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Taxonomic Status of Acheilognathus sp. HR (Cypriniformes: Acheilognathidae) Found in the Dalcheon River, a Tributary of Hangang River, Korea

한강 지류 달천에서 발견된 Acheilognathus sp. HR (Cypriniformes: Acheilognathidae)의 분류학적 위치

  • Yong Hwi Kim (Department of Biology, College of Natural Sciences, Soonchunhyang University) ;
  • Bong Han Yun (Department of Biology, College of Natural Sciences, Soonchunhyang University) ;
  • Mu Sung Sung (Department of Biology, College of Natural Sciences, Soonchunhyang University) ;
  • In-Chul Bang (Department of Biology, College of Natural Sciences, Soonchunhyang University)
  • 김용휘 (순천향대학교 생명과학과) ;
  • 윤봉한 (순천향대학교 생명과학과) ;
  • 성무성 (순천향대학교 생명과학과) ;
  • 방인철 (순천향대학교 생명과학과)
  • Received : 2022.09.01
  • Accepted : 2022.12.21
  • Published : 2022.12.31

Abstract

To investigate the taxonomic status of undescribed species Acheilognathus sp. HR from Korean Acheilognathidae discovered from the Dalcheon River, a tributary of the Hangang River, molecular phylogenetic and morphological characteristics were compared and analyzed with previous studies. As a result of molecular phylogenetic analysis, A. sp. HR formed the same genetic clade as the five subspecies of Acheilognathus tabira, but formed a separate monophyletic group based on the unique genotype, showing clear differences. As a result of morphological analyses, the dorsal fin color in males is grayish and the nuptial coloration of the outer edge of the anal fin is white. The outer edges of the dorsal and anal fins are convexly rounded. A black blotch is present on the dorsal fin of the juvenile, but there is a black blotch absent on the dorsal fin of the small adult female. In the counts, the number of branched dorsal rays is 12~13. In the measurements, the length of the barbels is short and the body depth is deep. Therefore, the A. sp. HR of Hangang River is considered at the level of a distinct species distinguished from each other by the five subspecies of A. tabira by molecular phylogenetic, morphological, and limited distributional characteristics.

한강 지류인 달천에서 발견된 한국산 납자루과 1미기재종 Acheilognathus sp. HR의 분류학적 위치를 알아보기 위하여 분자계통학적 및 형태학적 특성을 선행 연구와 비교 분석하였다. 분자계통학적 분석 결과에서 A. sp. HR은 Acheilognathus tabira의 5개 아종과 동일한 유전적 clade를 형성하면서도 고유한 유전자형을 기반으로 별도의 단계통군을 형성하여 A. tabira의 5개 아종과도 분명한 차이를 보였다. 또한, 형태학적 분석 결과에서 A. sp. HR은 수컷의 등지느러미와 뒷지느러미 외연부는 볼록하게 둥글고, 각각 회색과 흰색을 띠는 점, 치어의 등지느러미 상에는 흑색 반점이 존재하나 암컷 성어의 등지느러미 상에는 흑색 반점이 존재하지 않는 점, 등지느러미 분기 연조 수가 12~13개로 그 범위가 중복되지 않을 정도로 많은 점, 입수염 길이가 짧고, 체고가 높은 점 등에서 A. tabira를 구성하는 5개 아종과 잘 구분되었다. 따라서, A. sp. HR은 A. tabira의 5개 아종과 분자계 통학적, 형태학적 및 제한적 분포 특성에 의하여 서로 구별되는 별도의 종 수준에 있다고 판단된다.

Keywords

Acknowledgement

본 연구에서 일부 'Acheilognathus sp. HR' 표본 확보와 현지 채집에 도움을 주신 보령민물생태관의 조성장 관장님께 감사의 말씀을 전해 드리며, 낙동강 '가시납지리 Acheilognathus chankaensis' 표본을 제공해주신 국립낙동강생물자원관의 김상기 박사님께도 감사의 말씀을 전합니다. 이 논문은 순천향대학교의 연구비를 지원받아 수행된 연구입니다.

