Animal Bioscience

  • 제35권9호
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  • Pages.1444-1453
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  • 2022
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Mechanistic target of rapamycin and an extracellular signaling-regulated kinases 1 and 2 signaling participate in the process of acetate regulating lipid metabolism and hormone-sensitive lipase expression

  • Li, Yujuan (Department of Animal Science, Shandong Agricultural University) ;
  • Fu, Chunyan (Department of Animal Science, Shandong Agricultural University) ;
  • Liu, Lei (Department of Animal Science, Shandong Agricultural University) ;
  • Liu, Yongxu (Qingdao Kangda Food Co., LTD.) ;
  • Li, Fuchang (Department of Animal Science, Shandong Agricultural University)
  • 투고 : 2021.07.31
  • 심사 : 2021.10.01
  • 발행 : 2022.09.01
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초록

Objective: Acetate plays an important role in host lipid metabolism. However, the network of acetate-regulated lipid metabolism remains unclear. Previous studies show that mitogen-activated protein kinases (MAPKs) and mechanistic target of rapamycin (mTOR) play a crucial role in lipid metabolism. We hypothesize that acetate could affect MAPKs and/or mTOR signaling and then regulate lipid metabolism. The present study investigated whether any cross talk occurs among MAPKs, mTOR and acetate in regulating lipid metabolism. Methods: The ceramide C6 (an extracellular signaling-regulated kinases 1 and 2 [ERK1/2] activator) and MHY1485 (a mTOR activator) were used to treat rabbit adipose-derived stem cells (ADSCs) with or without acetate, respectively. Results: It indicated that acetate (9 mM) treatment for 48 h decreased the lipid deposition in rabbit ADSCs. Acetate treatment decreased significantly phosphorylated protein levels of ERK1/2 and mTOR but significantly increased mRNA level of hormone-sensitive lipase (HSL). Acetate treatment did not significantly alter the phosphorylated protein level of p38 MAPK and c-Jun aminoterminal kinase (JNK). Activation of ERK1/2 and mTOR by respective addition in media with ceramide C6 and MHY1485 significantly attenuated decreased lipid deposition and increased HSL expression caused by acetate. Conclusion: Our results suggest that ERK1/2 and mTOR signaling pathways are associated with acetate regulated HSL gene expression and lipid deposition.

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과제정보

This work was supported by the Natural Science Foundation of Shandong Province (ZR2021MC043), Natural Science Foundation of China (31972594), Special Economic Animal Industry Technology System of Shandong Province (SDAIT-21-16), Modern Agro-industry Technology Research system (CARS-43-B-1), Shandong Post-doctoral Innovation Project (2020), Taishan Industry Leadership Project (TSCY20190107), Key R & D projects of Xinjiang Autonomous Region (2020B01004-1-3).

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