Mycobiology

The Korean Society of Mycology (한국균학회)
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Identification of Long Non-Coding RNAs and Their Target Genes from Mycelium and Primordium in Model Mushroom Schizophyllum commune

  • Tuheng Wu (Guangdong Yuewei Edible Fungi Technology Co.) ;
  • Jian Chen (Guangdong Yuewei Edible Fungi Technology Co.) ;
  • Chunwei Jiao (Guangdong Yuewei Edible Fungi Technology Co.) ;
  • Huiping Hu (Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences) ;
  • Qingping Wu (School of Bioscience and Bioengineering, South China University of Technology) ;
  • Yizhen Xie (Guangdong Yuewei Edible Fungi Technology Co.)
  • Received : 2022.04.20
  • Accepted : 2022.08.21
  • Published : 2022.10.31
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Abstract

Schizophyllum commune has emerged as the most promising model mushroom to study developmental stages (mycelium, primordium), which are two primary processes of fruit body development. Long non-coding RNA (lncRNA) has been proved to participate in fruit development and sex differentiation in fungi. However, potential lncRNAs have not been identified in S. commune from mycelium to primordium developmental stages. In this study, lncRNA-seq was performed in S. commune and 61.56 Gb clean data were generated from mycelium and primordium developmental stages. Furthermore, 191 lncRNAs had been obtained and a total of 49 lncRNAs were classified as differently expressed lncRNAs. Additionally, 26 up-regulated differently expressed lncRNAs and 23 down-regulated between mycelium and primordia libraries were detected. Further, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that differentially expressed lncRNAs target genes from the MAPK pathway, phosphatidylinositol signal, ubiquitin-mediated proteolysis, autophagy, and cell cycle. This study provides a new resource for further research on the relationship between lncRNA and two developmental stages (mycelium, primordium) in S. commune.

Keywords

Acknowledgement

We would like to thank Biomarker Technologies (Beijing, China) and Lijun Zhuo from the Institute of Microbiology, Guangdong Academy of Sciences for providing technological assistance.

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