Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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A report of 30 unrecorded bacterial species in Korea, isolated from marine ecosystems in 2021

  • Shin, Seung Yeol (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Joung, Yochan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Han, Dukki (Department of Marine Molecular Bioscience, Gangneung-Wonju National University) ;
  • Jeong, Ji Hye (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Jeon, Yi Hyun (Division of Microbiology, Honam National Institute of Biological Resources) ;
  • Song, Jaeho (Division of Microbiology, Honam National Institute of Biological Resources)
  • Received : 2022.05.11
  • Accepted : 2022.06.30
  • Published : 2022.08.31
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Abstract

To obtain unrecorded bacterial species in Korea, various marine samples were collected from Jeollanam-do Province, Korea in 2021. After plating the samples on marine agar and marine R2A agar, and incubating aerobically and anaerobically, approximately 1200 bacterial strains were isolated and identified using 16S rRNA gene sequences. A total of 30 strains showed ≥98.7% 16S rRNA gene sequence similarity with validly published bacterial species but not reported in Korea, indicating that they are unrecorded bacterial species in Korea. The unrecorded bacterial strains belonged to 4 phyla, 7 classes, 13 orders, 19 families, and 22 genera, which were assigned to Azospirllium, Loktanella, and Pseudovibrio of the class Alphaproteobacteria; Grimontia, Halomonas, Marinobacter, Microbulbifer, Photobacterium, Pseudoalteromonas, Pseudidiomarina, Ferrimonas, Shewanella, Simiduia, Thalassotalea, and Vibrio of the class Gammaproteobacteria; Priestia and Enterococcus of the class Bacilli; Persicobacter of the class Cytophagia; Aureivirga of the class Flavobacteriia; Propionigenium and Psychrilyobacter of the class Fusobacteriia; and Tepidibacter of the class Clostridia. The details of the unreported species including Gram reaction, colony and cell morphology, biochemical characteristics, and phylogenetic position are also provided in the description of the strains.

Keywords

Acknowledgement

This study was supported by the research grant "Survey of Indigenous Species in Korean Islands" (HNIBR 202101111) from the Honam National Institute of Biological Resources of the Ministry of Environment in Korea.

References

  1. Beja, O., E.N. Spudich, J.L. Spudich, M. Leclerc and E.F. DeLong. 2001. Proteorhodopsin phototrophy in the ocean. Nature 411:786-789. https://doi.org/10.1038/35081051
  2. Carini, P., B.A. Van Mooy, J.C. Thrash, A. White, Y. Zhao, E.O. Campbell, H.F. Fredricks and S.J. Giovannoni. 2015. SAR11 lipid renovation in response to phosphate starvation. Proc Natl Acad Sci U S A, 112:7767-7772. https://doi.org/10.1073/pnas.1505034112
  3. Cho, J.C. and S.J. Giovannoni. 2004. Cultivation and growth characteristics of a diverse group of oligotrophic marine Gammaproteobacteria. Appl Environ Microbiol 70:432-440. https://doi.org/10.1128/AEM.70.1.432-440.2004
  4. Cho, J.C., K.L. Vergin, R.M. Morris and S.J. Giovannoni. 2004. Lentisphaera araneosa gen. nov., sp. nov, a transparent exopolymer producing marine bacterium, and the description of a novel bacterial phylum, Lentisphaerae. Environ Microbiol 6:611-621. https://doi.org/10.1111/j.1462-2920.2004.00614.x
  5. Cho, S.H., J.-H. Yoon, S.-B. Kim, K.-Y. Jahng, J.-C. Cho, K. Joh, C.-J. Cha, C.-N. Seong, J.-W. Bae, W.-T. Im and C.O. Jeon. 2017. A report of 29 unrecorded bacterial species belonging to the phylum Bacteroidetes in Korea. J Species Res 6:119-128.
  6. Chun, J., A. Oren, A. Ventosa, H. Christensen, D.R. Arahal, M.S. da Costa, A.P. Rooney, H. Yi, X.-W. Xu, S. De Meyer and M.E. Trujillo. 2018. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 68:461-466. https://doi.org/10.1099/ijsem.0.002516
  7. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.2307/2408678
  8. Giovannoni, S.J., L. Bibbs, J.C. Cho, M.D. Stapels, R. Desiderio, K.L. Vergin, M.S. Rappe, S. Laney, L.J. Wilhelm, H.J. Tripp. and E.J. Mathur. 2005. Proteorhodopsin in the ubiquitous marine bacterium SAR11. Nature 438:82-85. https://doi.org/10.1038/nature04032
  9. Joung, Y., C.-J. Cha, W.-T. Im, C.-O. Jeon, K. Joh, S.-B. Kim, W. Kim, S.D. Lee and J.-C. Cho. 2018. A report on 24 unrecorded bacterial species of Korea isolated in 2016, belonging to the orders Rhizobiales and Sphingomonadales in the class Alphaproteobacteria. J Species Res 7:13-23.
  10. Jung, H.S., J.-H. Yoon, K. Joh, C.-N. Seong, W.-Y. Kim, W.-T. Im, M.-K. Kim, C.-J. Cha, S.-B. Kim and C.-O. Jeon. 2021. A report of 35 unrecorded bacterial species belonging to the classes Alphaproteobacteria and Betaproteobacteria in Korea. J Species Res 10:12-22.
  11. Kumar, S., G. Stecher, M. Li, C. Knyaz and K. Tamura. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547-1549. https://doi.org/10.1093/molbev/msy096
  12. Sanchez-Baracaldo, P. 2015. Origin of marine planktonic cyanobacteria. Sci Rep 5:17418. https://doi.org/10.1038/srep17418
  13. Tripp, H.J. 2013. The unique metabolism of SAR11 aquatic bacteria. J Microbiol 51:147-153. https://doi.org/10.1007/s12275-013-2671-2
  14. Whitman, W.B., D.C. Coleman and W.J. Wiebe. 1998. Prokaryotes: the unseen majority. Proc Natl Acad Sci U S A. 95:6578-6583. https://doi.org/10.1073/pnas.95.12.6578
  15. Yang, S.-J. and J.-C. Cho. 2008. Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter. Int J Syst Evol Microbiol. 58:315318.
  16. Yoon, S.H., S.M. Ha, S. Kwon, J. Lim, Y. Kim, H. Seo and J. Chun. 2017. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613-1617. https://doi.org/10.1099/ijsem.0.001755