References

  1. Arai, R. and Y. Akai. 1988. Acheilognathus melnogaster, a senior synonym of A. moriokae, with a revision of the genera of the subfamily Acheilognathinae (Cypriniformes, Cyprinidae). Bull. Natn. Sci. Mus., 14: 199-213.
  2. Arai, R., H. Fujikawa and Y. Nagata. 2007. Four new subspecies of Acheilognathus bitterlings (Cyprinidae: Acheilognathinae) from Japan. Bull. Nat. Sci. Mus. Tokyo. Ser. A. Suppl., 1: 1-28.
  3. Bleeker, P. 1860. Ordo Cyprini, Karpers. Act. Soc. Sci. Indo-Neerl., 7: 1-492.
  4. Bleeker, P. 1871. Memoire sur les cyprinoides de Chine. Verh. Akad. Amsterdam, 12: 1-91. pls. 1-14.
  5. Chae, B.S., H.B. Song and J.Y. Park. 2019. A field guide to the freshwater fishes of Korea. LG Evergreen Foundation, Seoul, Korea, 355pp. (in Korean)
  6. Chae, B.S., S.K. Kim, J.H. Lee and U.W. Hwang. 2014. Taxonomic status of Acheilognathus sp. (Cyprinidae) found in the Geum River, Korea. Korean J. Ichthyol., 26: 249-258. (in Korean)
  7. Chang, C.H., F. Li, K.T. Shao, Y.S. Lin, T. Morosawa, S.M. Kim, H.Y. Koo, W. Kim, J.S. Lee, S. He, C. Smith, M. Reichard, M. Miya, T. Sado, K. Uehara, S. Lavoue, W.J. Chen and R.L. Mayden. 2014. Phylogenetic relationships of Acheilognathidae (Cypriniformes: Cyprinoidea) as revealed from evidence of both nuclear and mitochondrial gene sequence variation: evidence for necessary taxonomic revision in the family and the identification of cryptic species. Mol. Phylogenet. Evol., 81: 182-194. https://doi.org/10.1016/j.
  8. Choi, K.C. and S.R. Jeon. 1974. Survey on the fresh-water fish fauna aside from the DMZ. In: The reports on the scientific survey of near the DMZ. Bureau of Cultural Property Ministry of Culture and Information Republic of Korea. The report of the Korean association for conservation of nature, pp. 258-285. (in Korean)
  9. Chyung, M.K. 1954. Korean fishes. Department of Commerce and Industry, Republic of Korea, 14+1+48+517+12+16+12+4+13pp. (in Korean)
  10. Chyung, M.K. 1961. Illustrated Encyclopedia: The Fauna of Korea. 2. Fishes, Ministry of Education, Seoul, Korea, 861pp. (in Korean)
  11. Chyung, M.K. 1977. The fishes of Korea. Iljisa, Seoul, Korea, 727pp, 238pls. (in Korean)
  12. Darriba, D., G.L. Taboada, R. Doallo and D. Posada. 2012. jModel Test 2: more models, new heuristics and parallel computing. Nat. Methods, 9: 772. https://doi.org/10.1038/nmeth.2109.
  13. Dybowski, B.I. 1872. Zur Kenntniss der Fischfauna des Amurgebietes. Verh. K. K. Zool. Bot. Ges. Wien v. 22: 209-222.
  14. Fricke, R., W.N. Eschmeyer and J.D. Fong. 2022a. Eschmeyer's Catalog of Fishes: genera/species by family/subfamily. Available at: http://researcharchive.calacademy.org/research/ichthyology/catalog/SpeciesByFamily.asp (accessed 23 Augst 2022).
  15. Fricke, R., W.N. Eschmeyer and R. Van der Laan. 2022b. Eschmeyer's Catalog of Fishes: genera, species, references. Available at: http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp (accessed 23 August 2022).
  16. Guindon, S. and O. Gascuel. 2003. A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood". Syst. Biol., 52: 696-704. https://doi.org/10.1080/10635150390235520.
  17. Hosoya, K. 2015. Freshwater fishes of Japan. Yama-kei Publishers Co., Ltd., Tokyo, Japan, 527pp. (in Japanese)
  18. Jordan, D.S. and H.W. Fowler. 1903. A review of the cyprinoid fishes of Japan. Proc. U. S. Natl. Mus., 26: 811-862. https://doi.org/10.5479/si.00963801.26-1334.811.
  19. Jordan, D.S. and W.F. Thompson. 1914. Record of the fishes obtained in Japan in 1911. Mem. Carnegie Mus., 6: 205-313, 24-42pls. https://doi.org/10.5962/p.38099.
  20. Katoh, K., J. Rozewicki and K.D. Yamada. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief. Bioinformatics, 20: 1160-1166. https://doi.org/10.1093/bib/bbx108.
  21. Kawamura, K., T. Ueda, R. Arai and C. Smith. 2014. Phylogenetic relationships of bitterling fishes (Teleostei: Cypriniformes: Acheilognathinae), inferred from mitochondrial cytochrome b sequences. Zool. Sci., 31: 321-329. https://doi.org/10.2108/zs130233.
