Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
권호 표지
DOI QR코드

DOI QR Code

Investigation of Antioxidant, Hypoglycemic and Anti-Obesity Effects of Euphorbia Resinifera L.

  • Benrahou, Kaoutar (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University) ;
  • El Guourrami, Otman (Laboratory of Analytical Chemistry and Bromatology, Faculty of Medicine and Pharmacy, Mohammed V University) ;
  • Mrabti, Hanae Naceiri (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University) ;
  • Cherrah, Yahia (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University) ;
  • My El Abbes, Faouzi (Laboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analyzes Research Team, Faculty of Medicine and Pharmacy, Mohammed V University)
  • Received : 2022.02.25
  • Accepted : 2022.06.16
  • Published : 2022.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The aim of this work is to evaluate the in vitro antioxidant, hypoglycemic, and antiobesity effects of Euphorbia resinifera extracts and investigate the phenolic constituents and the toxicity of these extracts. Methods: Phytochemical screening was performed to detect polyphenols and flavonoids. Antioxidant activity was evaluated by four methods (DPPH, ABTS, H2O2, and xanthine oxidase inhibition). The hypoglycemic effect was determined by the inhibition of α-amylase and α-glucosidase enzymes in vitro and via a starch tolerance study in normal rats. The antiobesity effect was estimated by in vitro inhibition of lipase. Results: Phytochemical screening revealed that the ethanolic extract was rich in polyphenols (99 ± 0.56 mg GEA/g extract) and tannins (55.22 ± 0.17 mg RE/g extract). Moreover, this extract showed higher antioxidant activity in different tests: the DPPH assay (IC50 = 53.81 ± 1.83 ㎍/mL), ABTS assay (111.4 ± 2.64 mg TE/g extract), H2O2 (IC50 = 98.15 ± 0.68 ㎍/mL), and xanthine oxidase (IC50 = 10.26 ± 0.6 ㎍/mL). With respect to hypoglycemic effect, the aqueous and ethanolic extracts showed IC50 values of 119.7 ± 2.15 ㎍/mL and 102 ± 3.63 ㎍/mL for α-amylase and 121.4 ± 1.88 and 56.6 ± 1.12 ㎍/mL for α-glucosidase, respectively, and the extracts lowered blood glucose levels in normal starch-loaded rats. Additionally, lipase inhibition was observed with aqueous (IC50 = 25.3 ± 1.53 ㎍/mL) and ethanolic (IC50 = 13.7 ± 3.03 ㎍/mL) extracts. Conclusion: These findings show the antioxidant, hypoglycemic, and hyperlipidemic effects of E. resinifera extracts, which should be investigated further to validate their medicinal uses and their pharmaceutical applications.

