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Molecular Characterization of Filenchus cylindricus (Thorne & Malek, 1968) Niblack & Bernard, 1985 (Tylenchida: Tylenchidae) from Korea, with Comments on Its Morphology

  • Mwamula, Abraham Okki (Department of Ecological Science, Kyungpook National University) ;
  • Kim, Yiseul (Department of Ecological Science, Kyungpook National University) ;
  • Kim, Yeong Ho (Department of Ecological Science, Kyungpook National University) ;
  • Lee, Ho-wook (Department of Ecological Science, Kyungpook National University) ;
  • Kim, Young Ho (Department of Ecological Science, Kyungpook National University) ;
  • Lee, Dong Woon (Department of Ecological Science, Kyungpook National University)
  • Received : 2022.02.16
  • Accepted : 2022.06.08
  • Published : 2022.08.01

Abstract

Filenchus cylindricus (Thorne & Malek, 1968) Niblack & Bernard, 1985 was reported from the sandy rhizospheric soils of Poa pratensis and for the first time in Korea. Females and males are molecularly characterized and morphological and morphometric data supplied. Identification was made using an integrative approach considering morphological characteristics and inferences drawn from the analyses of the D2-D3 expansion segment of 28S rRNA and ITS1-5.8S-ITS2 of rRNA partial sequences. Females and males from Korea conform to the type descriptions and also to subsequent species descriptions from Iowa and Colorado USA, Sudan and Pakistan. Despite the close morphological and morphometric similarities with F. thornei (Andrássy, 1954) Andrássy, 1963, the two species can be adequately differentiated based on molecular data inference.

Keywords

Acknowledgement

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agriculture, Food and Rural Affairs Convergence Technologies Program for Educating Creative Global Leader Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA)(no.321001-03). The authors acknowledge Gapyeong Benest golf club superintendent and students from the turfgrass pests and nematology laboratory at Kyungpook National University for their assistance and cooperation during the survey.

