Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Pharmacological potential of Cordyceps militaris with enhanced Cordycepin production for anti-inflammatory and tumor cell anti-proliferative applications

  • Ha, Si-Young (Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jung, Ji-Young (Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Yang, Jae-Kyung (Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University)
  • Received : 2022.09.07
  • Accepted : 2022.09.20
  • Published : 2022.09.30
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Abstract

Cordyceps militaris mycelium extracts containing high amounts of cordycepin were evaluated in vitro for their anti-inflammatory and tumor cell growth-inhibitory activities. All extracts dose dependently inhibited the increased production of inflammatory mediators including reactive oxygen species (ROS), nitric oxide (NO), and 𝛽-hexosaminidase in lipopolysaccharide (LPS)-stimulated inflammatory cells. All extracts were evaluated for anti-proliferative activity against normal RBL-2H3 cells and diverse types of cancer cell lines, including HCT, MC5-7, U-87MG, AGS, and A549 cells. The extract showed the strongest growth inhibition (IC50 = 28.13 ㎍/mL) relative to vehicle-treated control cells against fibrosarcoma (MC5-7). We have demonstrated anti-inflammatory activity of C. militaris via inhibition of NO, ROS production, and 𝛽-hexosaminidase release in activated cells. C. militaris mycelium extract was also evaluated mechanistically and found to exert six types of anti-cancer activity, confirming its pharmacological potential. Our study suggests C. militaris use as a potential source of anti-inflammatory and anti-cancer agents. C. militaris may also be considered a functional food.

Keywords

Acknowledgement

This study was carried out with the support of 'R&D Program for Forest Science Technology (Project No. 2020186D10-2222-AA0261382116530003)' provided by Korea Forest Service (Korea Forestry Promotion Institute).

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