Keywords

References

  1. Noor ZI, Ahmed D, Rehman HM, Qamar MT, Froeyen M, Ahmad S, et al. In vitro antidiabetic, anti-obesity and antioxidant analysis of Ocimum basilicum aerial biomass and in silico molecular docking simulations with alpha-amylase and lipase enzymes. Biology (Basel). 2019;8(4):92.
  2. Ajiboye BO, Ojo OA, Okesola MA, Akinyemi AJ, Talabi JY, Idowu OT, et al. In vitro antioxidant activities and inhibitory effects of phenolic extract of Senecio biafrae (Oliv and Hiern) against key enzymes linked with type II diabetes mellitus and Alzheimer's disease. Food Sci Nutr. 2018;6(7):1803-10. https://doi.org/10.1002/fsn3.749
  3. Balasubramaniam V, Mustar S, Mustafa Khalid N, Abd Rashed A, Mohd Noh MF, Wilcox MD, et al. Inhibitory activities of three Malaysian edible seaweeds on lipase and α-amylase. J Appl Phycol. 2013;25(5):1405-12. https://doi.org/10.1007/s10811-012-9964-4
  4. Worsztynowicz P, Napierala M, Bialas W, Grajek W, Olkowicz M. Pancreatic α-amylase and lipase inhibitory activity of polyphenolic compounds present in the extract of black chokeberry (Aronia melanocarpa L.). Process Biochem. 2014;49(9):1457-63. https://doi.org/10.1016/j.procbio.2014.06.002
  5. Oboh G, Akinyemi AJ, Ademiluyi AO, Adefegha SA. Inhibitory effects of aqueous extracts of two varieties of ginger on some key enzymes linked to type-2 diabetes in vitro. J Food Nutr Res. 2010;49(1):14-20.
  6. Poongothai K, Ponmurugan P, Ahmed KS, Kumar BS, Sheriff SA. Antihyperglycemic and antioxidant effects of Solanum xanthocarpum leaves (field grown & in vitro raised) extracts on alloxan induced diabetic rats. Asian Pac J Trop Med. 2011;4(10):778-85. https://doi.org/10.1016/S1995-7645(11)60193-4
  7. Nair SS, Kavrekar V, Mishra A. In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. Eur J Exp Biol. 2013;3(1):128-32.
  8. Wang S, Liang H, Zhao Y, Wang G, Yao H, Kasimu R, et al. New triterpenoids from the latex of Euphorbia resinifera Berg. Fitoterapia. 2016;108:33-40. https://doi.org/10.1016/j.fitote.2015.11.009
  9. Boutoub O, El-Guendouz S, Estevinho LM, Paula VB, Aazza S, El Ghadraoui L, et al. Antioxidant activity and enzyme inhibitory potential of Euphorbia resinifera and E. officinarum honeys from Morocco and plant aqueous extracts. Environ Sci Pollut Res Int. 2021;28(1):503-17. https://doi.org/10.1007/s11356-020-10489-6
  10. Wang SY, Li GY, Zhang K, Wang HY, Liang HG, Huang C, et al. New ingol-type diterpenes from the latex of Euphorbia resinifera. J Asian Nat Prod Res. 2019;21(11):1075-82. https://doi.org/10.1080/10286020.2018.1498084
  11. Spanos GA, Wrolstad RE. Influence of processing and storage on the phenolic composition of Thompson Seedless grape juice. J Agric Food Chem. 1990;38(7):1565-71. https://doi.org/10.1021/jf00097a030
  12. Dewanto V, Wu X, Liu RH. Processed sweet corn has higher antioxidant activity. J Agric Food Chem. 2002;50(17):4959-64. https://doi.org/10.1021/jf0255937
  13. Huang B, Ke H, He J, Ban X, Zeng H, Wang Y. Extracts of Halenia elliptica exhibit antioxidant properties in vitro and in vivo. Food Chem Toxicol. 2011;49(1):185-90. https://doi.org/10.1016/j.fct.2010.10.015
  14. Tuberoso CI, Boban M, Bifulco E, Budimir D, Pirisi FM. Antioxidant capacity and vasodilatory properties of Mediterranean food: the case of Cannonau wine, myrtle berries liqueur and strawberry-tree honey. Food Chem. 2013;140(4):686-91. https://doi.org/10.1016/j.foodchem.2012.09.071
  15. Muruhan S, Selvaraj S, Viswanathan PK. In vitro antioxidant activities of Solanum surattense leaf extract. Asian Pac J Trop Biomed. 2013;3(1):28-34. https://doi.org/10.1016/S2221-1691(13)60019-2
  16. Umamaheswari M, AsokKumar K, Somasundaram A, Sivashanmugam T, Subhadradevi V, Ravi TK. Xanthine oxidase inhibitory activity of some Indian medical plants. J Ethnopharmacol. 2007;109(3):547-51. https://doi.org/10.1016/j.jep.2006.08.020
  17. Chakrabarti R, Singh B, Prakrith VN, Vanchhawng L, Thirumurugan K. Screening of nine herbal plants for in vitro α-amylase inhibition. Asian J Pharm Clin Res. 2014;7(4):84-9.
  18. Kee KT, Koh M, Oong LX, Ng K. Screening culinary herbs for antioxidant and α-glucosidase inhibitory activities. Int J Food Sci Technol. 2013;48(9):1884-91. https://doi.org/10.1111/ijfs.12166
  19. Hu B, Cui F, Yin F, Zeng X, Sun Y, Li Y. Caffeoylquinic acids competitively inhibit pancreatic lipase through binding to the catalytic triad. Int J Biol Macromol. 2015;80:529-35. https://doi.org/10.1016/j.ijbiomac.2015.07.031
  20. Beejmohun V, Peytavy-Izard M, Mignon C, Muscente-Paque D, Deplanque X, Ripoll C, et al. Acute effect of Ceylon cinnamon extract on postprandial glycemia: alpha-amylase inhibition, starch tolerance test in rats, and randomized crossover clinical trial in healthy volunteers. BMC Complement Altern Med. 2014;14:351. https://doi.org/10.1186/1472-6882-14-351
