ALGAE

  • 제37권3호
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  • Pages.185-204
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  • 2022
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  • 1226-2617(pISSN)
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  • 2093-0860(eISSN)
한국조류학회(藻類) (The Korean Society of Phycology)
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Morphological and molecular characterization of the genus Coolia (Dinophyceae) from Bahía de La Paz, southwest Gulf of California

  • Morquecho, Lourdes (Programa de Planeacion Ambiental y Conservacion, Centro de Investigaciones Biologicas del Noroeste (CIBNOR)) ;
  • Garate-Lizarraga, Ismael (Instituto Politecnico Nacional, Centro Interdisciplinario de Ciencias Marinas (CICIMAR-IPN)) ;
  • Gu, Haifeng (Department of Marine Biodiversity, Third Institute of Oceanography, Ministry of Natural Resources)
  • 투고 : 2022.01.24
  • 심사 : 2022.09.02
  • 발행 : 2022.09.15
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초록

The genus Coolia A. Meunier 1919 has a global distribution and is a common member of epiphytic dinoflagellate assemblages in neritic ecosystems. Coolia monotis is the type species of the genus and was the only known species for 76 years. Over the past few decades, molecular characterization has unveiled two species complexes that group morphologically very similar species, so their limits are often unclear. To provide new knowledge on the biogeography and species composition of the genus Coolia, 16 strains were isolated from Bahía de La Paz, Gulf of California. The species were identified by applying morphological and molecular approaches. The morphometric characteristics of all isolated Coolia species were consistent with the original taxa descriptions. Phylogenetic analyses (large subunit [LSU] rDNA D1 / D2 and internal transcribed spacer [ITS] 1 / 5.8S / ITS2) revealed a species assemblage comprising Coolia malayensis, C. palmyrensis, C. tropicalis, and the C. cf. canariensis lineage. This is the first report of Coolia palmyrensis and C. cf. canariensis in Mexico and C. tropicalis in the Gulf of California. Our results strengthen the biogeographical understanding of these potentially harmful epiphytic dinoflagellate species.

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과제정보

Jorge Angulo-Calvillo and Enrique Calvillo-Espinoza provided technical assistance in the field as divers and boat drivers. Amada Reyes-Salinas, Alejandra Mazariegos-Villarreal and Ariel Cruz-Villacorta provided technical assistance. Thelma Castellanos, Angel Carrillo and Goretty Caamal provided technical advice and infrastructure of the Molecular Microbial Ecology Laboratory. All are at CIBNOR. Yuri Okolodkov (Universidad Veracruzana) assisted during the 2016 macroalgae sampling. Laura E. Gomez-Lizarraga (SAMEB, ICMyL-UNAM) also provided technical assistance. This study was supported by the CIBNOR project 20014, and the CONACYT A1-S-37026 grant. IGL was supported by SIP-20190272 and COFAA. Taylor Morey provided English revision. Maria Elena Sanchez-Salazar edited the English manuscript.

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