Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory effects of Rosa rugosa extracts in RAW264.7 cells exposed to particulate matter (PM10)

미세먼지 PM10에 노출된 RAW264.7 세포에 대한 해당화 추출물의 항염증 활성

  • Ahn, Min-A (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University) ;
  • Hyun, Tae Kyung (Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University)
  • 안민아 (충북대학교 농업생명환경대학 특용식물학과) ;
  • 현태경 (충북대학교 농업생명환경대학 특용식물학과)
  • Received : 2022.06.07
  • Accepted : 2022.06.26
  • Published : 2022.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Airborne fine dust (FD) particles smaller than 10 ㎛ in diameter (PM10) are one of the major causes of air pollution in East Asia, including Korea, and have become a major contributor to respiratory and skin problems. FD inordinately promotes the production of reactive oxygen species and inflammatory response in macrophages, leading to cell damage and death. Rosa rugosa, a deciduous shrub of the Rosa genus, has been used in traditional East Asian herbal medicine to treat various illnesses. The present study investigated the anti-inflammatory effects of R. rugosa organ extracts on PM10-stimulated RAW264.7 macrophages. Compared to non-treated RAW264.7 cells, treatment with 100 ㎍.ml-1 PM10 resulted in increased nitric oxide (NO) production, similar to lipopolysaccharide treatment. Additionally, 100 ㎍/ml stem extract reduced NO production by more than 45% compared to mock treatment. Furthermore, PM10-induced expression of interleukin (IL)-1β, IL-6, inducible NO synthase, and cyclooxygenase-2 was significantly reduced by stem extract treatment, indicating that the anti-inflammatory effect of the stem extract is mediated by the inhibition of pro-inflammatory mediators in PM10-stimulated RAW 264.7 cells. These results indicate that the R. rugosa stem could be considered a natural remedy with a protective effect against inflammatory responses induced by harmful airborne dust.

직경이 10 ㎛ 미만의 미세먼지 입자(particulate matter 10, PM10)는 한국을 비롯한 동아시아 지역에서 해로운 대기오염의 주요한 원인 중 하나로 최근 주목받고 있으며, 호흡기와 피부에 유해한 영향을 미치는 주범이 되고 있다. FD는 대식세포에서 활성산소 종(ROS)의 생성과 염증반응을 과도하게 촉진시켜 세포 손상과 더불어 세포 사멸을 유도한다. 장미속의 낙엽관목인 해당화(Rosa rugosa)는 다양한 질병을 치료하는 목적으로 예부터 동아시아 지역에서 민간요법 치료제로써 사용되었다. 본 연구에서는 PM10으로 유도된 RAW 264.7 대식세포에서의 염증반응을 억제하는 해당화 각 기관별 추출물의 항염증 효과를 탐색하고자 한다. 미세먼지 입자가 처리되지 않은 RAW 264.7 대식세포와 비교했을 때, PM10을 100 ㎍/ml의 농도로 처리했을 경우 lipopolysaccharide 처리와 유사하게 nitric oxide (NO) 생성을 눈에 띄게 증가시켰다. 또한 100 ㎍/ml의 해당화 줄기 추출물은 미세먼지 입자 단독 처리군에 비해 NO 생성을 45% 이상 감소시켰고, PM10에 의해 유도된 interleukin(IL)-1β, IL-6, inducible nitric oxide synthase, and cyclooxygenase-2의 발현을 유의하게 감소시켰다. 이러한 결과는 해당화 줄기 추출물의 항염증 효과가 PM10으로 자극된 RAW 264.7 세포에서 염증 유발 매개체의 억제에 의해 매개됨을 나타내며, 해당화 줄기가 유해한 공기 중 미세먼지 입자에 의해 유발되는 염증반응에 대한 보호 효과가 있는 천연 자원으로서 가치가 있음을 시사한다.

