Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis

  • Yun, Hee Jung (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Jeoung, Da Jeoung (Department of Biopharmaceutics, Dong-eui University Graduate School) ;
  • Jin, Soojung (Core-Facility Center for Tissue Regeneration, Dong-eui University) ;
  • Park, Jung-ha (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Lee, Eun-Woo (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Lee, Hyun-Tai (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Choi, Yung Hyun (Core-Facility Center for Tissue Regeneration, Dong-eui University) ;
  • Kim, Byung Woo (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University) ;
  • Kwon, Hyun Ju (Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University)
  • Received : 2022.05.10
  • Accepted : 2022.06.29
  • Published : 2022.07.28
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Abstract

Proteins related to DNA replication have been proposed as cancer biomarkers and targets for anticancer agents. Among them, minichromosome maintenance (MCM) proteins, often overexpressed in various cancer cells, are recognized both as notable biomarkers for cancer diagnosis and as targets for cancer treatment. Here, we investigated the activity of cedrol, a single compound isolated from Juniperus chinensis, in reducing the expression of MCM proteins in human lung carcinoma A549 cells. Remarkably, cedrol also strongly inhibited the expression of all other MCM protein family members in A549 cells. Moreover, cedrol treatment reduced cell viability in A549 cells, accompanied by cell cycle arrest at the G1 phase, and enhanced apoptosis. Taken together, this study broadens our understanding of how cedrol executes its anticancer activity while demonstrating that cedrol has potential application in the treatment of human lung cancer as an inhibitor of MCM proteins.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant No. NRF-2017R1D1A1B04034994).

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