Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Genotoxicity Evaluation Using Reversion Mutation Test of SU-Eohyeol Pharmacopuncture

SU어혈약침의 복귀돌연변이시험을 이용한 유전독성평가

  • Ku, Jaseung (Bogwang Korean Medical Clinic) ;
  • Hwang, Ji Hye (Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University)
  • 구자승 (보광한의원) ;
  • 황지혜 (가천대학교 한의과대학 침구의학과)
  • Received : 2022.05.04
  • Accepted : 2022.07.20
  • Published : 2022.08.25
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Abstract

SU-Eohyeol pharmacopuncture(SUEP) was developed by adding Cervi Pantotrichum Cornu to Jungsongouhyul pharmacopuncture. This genotoxicity evaluation was performed to evaluate the mutagenic potential of the test substance SUEP agent using histidine, which requires strains of Salmonella typhimurium (TA98, TA100, TA1535, TA1537), and tryptophan, which requires Escherichia coli (WP2uvrA) strain in the presence and absence of metabolic activation. According to the results of the dose range finding study conducted prior to the main study, the dose levels of the test substance in the main study were determined as 100, 50, 25, 12.5, 6.25%, and positive and negative controls were established. As a result of the main study, the mean number of revertant colonies compared to negative controls was less than 2-fold at all dose levels of SUEP in all strains with and without metabolic activation. In the positive control group, the mean number of revertant colonies for each strain was markedly increased by more than two times compared to the negative control group. Based on the result of this study, the test substance, SUEP did not show any indication of mutagenic potential under the conditions of this study.

Keywords

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