  21. Botham PA. Acute systemic toxicity--prospects for tiered testing strategies. Toxicol In Vitro. 2004;18(2):227-30. https://doi.org/10.1016/S0887-2333(03)00143-7
  22. Farah H, Ech-chahad A, Lamiri A. Antioxidant, antimicrobial and phytochemical investigations of polar extracts of Euphorbia resinifera Beg., roots, stems and flowers. Am J Adv Drug Deliv. 2014;2(6):776-85.
  23. Peter EL, Nagendrappa PB, Ajayi CO, Sesaazi CD. Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: modeling and optimization of extraction conditions. PLoS One. 2021;16(4):e0250405. https://doi.org/10.1371/journal.pone.0250405
  24. Dudonne S, Vitrac X, Coutiere P, Woillez M, Merillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem. 2009;57(5):1768-74. https://doi.org/10.1021/jf803011r
  25. Boulfia M, Lamchouri F, Senhaji S, Lachkar N, Bouabid K, Toufik H. Mineral content, chemical analysis, In Vitro antidiabetic and antioxidant activities, and antibacterial power of aqueous and organic extracts of Moroccan Leopoldia comosa (L.) Parl. Bulbs. Evid Based Complement Alternat Med. 2021;2021:9932291.
  26. Hanane F, Abdellah EC, Abdeslam L. In vitro antioxidant and antibacterial activity of the root extract of Euphorbia resinifera. J Pharmacogn Phytochem. 2014;2(5):161-3.
  27. Sayah K, Marmouzi I, Naceiri Mrabti H, Cherrah Y, Faouzi ME. Antioxidant activity and inhibitory potential of Cistus salviifolius (L.) and Cistus monspeliensis (L.) aerial parts extracts against key enzymes linked to hyperglycemia. Biomed Res Int. 2017;2017:2789482.
  28. Ameer K, Shahbaz HM, Kwon JH. Green extraction methods for polyphenols from plant matrices and their byproducts: a review. Compr Rev Food Sci Food Saf. 2017;16(2):295-315. https://doi.org/10.1111/1541-4337.12253
  29. Birari RB, Bhutani KK. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today. 2007;12(19-20):879-89. https://doi.org/10.1016/j.drudis.2007.07.024
  30. Singh U, Singh S, Kochhar A. Therapeutic potential of antidiabetic nutraceuticals. Phytopharmacology. 2012;2(1):144-69.
  31. Chater PI, Wilcox M, Cherry P, Herford A, Mustar S, Wheater H, et al. Inhibitory activity of extracts of Hebridean brown seaweeds on lipase activity. J Appl Phycol. 2016;28:1303-13. https://doi.org/10.1007/s10811-015-0619-0
  32. Wang SY, Huang C, Sun RK, Lu LN, Liang HG, Gao L, et al. New tirucallane triterpenoids from the dried latex of Euphorbia resinifera. Phytochem Lett. 2019;29:220-4. https://doi.org/10.1016/j.phytol.2018.12.012
  33. Gutierrez-Del-Rio I, Lopez-Ibanez S, Magadan-Corpas P, Fernandez-Calleja L, Perez-Valero A, Tunon-Granda M, et al. Terpenoids and polyphenols as natural antioxidant agents in food preservation. Antioxidants (Basel). 2021;10(8):1264. https://doi.org/10.3390/antiox10081264
  34. Chen YZ, Zhang BW, Yang J, Zou CS, Li T, Zhang GC, et al. Detoxification, antioxidant, and digestive enzyme activities and gene expression analysis of Lymantria dispar larvae under carvacrol. J Asia Pac Entomol. 2021;24(1):208-16. https://doi.org/10.1016/j.aspen.2020.12.014
  35. Yang W, Chen X, Li Y, Guo S, Wang Z, Yu X. Advances in pharmacological activities of terpenoids. Nat Prod Commun. 2020. doi: 10.1177/1934578X20903555. [Epub ahead of print]
  36. Cavar Zeljkovic S, Siskova J, Komzakova K, De Diego N, Kaffkova K, Tarkowski P. Phenolic compounds and biological activity of selected Mentha species. Plants (Basel). 2021;10(3):550.
  37. Fettach S, Mrabti HN, Sayah K, Bouyahya A, Salhi N, Cherrah Y, et al. Phenolic content, acute toxicity of Ajuga iva extracts and assessment of their antioxidant and carbohydrate digestive enzyme inhibitory effects. South Afr J Bot. 2019;125:381-5. https://doi.org/10.1016/j.sajb.2019.08.010
  38. Naceiri Mrabti H, Marmouzi I, Sayah K, Chemlal L, El Ouadi Y, Elmsellem H, et al. Arbutus unedo L aqueous extract is associated with in vitro and in vivo antioxidant activity. J Mater Environ Sci. 2017;8(1):217-24.
  39. Simeonova R, Vitcheva V, Krasteva I, Zdraveva P, Konstantinov S, Ionkova I. Antidiabetic and antioxidant effects of saponarin from Gypsophila trichotoma on streptozotocin-induced diabetic normotensive and hypertensive rats. Phytomedicine. 2016; 23(5):483-90. https://doi.org/10.1016/j.phymed.2016.02.024
  40. Sengupta S, Mukherjee A, Goswami R, Basu S. Hypoglycemic activity of the antioxidant saponarin, characterized as alphaglucosidase inhibitor present in Tinospora cordifolia. J Enzyme Inhib Med Chem. 2009;24(3):684-90. https://doi.org/10.1080/14756360802333075