References

  1. Andrassy, I. 1954. Revision der Gattung Tylenchus Bastian, 1865 (Tylenchidae, Nematoda). Acta Zool. 1:5-42.
  2. Andrassy, I. 1963. Freilebende Nematoden aus Angola, I. Einige moosbewohnende Nematoden. Publ. Cult. Comp. Diam. Angola Lisboa 66:57-79.
  3. Andrassy, I. 1988. Filenchus filipjevi sp. n. (Tylenchidae) a new soil inhabiting nematode species. Proc. Zool. Inst. Leningrad 180:25-29.
  4. Andrassy, I. 2007. Free-living nematodes of Hungary. II. Nematoda errantia. In: Pedozoologica Hungarica, No. 4, eds. by C. Csuzdi and S. Mahunka, pp. 1-496. Hungarian Natural History Museum, Budapest, Hungary.
  5. Atighi, M. R., Pourjam, E., Pereira, T. J., Okhovvat, S. M., Alizada, B. A., Mundo-Ocampo, M. and Baldwin, J. G. 2013. Redescription of Filenchus annulatus (Siddiqui & Khan, 1983) Siddiqi, 1986 based on specimens from Iran with contributions to the molecular phylogeny of the Tylenchidae. Nematology 15:129-141. https://doi.org/10.1163/156854112X649819
  6. Bai, M., Qing, X., Qiao, K., Ning, X., Xiao, S., Cheng, X. and Liu, G. 2020. Mitochondrial COI gene is valid to delimitate Tylenchidae (Nematoda: Tylenchomorpha) species. J. Nematol. 52:e2020-38.
  7. Bert, W., Leliaert, F., Vierstraete, A. R., Vanfleteren, J. R. and Borgonie, G. 2008. Molecular phylogeny of the Tylenchina and evolution of the female gonoduct (Nematoda: Rhabditida). Mol. Phylogenet. Evol. 48:728-744. https://doi.org/10.1016/j.ympev.2008.04.011
  8. Bert, W., Okada, H., Tavernier, I., Borgonie, G. and Houthoofd, W. 2010. Morphological, morphometrical and molecular characterisation of Filenchus fungivorus n. sp., a fungivorous nematode from Japan in a most likely polyphyletic genus (Nematoda: Tylenchina). Nematology 12:235-246. https://doi.org/10.1163/138855409x12470437876116
  9. Bongers, T. and Bongers, M. 1998. Functional diversity of nematodes. Appl. Soil Ecol. 10:239-251. https://doi.org/10.1016/S0929-1393(98)00123-1
  10. Brzeski, M. W. 1977. Lambertia uliginosa g. n., sp. n. (Nematoda, Nothotylenchidae). Nematol. Medit. 5:51-55.
  11. Brzeski, M. W. 1997. Redescription of some species of the genus Filenchus Andrassy, 1954 (Nematoda, Tylenchidae). Misc. Zool. 20:45-64.
  12. Brzeski, M. W. 1998. Nematodes of Tylenchina in Poland and temperate Europe. Muzeum i Instytut Zoologii Polska Akademia Nauk, Warszawa, Poland. 397 pp.
  13. Curran, J., Driver, F., Ballard, J. W. O. and Milner, R. J. 1994. Phylogeny of Metarhizium: analysis of ribosomal DNA sequence data. Mycol. Res. 98:547-552. https://doi.org/10.1016/s0953-7562(09)80478-4
  14. Darriba, D., Taboada, G. L., Doallo, R. and Posada, D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat. Methods 9:772.
  15. De Ley, P. and Blaxter, M. L. 2002. Systematic position and phylogeny. In: The biology of nematodes, ed. by D. L. Lee, pp. 1-30. Taylor & Francis, London, UK.
  16. Dobrin, I. and Geraert, E. 1994. Some tylenchida from Romania (Nematoda). Biol. Jaarb. Dodonaea. 62:121-133.
  17. Dobrin, I. and Rosca, I. 1996. Filenchus cylindricus and F. misellus, new species of nematodes from Romanian fauna. Rev. Roum. Biol. Ser. Biol. Anim. 41:103-107.
  18. Elmiligy, I. A. 1971. Two new species of Tylenchidae, Basiroides nortoni n. sp. and Tylenchus hageneri n. sp. (Nematoda: Tylenchida). J. Nematol. 3:108-112.
  19. Erdogus, F. D., Akgul, H. C. and Bayram, S. 2010. Four new record of species for Turkish nematode fauna: Filenchus cylindricus, F. sandneri, Lelenchus leptosoma and Geocenamus uralensis. Pak. J. Nematol. 28:241-251.
  20. Erum, Y. I. and Shahina, F. 2010. Taxonomic studies on parasitic and soil nematodes found associated with wheat in Pakistan. Pak. J. Nematol. 28:1-58.
  21. Ferris, H. and Bongers, T. 2006. Nematode indicators of organic enrichment. J. Nematol. 38:3-12.
  22. Fortuner, R., Louis, P.-Y. and Geniet, D. 2018. On the morphometric identity of populations of Helicotylenchus pseudoro-bustus (Steiner, 1914) Golden, 1956 (Tylenchida: Hoplolaimidae). Nematology 20:423-439. https://doi.org/10.1163/15685411-00003148
  23. Geraert, E. 2008. The Tylenchidae of the world: identification of the family Tylenchidae (Nematoda). Academia Press, Ghent, Belgium. 540 pp.
  24. Holterman, M., Karsen, G., Van Den Elsen, S., Van Megen, H., Baker, J. and Helder, J. 2008. SSU rDNA-based phylogeny of the Tylenchida sheds light on the evolution of plant feeding & establishes relationships among high impact parasitic nematodes. In: Holterman, M. 2008. Phylogenetic relationships within the phylum Nematoda as revealed by ribosomal DNA, and their biological implications. Ph.D. thesis, Wageningen University, Wageningen, The Netherlands. pp. 122-148.
