Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effect of KW21 and water-extracted horseradish leaf combination on Nannochloropsis sp. density in laboratory scale

  • Received : 2021.11.27
  • Accepted : 2022.06.21
  • Published : 2022.08.31
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Abstract

This study aims to know the effect of water-extracted horseradish Moringa oleifera leaf and KW21 fertilizer combination application on the density of Nannochloropsis sp. It was conducted in the Natural Food Laboratory of State Polytechnique, Tual. The experiment used complete randomized design with 5 treatments and 3 replications: A (25% horseradish leaf extract + 75% KW21), B (50% horseradish leaf extract + 50% KW21), C (75% horseradish leaf extract + 25% KW21), D (positive control of 100% KW21), and E (negative control of 100% horseradish leaf extract). Results showed that Treatment C yielded the best result, both the highest density of Nannochloropsis sp. and suitable harvest time.

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References

  1. Adam F, Abert-Vian M, Peltier G, Chemat F. "Solvent-free" ultrasound-assisted extraction of lipids from fresh microalgae cells: a green, clean and scalable process. Bioresour Technol. 2012;114:457-65. https://doi.org/10.1016/j.biortech.2012.02.096
  2. Ali M, Watson IA. Microwave treatment of wet algal paste for enhanced solvent extraction of lipids for biodiesel production. Renew Energy. 2015;76:470-7. https://doi.org/10.1016/j.renene.2014.11.024
  3. Aminah S, Ramdhan T, Yanis M. Nutrient content and functional features of horseradish plant Moringa oleifera. Buletin Pertanian Perkotaan. 2015;5:35-44.
  4. Arfah Y, Cokrowati N, Mukhlis A. The effect of urea fertilizer concentration on cell population growth of Nannochloropsis sp. J Kelaut. 2019;12:45-51.
  5. Buono S, Langellotti AL, Martello A, Rinna F, Fogliano V. Functional ingredients from microalgae. Food Funct. 2014;5:1669-85. https://doi.org/10.1039/C4FO00125G
  6. Chitranjali T, Anoop Chandran P, Muraleedhara Kurup G. Omega-3 fatty acid concentrate from Dunaliella salina possesses anti-inflammatory properties including blockade of NF-κB nuclear translocation. Immunopharmacol Immunotoxicol. 2015;37:81-9. https://doi.org/10.3109/08923973.2014.981639
  7. Cho SH, Ji SC, Hur SB, Bae J, Park IS, Song YC. Optimum temperature and salinity conditions for growth of green algae Chlorella ellipsoidea and Nannochloris oculata. Fish Sci. 2007;73:1050-6. https://doi.org/10.1111/j.1444-2906.2007.01435.x
  8. de Jesus Raposo MF, de Morais RMSC, de Morais AMMB. Health applications of bioactive compounds from marine microalgae. Life Sci. 2013;93:479-86. https://doi.org/10.1016/j.lfs.2013.08.002
  9. Enzing C, Ploeg M, Barbosa M, Sijtsma L. JRC scientific and policy reports: microalgae-based products for the food and feed sector: an outlook for Europe. Luxembourg: Publications Office of the European Union; 2014. p. 1-78.
  10. Fachrullah MR. Growth rate of biofuel-producing microalgae Chlorella sp. and Nannochloropsis sp. cultivated using waste water of the tin mining in Bangka Island [B.S. thesis]. Bogor: IPB University; 2011.
  11. Fahey JW, Fahey SD. Moringa oleifera: a review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees Life J. 2005;1:5.
  12. Gheysen L, Demets R, Devaere J, Bernaerts T, Goos P, Van Loey A, et al. Impact of microalgal species on the oxidative stability of n-3 LC-PUFA enriched tomato puree. Algal Res. 2019;40:101502.
