Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Comparison of the antioxidant and anti-inflammatory activities of leaf extracts from grain amaranths (Amaranthus spp.)

  • Ji, Hyo Seong (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Kim, Gayeon (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Ahn, Min-A (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Chung, Jong-Wook (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Hyun, Tae Kyung (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University)
  • Received : 2022.01.30
  • Accepted : 2022.03.23
  • Published : 2022.03.31
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Abstract

This study assessed the antioxidant and anti-inflammatory activities of leaf extracts from grain amaranths (Amaranthus spp.). Among all the extracts, the ethanol extract of Amaranthus cruentus leaves (Ar) exhibited the highest antioxidant activity, including the DPPH free radical scavenging activity and ORAC. In addition, Ar strongly inhibited nitric oxide production by suppressing the MEK/ERK signaling pathway in lipopolysaccharide-stimulated RAW264 murine macrophages. HPLC analysis revealed 13 polyphenolic compounds in the leaf extracts of grain amaranth and indicated that Ar contained more rutin than the other extracts. Taken together, these results show the impact of species diversity on the phytochemical contents and bioactivities of plant extracts and suggest that the nonedible parts, such as leaves, of A. cruentus should be considered for use as crude drugs and dietary health supplements.

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References

  1. Aldridge C, Razzak A, Babcock TA, Helton WS, Espat NJ (2008) Lipopolysaccharide-stimulated RAW 264.7 macrophage inducible nitric oxide synthase and nitric oxide production is decreased by an omega-3 fatty acid lipid emulsion. J Surg Res 149:296-302. https://doi.org/10.1016/j.jss.2007.12.758
  2. Asensio E, Vitales D, Perez I, Peralba L, Viruel J, Montaner C, Valles J, Garnatje T, Sales E (2020) Phenolic compounds content and genetic diversity at population level across the natural distribution range of bearberry (Arctostaphylos uva-ursi, ericaceae) in the Iberian Peninsula. Plants 9:1250. https://doi.org/10.3390/plants9091250
  3. Checa J, Aran JM (2020) Reactive oxygen species: drivers of physiological and pathological processes. J Inflamm Res 13:1057-1073. https://doi.org/10.2147/JIR.S275595
  4. Friedrich JC, Gonela A, Goncalves Vidigal MC, Vidigal Filho PS, Sturion JA, Cardozo Junior EL (2017) Genetic and phytochemical analysis to evaluate the diversity and relationships of mate (Ilex paraguariensis A.St.-Hil.) elite genetic resources in a germplasm collection. Chem Biodivers 14:e1600177. https://doi.org/10.1002/cbdv.201600177
  5. Frost RA, Nystrom GJ, Lang CH (2002) Lipopolysaccharide regulates proinflammatory cytokine expression in mouse myoblasts and skeletal muscle. Am J Physiol Regul Integr Comp Physiol 283:R698-709. https://doi.org/10.1152/ajpregu.00039.2002
  6. Gullon B, Lu-Chau TA, Moreira MT, Lema JM, Eibes G (2017) Rutin: A review on extraction, identification and purification methods, biological activities and approaches to enhance its bioavailability. Trends Food Sci Technol 67:220-235. https://doi.org/10.1016/j.tifs.2017.07.008
  7. Ju HJ, Kim KC, Kim H, Kim J-S, Hyun TK (2021) Variability of polyphenolic compounds and biological activities among Perilla frutescens var. crispa genotypes. Horticulturae 7:404. https://doi.org/10.3390/horticulturae7100404
  8. Karamac M, Gai F, Longato E, Meineri G, Janiak MA, Amarowicz R, Peiretti PG (2019) Antioxidant activity and phenolic composition of Amaranth (Amaranthus caudatus) during plant growth. Antioxidants 8:173. https://doi.org/10.3390/antiox8060173
  9. Li H, Deng Z, Liu R, Loewen S, Tsao R (2012) Ultra-performance liquid chromatographic separation of geometric isomers of carotenoids and antioxidant activities of 20 tomato cultivars and breeding lines. Food Chem 132:508-517. https://doi.org/10.1016/j.foodchem.2011.10.017
  10. Manyelo TG, Sebola NA, van Rensburg EJ, Mabelebele M (2020a) The probable use of genus amaranthus as feed material for monogastric animals. Animals 10:1504. https://doi.org/10.3390/ani10091504
  11. Manyelo TG, Sebola NA, Hassan ZM, Mabelebele M (2020b) Characterization of the phenolic compounds in different plant parts of Amaranthus cruentus grown under cultivated conditions. Molecules 25:4273. https://doi.org/10.3390/molecules25184273
  12. Manzoor Z, Koh Y-S (2012) Mitogen-activated protein kinases in inflammation. J Bacteriol Virol 42:189-195. https://doi.org/10.4167/jbv.2012.42.3.189
  13. Moniodis, J. Renton, M Jones, C G Barbour, E L Byrne, M (2018) Genetic and environmental parameters show associations with essential oil composition in West Australian sandalwood (Santalum spicatum). Aust J Bot 66:48-58. https://doi.org/10.1071/BT17116
  14. Olajide OA, Ogunleye BR, Erinle TO (2004) Anti-inflammatory properties of Amaranthus spinosus leaf extract. Pharm Biol 42:521-525. https://doi.org/10.1080/13880200490893285
  15. Pisarikova B, Zraly Z, Kracmar S, Trckova M, Herzig I (2006) The use of amaranth (genus Amaranthus L.) in the diets for broiler chickens. Vet Med 51:399-407. https://doi.org/10.17221/5560-VETMED
  16. Reyad-Ui-Ferdous Md, Shahjahan DMS, Tanvir S, Mukti M (2015) Present biological status of potential medicinal plant of Amaranthus viridis: a comprehensive review. Ame J Clin Exp Med 3:12-17. https://doi.org/10.11648/j.ajcem.s.2015030501.13
  17. Rodriguez-Yoldi MJ (2021) Anti-inflammatory and antioxidant properties of plant extracts. Antioxidants 10:921. https://doi.org/10.3390/antiox10060921
  18. Sarker U, Oba S (2019) Nutraceuticals, antioxidant pigments, and phytochemicals in the leaves of Amaranthus spinosus and Amaranthus viridis weedy species. Sci Rep 9:20413. https://doi.org/10.1038/s41598-019-50977-5
  19. Sarker U, Oba S (2020) Nutraceuticals, phytochemicals, and radical quenching ability of selected drought-tolerant advance lines of vegetable amaranth. BMC Plant Biol 20:564. https://doi.org/10.1186/s12870-020-02780-y
  20. Shakoor H, Feehan J, Apostolopoulos V, Platat C, Al Dhaheri AS, Ali HI, Ismail LC, Bosevski M, Stojanovska L (2021) Immunomodulatory effects of dietary polyphenols. Nutrients 13:728. https://doi.org/10.3390/nu13030728
  21. Yoo T-K, Jeong WT, Kim JG, Ji HS, Ahn M-A, Chung J-W, Lim HB, Hyun TK (2021) UPLC-ESI-Q-TOF-MS-based metabolite profiling, antioxidant and anti-inflammatory properties of different organ extracts of Abeliophyllum distichum. Antioxidants 10:70. https://doi.org/10.3390/antiox10010070
  22. Zhang DD, Zhang H, Lao YZ, Wu R, Xu JW, Murad F, Bian K, Xu HX (2015) Anti-inflammatory effect of 1,3,5,7-tetrahydroxy-8-isoprenylxanthone isolated from twigs of Garcinia esculenta on stimulated macrophage. Mediators Inflamm 2015:350564. https://doi.org/10.1155/2015/350564