Canals S, Gomez Del Moral M, Martinez-Naves E, Nevzorova YA, Cubero FJ. Alcoholic liver disease: utility of animal models. World J Gastroenterol 2018;24:5063-75. https://doi.org/10.3748/wjg.v24.i45.5063
  31. Kolodziej U, Maciejczyk M, Niklinska W, Waszkiel D, Zendzian-Piotrowska M, Zukowski P, Zalewska A. Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats. Arch Oral Biol 2017;84:6-12. https://doi.org/10.1016/j.archoralbio.2017.09.006
  32. Machin M, Simoyi MF, Blemings KP, Klandorf H. Increased dietary protein elevates plasma uric acid and is associated with decreased oxidative stress in rapidly-growing broilers. Comp Biochem Physiol B Biochem Mol Biol 2004;137:383-90. https://doi.org/10.1016/j.cbpc.2004.01.002
  33. Pan J, Shi M, Ma L, Fu P. Mechanistic insights of soluble uric acid-related kidney disease. Curr Med Chem 2020;27:5056-66. https://doi.org/10.2174/0929867326666181211094421
  34. Ng JW, Cairns SA, O'Boyle CP. Management of the lower gastrointestinal system in burn: a comprehensive review. Burns 2016;42:728-37. https://doi.org/10.1016/j.burns.2015.08.007
  35. Babajafari S, Hojhabrimanesh A, Sohrabi Z, Ayaz M, Noorafshan A, Akrami A. Comparing isolated soy protein with flaxseed oil vs isolated soy protein with corn oil and wheat flour with corn oil consumption on muscle catabolism, liver function, blood lipid, and sugar in burn patients: a randomized clinical trial. Trials 2018;19:308.
  36. Nelms CL. Optimizing enteral nutrition for growth in pediatric chronic kidney disease (CKD). Front Pediatr 2018;6:214.
  37. McGraw NJ, Krul ES, Grunz-Borgmann E, Parrish AR. Soy-based renoprotection. World J Nephrol 2016;5:233-57. https://doi.org/10.5527/wjn.v5.i3.233
  38. Li M, Zhao H, Wu J, Wang L, Wang J, Lv K, Liu S, Wang M, Guan W, Liu J, et al. Nobiletin protects against acute liver injury via targeting c-Jun N-terminal kinase (JNK)-induced apoptosis of hepatocytes. J Agric Food Chem 2020;68:7112-20. https://doi.org/10.1021/acs.jafc.0c01722
  39. Qiao M, Yang J, Zhu Y, Zhao Y, Hu J. Transcriptomics and proteomics analysis of system-level mechanisms in the liver of apigenin-treated fibrotic rats. Life Sci 2020;248:117475.
  40. Epting CL, McBride ME, Wald EL, Costello JM. Pathophysiology of post-operative low cardiac output syndrome. Curr Vasc Pharmacol 2016;14:14-23.
  41. Clark A, Imran J, Madni T, Wolf SE. Nutrition and metabolism in burn patients. Burns Trauma 2017;5:11.
  42. Klein GL. The role of the musculoskeletal system in post-burn hypermetabolism. Metabolism 2019;97:81-6. https://doi.org/10.1016/j.metabol.2019.06.001
  43. Rousseau AF, Losser MR, Ichai C, Berger MM. ESPEN endorsed recommendations: nutritional therapy in major burns. Clin Nutr 2013;32:497-502. https://doi.org/10.1016/j.clnu.2013.02.012
  44. Moore FA, Phillips SM, McClain CJ, Patel JJ, Martindale RG. Nutrition support for persistent inflammation, immunosuppression, and catabolism syndrome. Nutr Clin Pract 2017;32:121S-127S. https://doi.org/10.1177/0884533616687502
  45. Lami FH, Al Naser RK. Epidemiological characteristics of burn injuries in Iraq: a burn hospital-based study. Burns 2019;45:479-83. https://doi.org/10.1016/j.burns.2018.03.005
  46. Korkmaz HI, Ulrich MM, van Wieringen WN, Vlig M, Emmens RW, Meyer KW, Sinnige P, Krijnen PA, van Zuijlen PP, Niessen HW. The local and systemic inflammatory response in a pig burn wound model with a pivotal role for complement. J Burn Care Res 2017;38:e796-806. https://doi.org/10.1097/BCR.0000000000000486
  47. Stoppe C, Averdunk L, Goetzenich A, Soppert J, Marlier A, Kraemer S, Vieten J, Coburn M, Kowark A, Kim BS, et al. The protective role of macrophage migration inhibitory factor in acute kidney injury after cardiac surgery. Sci Transl Med 2018;10:eaan4886.