  13. Gimpel JA, Hyun JS, Schoepp NG, Mayfield SP. Production of recombinant proteins in microalgae at pilot greenhouse scale. Biotechnol Bioeng. 2015;112:339-45. https://doi.org/10.1002/bit.25357
  14. Gu N, Lin Q, Li G, Tan Y, Huang L, Lin J. Effect of salinity on growth, biochemical composition, and lipid productivity of Nannochloropsis oculata CS 179. Eng Life Sci. 2012;12:631-7. https://doi.org/10.1002/elsc.201100204
  15. Huerlimann R, de Nys R, Heimann K. Growth, lipid content, productivity, and fatty acid composition of tropical microalgae for scale-up production. Biotechnol Bioeng. 2010;107:245-57. https://doi.org/10.1002/bit.22809
  16. Isnansetyo A, Kurniastuty. Natural phytoplankton and zooplankton feed culture techniques for hatching marine organisms. Yogyakarta: Kanisius; 1995. p. 116.
  17. Jadid R, Dewiyanti I, Nurfadillah. Addition of coconut water in growth media of Nannochloropsis sp. J Ilm Mhs Kelautan Perikanan Unsyiah. 2017;2:113-8.
  18. Jusnita N, Tridharma WS. Nannoemulsion characterization of horseradish Moringa oleifera Lamk. leaf extract. J Sains Farmasi Klin. 2019;6:16-24. https://doi.org/10.25077/jsfk.6.1.16-24.2019
  19. Kadarini T, Zamroni M, Pambayuningrum EK. Larval development of Rainbow Kurumoi (Melanotaenia parva) of hatchery. J Ris Akuakultur. 2013;8:77-86. https://doi.org/10.15578/jra.8.1.2013.77-86
  20. Khatoon H, Rahman NA, Banerjee S, Harun N, Suleiman SS, Zakaria NH, et al. Effects of different salinities and pH on the growth and proximate composition of Nannochloropsis sp. and Tetraselmis sp. isolated from South China Sea cultured under control and natural condition. Int Biodeterior Biodegrad. 2014;95:11-8. https://doi.org/10.1016/j.ibiod.2014.06.022
  21. Kumar P, Singh K, Kumar A. Hepatoprotective studies on aerial parts of Moringa oleifera Lam. on Carbon tetrachloride induced liver cell damage in albino rats. Ann Biol Res. 2010;1:27-35.
  22. Madigan MT, Martinko JM, Stahl DA, Clark DP. Brock biology of microorganisms. 13th ed. San Francisco, CA: Benjamin Cummings; 2010. p. 1152.
  23. Malakootian M, Hatami B, Dowlatshahi S, Rajabizadeh A. Growth and lipid accumulation in response to different cultivation temperatures in Nannochloropsis oculata for biodiesel production. Environ Health Eng Manag. 2016;3:29-34.
  24. Matos J, Cardoso C, Bandarra NM, Afonso C. Microalgae as healthy ingredients for functional food: a review. Food Funct. 2017;8:2672-85. https://doi.org/10.1039/C7FO00409E
  25. Misra A, Srivastava S, Srivastava M. Evaluation of anti diarrheal potential of Moringa oleifera (Lam.) leaves. J Pharmacogn Phytochem. 2014;2:43-6.
  26. Misra S, Misra MK. Nutritional evaluation of some leafy vegetable used by the tribal and rural people of south Odisha, India. J Nat Prod Plant Resour. 2014;4:23-8.
  27. Mufidah A, Agustono, Sudarno, Nindarwi DD. The culture technique of Chlorella sp. in laboratory scale and intermediates at the Balai Perikanan Budidaya Air Payau Situbondo East Java. J Aquacult Fish Health. 2017;7:50-6.
  28. Mukhlis A, Abidin Z, Rahman I. The effect of ammonium sulphate fertilizer concentration on the population growth of Nannochloropsis sp. J Biowallacea. 2018;3:149-55.
  29. Nurdiana S. Chlorella sp. cell density cultured at different light period. J Aquawarna. 2017;3:35-41.
  30. Nurfadillah DA, Adiwilaga EM. Community of phytoplankton in lake Laut Tawar, Aceh Tengah, Aceh province. Depik. 2012;1:93-8.
  31. Oluduro AO. Evaluation of antimicrobial properties and nutritional potentials of Moringa oleifera Lam. leaf in South-Western Nigeria. Malays J Microbiol. 2012;8:59-67.