Keywords

Acknowledgement

본 연구는 산업통상자원부 및 산업기술평가관리원(KEIT) 연구비지원에(과제번호: P0018148) 의해 수행되었다

References

  1. Calderon-Garciduenas L, Kavanaugh M, Block M, D'Angiulli A, Delgado-Chavez R, Torres-Jardon R, Gonzalez-Maciel A, Reynoso-Robles R, Osnaya N, Villarreal-Calderon R, Guo R, Hua Z, Zhu H, Perry G, Diaz P (2012). Neuroinflammation, hyperphosphorylated tau, diffuse amyloid plaques, and down-regulation of the cellular prion protein in air pollution exposed children and young adults. J Alzheimers Dis. 28:93-107 https://doi.org/10.3233/JAD-2011-110722
  2. Fernando IP, Kim HS, Sanjeewa KK, Oh JY, Jeon YJ, Lee WW (2017). Inhibition of inflammatory responses elicited by urban fine dust particles in keratinocytes and macrophages by diphlorethohydroxycarmalol isolated from a brown alga Ishige okamurae. Algae. 32:261-273 https://doi.org/10.4490/algae.2017.32.8.14
  3. Gasparrini M, Afrin S, Forbes-Hernandez TY, Cianciosi D, Reboredo-Rodriguez P, Amici A, Battino M, Giampieri F (2018). Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages. Part 2: Control of oxidative stress induced damage, increase of antioxidant enzyme activities and attenuation of inflammation. Food Chem Toxicol. 120:578-587 https://doi.org/10.1016/j.fct.2018.08.001
  4. Jung HJ, Nam JH, Choi J, Lee KT, Park HJ (2005). 19α-hydroxyursane-type triterpenoids: antinociceptive anti-inflammatory principles of the roots of Rosa rugosa. Biol Pharm Bull. 28:101-104 https://doi.org/10.1248/bpb.28.101
  5. Kang NS, Sohn EH (2010). Immunomodulatory effects of fructus and semen from Rosa rugosa on macrophages. Korean J Plant Res. 23: 399-405
  6. Kany S, Vollrath JT, Relja B (2019). Cytokines in Inflammatory Disease. Int J Mol Sci. 20:6008 https://doi.org/10.3390/ijms20236008
  7. Kim E, Mok HK, Hyun TK (2022). Variations in the antioxidant, anticancer, and anti-inflammatory properties of different Rosa rugosa organ extracts. Agronomy. 12:238 https://doi.org/10.3390/agronomy12020238
  8. Kim JW, Um M, Lee JW (2018). Antioxidant activities of hot water extracts from different parts of Rugosa rose (Rosa rugosa Thunb.). J Korean Wood Sci Technol. 46:38-47 https://doi.org/10.5658/WOOD.2018.46.1.38
  9. Kwak M, Eom SH, Gil J, Kim JS, Hyun TK (2019). Variation in bioactive principles and bioactive compounds of Rosa rugosa fruit during ripening. J Plant Biotechnol. 46:236-245 https://doi.org/10.5010/JPB.2019.46.3.236
  10. Lee JW, Seok JK, Boo YC (2018). Ecklonia cava extract and dieckol attenuate cellular lipid peroxidation in keratinocytes exposed to PM10. Evid Based Complement Alternat Med. 2018:8248323
  11. Park BJ (2008). Isolation of main component and antioxidant activities on the stem and root of Rosa rugosa. Korean J Plant Res. 21:402-407
  12. Park S, Seok JK, Kwak JY, Suh HJ, Kim YM, Boo YC (2016). Anti-inflammatory effects of pomegranate peel extract in THP-1 cells exposed to particulate matter PM10. Evid Based Complement Alternat Med. 2016:6836080
  13. Sharma JN, Al-Omran A, Parvathy SS (2007). Role of nitric oxide in inflammatory diseases. Inflammopharmacology. 15:252-259 https://doi.org/10.1007/s10787-007-0013-x
  14. Toriyabe M, Omote K, Kawamata T, Namiki A (2004). Contribution of interaction between nitric oxide and cyclooxygenases to the production of prostaglandins in carrageenan-induced inflammation. Anesthesiology. 101:983-990 https://doi.org/10.1097/00000542-200410000-00025
  15. Yoo TK, Jeong WT, Kim JG, Ji HS, Ahn MA, Chung JW, Lim HB, Hyun TK (2021). UPLC-ESI-Q-TOF-MS-Based metabolite profiling, antioxidant and anti-inflammatory properties of different organ extracts of Abeliophyllum distichum. Antioxidants. 10:70 https://doi.org/10.3390/antiox10010070
  16. Zhang DD, Zhang H, Lao YZ, Wu R, Xu JW, Murad F, Bian K, Xu HX (2015). Anti-inflammatory effect of 1, 3, 5, 7-tetrahydroxy-8-isoprenylxanthone isolated from twigs of Garcinia esculenta on stimulated macrophage. Mediators Inflamm. 2015:350564 https://doi.org/10.1155/2015/350564