Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Dexamethasone enhances glucose uptake by SGLT1 and GLUT1 and boosts ATP generation through the PPP-TCA cycle in bovine neutrophils

  • Wang, Xinbo (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Tang, Mingyu (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Zhang, Yuming (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Li, Yansong (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Mao, Jingdong (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Deng, Qinghua (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Li, Shusen (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Jia, Zhenwei (College of Animal Science and Technology, Inner Mongolia Minzu University) ;
  • Du, Liyin (College of Animal Science and Technology, Inner Mongolia Minzu University)
  • Received : 2022.04.23
  • Accepted : 2022.07.26
  • Published : 2022.09.30
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Abstract

Background: Clinical dexamethasone (DEX) treatment or stress in bovines results in extensive physiological changes with prominent hyperglycemia and neutrophils dysfunction. Objectives: To elucidate the effects of DEX treatment in vivo on cellular energy status and the underlying mechanism in circulating neutrophils. Methods: We selected eight-month-old male bovines and injected DEX for 3 consecutive days (1 time/d). The levels of glucose, total protein (TP), total cholesterol (TC), and the proinflammatory cytokines interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α in blood were examined, and we then detected glycogen and adenosine triphosphate (ATP) content, phosphofructosekinase-1 (PFK1) and glucose-6-phosphate dehydrogenase (G6PDH) activity, glucose transporter (GLUT)1, GLUT4, sodium/glucose cotransporter (SGLT)1 and citrate synthase (CS) protein expression and autophagy levels in circulating neutrophils. Results: DEX injection markedly increased blood glucose, TP and TC levels, the Ca2+/P5+ ratio and the neutrophil/lymphocyte ratio and significantly decreased blood IL-1β, IL-6 and TNF-α levels. Particularly in neutrophils, DEX injection inhibited p65-NFκB activation and elevated glycogen and ATP contents and SGLT1, GLUT1 and GR expression while inhibiting PFK1 activity, enhancing G6PDH activity and CS expression and lowering cell autophagy levels. Conclusions: DEX induced neutrophils glucose uptake by enhancing SGLT1 and GLUT1 expression and the transformation of energy metabolism from glycolysis to pentose phosphate pathway (PPP)-tricarboxylic acid (TCA) cycle. This finding gives us a new perspective on deeper understanding of clinical anti-inflammatory effects of DEX on bovine.

Keywords

Acknowledgement

We thank the students of College of College of Animal Science and Technology of Inner Mongolia Minzu University who helped in this research and the support by the Natural Science Foundation of China, Natural Science Foundation of Inner Mongolia Autonomous Region and Young Scientific and Technological Talents in Inner Mongolia.

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