  32. Purnamawat FS, Soeprobowati TR, Izzati M. The potential of Chlorella vulgaris in heavy metal Cd and Pb remediation in laboratory scale. Bioma: Berkala Ilmiah Biologi. 2014;16:102-13. https://doi.org/10.14710/bioma.16.2.102-113
  33. Qadariyah L, Mujaddid F, Bhuana DS, Mahfud M. Biodiesel production from microalgae with transesterification method using microwave. IOP Conf Ser Mater Sci Eng. 2018;543:012073.
  34. Qiu C, He Y, Huang Z, Li S, Huang J, Wang M, et al. Lipid extraction from wet Nannochloropsis biomass via enzyme-assisted three phase partitioning. Bioresour Technol. 2019;284:381-90. https://doi.org/10.1016/j.biortech.2019.03.148
  35. Rismiarti A, Kusumaningrum HP, Zainuri M. Characterization and molecular identification of Fusan derived from Chlorella pyrenoidosa and Chlorella vulgaris using 18SrDNA. Bioma: Berkala Ilmiah Biologi. 2016;18:30-40. https://doi.org/10.14710/bioma.18.2.30-40
  36. Sahira, Muskita WH, Astuti O. Effect of nitrophoska fertilizer dose on growth of Nannochloropsis sp. Media Akuatika. 2017;2:494-501.
  37. Sari IP, Manan A. Patterns growth of Nannochloropsis oculata in culture scale laboratory, intermediate, and bulk. J Ilm Perikanan Kelautan. 2012;4:123-7. https://doi.org/10.20473/jipk.v4i2.11562
  38. Selvika Z, Kusuma AB, Herliany NE, Negara BFSP. The growth rate of the Chlorella sp. at different concentrations of coal waste water. Depik. 2016;5:107-12.
  39. Sinaga L, Putriningtias A, Komariyah S. Influence of light intensity on the growth of Nannochloropsis sp. J Akuakultura. 2020;4:31-7.
  40. Toma A, Deyno S. Phytochemistry and pharmacological activities of Moringa oleifera. Int J Pharmacogn. 2014;1:222-31.
  41. Toripah SS, Abidjulu J, Wehantouw F. Antioxidant activity and total; phenolic content of horseradish Moringa oleifera Lamk leaf extract. J Pharmacon. 2014;3:37-43.
  42. Utami NF, Yuniarti MS, Haetami DK. Growth of Chlorella sp. cultured at different light periodicity. J Perikanan Kelautan Unpad. 2012;3:237-44.
  43. Wahyuni N, Rahardja BS, Azhar DMH. The effect of giving combination concentration of leaves of Moringa oleifera with Walne fertilizer in culture media on the growth and content of carotenoids in Dunaliella salina. J Aquac Sci. 2019;4:37-49. https://doi.org/10.31093/joas.v4i1.67
  44. Wu W, Logares R, Huang B, Hsieh CH. Abundant and rare picoeukaryotic sub-communities present contrasting patterns in the epipelagic waters of marginal seas in the northwestern Pacific Ocean. Environ Microbiol. 2017;19:287-300. https://doi.org/10.1111/1462-2920.13606
  45. Yameogo CW, Bengaly MD, Savadogo A, Nikiema PA, Traore SA. Determination of chemical composition and nutritional values of Moringa oleifera leaves. Pak J Nutr. 2011;10:264-8. https://doi.org/10.3923/pjn.2011.264.268
  46. Yuliani NN, Dienina DP. Antioxidant activity test of horseradish (Moringa oleifera Lamk) water extract using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. J Info Kesehatan. 2015;13:1061-82.
  47. Zaher SS, Helal AM. How culture medium pH range influence phytoplankton growth performance and biochemical content. Egypt J Aquat Biol Fish. 2020;24:103-16. https://doi.org/10.21608/ejabf.2020.111001
  48. Zongo U, Zoungrana SL, Savadogo A, Traore AS. Nutritional and clinical rehabilitation of severely malnourished children with Moringa oleifera Lam. leaf powder in Ouagadougou (Burkina Faso). Food Nutr Sci. 2013;4:991-7.
  49. Zuorro A, Maffei G, Lavecchia R. Optimization of enzyme-assisted lipid extraction from Nannochloropsis microalgae. J Taiwan Inst Chem Eng. 2016;67:106-14. https://doi.org/10.1016/j.jtice.2016.08.016