  22. Kim, I.S. and H. Yang. 1998. Acheilognathus majusculus, a new bitterling (Pisces, Cyprinidae) from Korea, with revised key to species of the genus Acheilognathus of Korea. Korean J. Biol. Sci., 2: 27-31. https://doi.org/10.1080/12265071.1998.9647387.
  23. Kim, D.M., H.B. Jeon and H.Y. Suk. 2014. Tanakia latimarginata, a new species of bitterling from the Nakdonggang River, South Korea (Teleostei: Cyprinidae). Ichthyol. Explor. Freshwaters, 25: 59-68.
  24. Kim, H.S. and I.S. Kim. 2009. Acanthorhodeus gracilis, a junior synonym of Acheilognathus chankaensis (Pisces: Cyprinidae) from Korea. Korean J. Ichthyol., 21: 55-60.
  25. Kim, I.S. 1997. Illustrated Encyclopedia of Fauna & Flora of Korea, Vol. 37, Freshwater Fishes. Ministry of Education, Yeongi, 518pp.(in Korean)
  26. Kim, I.S. and J.Y. Park. 2002. Freshwater fish of Korea. Kyohak Publishing Co., Seoul, Korea, 467pp. (in Korean)
  27. Kitamura, J., N. Nagata, J. Nakajima and T. Sota. 2012. Divergence of ovipositor length and egg shape in a brood parasitic bitterling fish through the use of different mussel hosts. J. Evol. Biol., 25: 566-573. https://doi.org/10.1111/j.1420-9101.2011.02453.x.
  28. Kuraku, S., C.M. Zmasek, O. Nishimura and K. Katoh. 2013. aLeaves facilitates on-demand exploration of metazoan gene family trees on MAFFT sequence alignment server with enhanced interactivity. Nucleic Acids Res., 41: W22-W28. https://doi.org/10.1093/nar/gkt389.
  29. Lanfear, R., P.B. Frandsen, A.M. Wright, T. Senfeld and B. Calcott. 2017. PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol. Biol. Evol., 34: 772-773. https://doi.org/10.1093/molbev/msw260.
  30. Li, F., T.Y. Liao, R. Arai and L. Zhao. 2017. Sinorhodeus microlepis, a new genus and species of bitterling from China (Teleostei: Cyprinidae: Acheilognathinae). Zootaxa, 4353: 69-88. https://doi.org/10.11646/zootaxa.4353.1.4.
  31. Mori, T. 1928a. A catalogue of the fishes of Korea. J. Pan-Pacific Res. Inst., 3: 3-8.
  32. Mori, T. 1928b. On the freshwater fishes from the Yalu River Korea, with descriptions of new species. J. Chosen Nat. Hist. Soc., 6: 54-70.
  33. Mori, T. 1935. Description of three new cyprinoids (Rhodina) from Chosen. Japan. Zool., 47: 559-574. (in Japanese)
  34. Nakamura, M. 1969. Cyprinid fish of Japan. Studies on the life history of cyprinid fishes of Japan. Res. Inst. For Natural Resources. Tokyo, Japan, 455pp. (in Japanese)
  35. Rambaut, A. 2018. FigTree. Version 1.4.4. Available at: http://tree.bio.ed.ac.uk/software/figtree (accessed 2 August 2022).
  36. Rambaut, A., A.J. Drummond, D. Xie, G. Baele and M.A. Suchard. 2018. Posterior summarisation in Bayesian phylogenetics using Tracer 1.7. Syst. Biol., 67: 901. https://doi.org/10.1093/sysbio/syy032.
  37. Ronquist, F. and J.P. Huelsenbeck. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19: 1572-1574. https://doi.org/10.1093/bioinformatics/btg180.
  38. Ronquist, F., M. Teslenko, P. van der Mark, D.L. Ayres, A. Darling, S. Hohna, B. Larget, L. Liu, M.A. Suchard and J.P. Huelsenbeck. 2012. MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Syst. Biol., 61: 539-542. https://doi.org/10.1093/sysbio/sys029.
  39. Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30: 1312-1313. https://doi.org/10.1093/bioinformatics/btu033.
  40. Temminck, C.J. and H. Schlegel. 1846. Pisces. In: Siebold, P.F. de (ed.), Fauna Japonica, sive descriptio animalium, quae in itinere per Japoniam suscepto annis 1823-1830 collegit, notis, observationibus et adumbrationibus illustravit Ph. Fr. de Siebold. Lugduni Batavorum [Leiden](A. Arnz et soc.). Parts 10-14, pp. 173-269. (in French) https://doi.org/10.5962/bhl.title.124951.
  41. Yang, Q., Y. Zhu, B. Xiong and H. Liu. 2011. Acheilognathus changtingensis sp. nov., a new species of the cyprinid genus Acheilognathus (Teleostei: Cyprinidae) from Southeastern China based on morphological and molecular evidence. Zool. Sci., 28: 158-167. https://doi.org/10.2108/zsj.28.158.