Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Pretreatments of Broussonetia papyrifera: in vitro assessment on gas and methane production, fermentation characteristic, and methanogenic archaea profile

  • Dong, Lifeng (Feed Research Institute, Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/CAAS-CIAT Joint Laboratory in Advanced Technologies for Sustainable Agriculture) ;
  • Gao, Yanhua (College of Life Science and Technology, Southwest Minzu University) ;
  • Jing, Xuelan (Feed Research Institute, Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/CAAS-CIAT Joint Laboratory in Advanced Technologies for Sustainable Agriculture) ;
  • Guo, Huiping (College of Life Sciences, Henan Agricultural University) ;
  • Zhang, Hongsen (College of Life Sciences, Henan Agricultural University) ;
  • Lai, Qi (College of Life Science and Technology, Southwest Minzu University) ;
  • Diao, Qiyu (Feed Research Institute, Chinese Academy of Agricultural Sciences/Sino-US Joint Lab on Nutrition and Metabolism of Ruminant/CAAS-CIAT Joint Laboratory in Advanced Technologies for Sustainable Agriculture)
  • Received : 2020.07.20
  • Accepted : 2020.11.08
  • Published : 2022.09.01
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Abstract

Objective: The present study was conducted to examine the gas production, fermentation characteristics, nutrient degradation, and methanogenic community composition of a rumen fluid culture with Broussonetia papyrifera (B. papyrifera) subjected to ensiling or steam explosion (SE) pretreatment. Methods: Fresh B. papyrifera was collected and pretreated by ensiling or SE, which was then fermented with ruminal fluids as ensiled B. papyrifera group, steam-exploded B. papyrifera group, and untreated B. papyrifera group. The gas and methane production, fermentation characteristics, nutrient degradation, and methanogenic community were determined during the fermentation. Results: Cumulative methane production was significantly improved with SE pretreatment compared with ensiled or untreated biomass accompanied with more volatile fatty acids production. After 72 h incubation, SE and ensiling pretreatments decreased the acid detergent fiber contents by 39.4% and 22.9%, and neutral detergent fiber contents by 10.6% and 47.2%, respectively. Changes of methanogenic diversity and abundance of methanogenic archaea corresponded to the variations in fermentation pattern and methane production. Conclusion: Compared with ensiling pretreatment, SE can be a promising technique for the efficient utilization of B. papyrifera, which would contribute to sustainable livestock production systems.

Keywords

Acknowledgement

This study was funded by National Key R&D Program of China (2017YFF0211702), Key Program for International S&T Cooperation Projects of China (2016YFE0109000), National Natural Science Foundation of China (31802085, 20A180012), and Central Public-Interest Scientific Institution Basal Research Fund (Y2021GH18-2).

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