  25. Husain, S. I. and Khan, A. M. 1965. A new genus and six new species of nematodes from India belonging in the family Neotylenchidae with an amendation of the subfamily Ecphyadophorinae. Proc. Helminthol. Soc. Wash. 32:7-15.
  26. Iwahori, H., Kanzaki, N. and Futai, K. 2000. A simple, polymerase chain reaction-restriction fragment length polymorphismaided diagnosis method for pine wilt disease. For. Pathol. 30:157-164. https://doi.org/10.1046/j.1439-0329.2000.00201.x
  27. Jenkins, W. R. 1964. A rapid centrifugal-floatation technique for separating nematodes from soil. Plant Dis. Rep. 48:692.
  28. Katalan-Gateva, S. and Tsoneva, P. 1977. Contribution to the study of the plant nematode fauna in Bulgaria. Godishnik Sofiiskiya Univ. Kliment Okhridski Biol. Fak. Kniga 1 Zool. 71:49-52.
  29. Lownsbery, J. W. and Lownsbery, B. F. 1985. Plant-parasitic nematodes associated with forest trees in California. Hilgardia 53:1-16. https://doi.org/10.3733/hilg.v53n05p016
  30. Mizukubo, T. and Minagawa, N. 1986. The genus Coslenchus Siddiqi, 1978 (Tylenchidae: Nematoda) from Japan. II. Synonymy of Coslenchus over Cosaglenchus and Paktylenchus based on the phylogenic relationships and a key to the species. Jpn. J. Nematol. 15:14-25.
  31. Mortazavi, P., Heydari, F., Abolafia, J., Castillo, P. and Pedram, M. 2021. Morphological and molecular characterization of Filenchus pseudodiscus n. sp. from east Golestan province, north Iran: with an updated phylogeny of Malenchus Andrassy, 1968 (Tylenchomorpha: Tylenchidae). J. Nematol. 53:e2021-69.
  32. Munawar, M., Castillo, P. and Yevtushenko, D. P. 2022. Description of Filenchus species from agroecosystem of Southern Alberta, Canada. Agronomy 12:690. https://doi.org/10.3390/agronomy12030690
  33. Munawar, M., Yevtushenko, D. P. and Castillo, P. 2021. First report of three Tylenchidae taxa from Southern Alberta, Canada. Horticulturae 7:449. https://doi.org/10.3390/horticulturae7110449
  34. Mwamula, A. O., Kabir, M. F., Lee, G., Choi, I. H., Kim, Y. H., Bae, E.-J. and Lee, D. W. 2020a. Morphological characterisation and molecular phylogeny of several species of Criconematina (Nematoda: Tylenchida) associated with turfgrass in Korea, as inferred from ribosomal and mitochondrial DNA. Nematology 22:939-956. https://doi.org/10.1163/15685411-bja10003
  35. Mwamula, A. O., Kim, Y. H., Na, H., An, H. J., Kim, Y. H. and Lee, D. W. 2020b. Molecular characterisation and phylogenetic position of Hemicycliophora labiata Colbran, 1960, from turfgrass in Korea, with comments on its morphology. Nematology 22:927-938. https://doi.org/10.1163/15685411-bja10002
  36. Mwamula, A. O., Lee, G. Kim, Y. H. Kim, Y. H. Lee, K. S. and Lee, D. W. 2021. Molecular phylogeny of several species of Hoplolaimina (Nematoda: Tylenchida) associated with turfgrass in Korea, with comments on their morphology. Nematology 23:559-576.
  37. Mwamula, A. O., Na, H., Kim, Y. H., Kim, Y. H., Han, G. and Lee, D. W. 2020c. Characterization of a new spiral nematode, Helicotylenchus asiaticus n. sp., and three known species from Korea: with comments on the validity of Helicotylenchus microlobus Perry in Perry, Darling & Thorne, 1959. Eur. J. Plant Pathol. 157:565-581. https://doi.org/10.1007/s10658-020-02022-9
  38. Niblack, T. L. and Bernard, E. C. 1985. Plant-parasitic nematode communities in dogwood, maple, and peach nurseries in Tennessee. J. Nematol. 17:132-139.
  39. Nunn, G. B. 1992. Nematode molecular evolution. Ph.D. thesis. University of Nottingham, Nottingham, UK.
  40. Okada, H., Harada, H. and Kadota, I. 2005. Fungal-feeding habits of six nematode isolates in the genus Filenchus. Soil Biol. Biochem. 37:1113-1120. https://doi.org/10.1016/j.soilbio.2004.11.010
  41. Orley, L. 1880. Monograph of the anguillulids. Termeszet. Fuzetek. 4:16-150 (in Magyar and German).
  42. Panahandeh, Y., Pourjam, E., Abolafia, J., Roshan-Bakhsh, A., Mojerlou, S., Afshar, F. J. and Pedram, M. 2019. Labrys khuzestanensis n. sp. (Nematoda, Tylenchidae), a new member of the genus with large labial plate. Zootaxa 4671:zootaxa.4671.2.7.
  43. Qing, X. and Bert, W. 2017. Redefinition of genus Malenchus Andrassy, 1968 (Tylenchomorpha: Tylenchidae) with additional data on ecology. J. Nematol. 49:189-206. https://doi.org/10.21307/jofnem-2017-064
  44. Qing, X. and Bert, W. 2019. Family Tylenchidae (Nematoda): an overview and perspectives. Org. Divers. Evol. 19:391-408. https://doi.org/10.1007/s13127-019-00404-4
  45. Qing, X., Decraemer, W., Claeys, M. and Bert, W. 2017. Molecular phylogeny of Malenchus and Filenchus (Nematoda: Tylenchidae). Zool. Scr. 46:625-636. https://doi.org/10.1111/zsc.12236
  46. Raski, D. J. and Geraert, E. 1987. Review of the genus Filenchus Andrassy, 1954 and descriptions of six new species (Nemata: Tylenchidae). Nematologica 32:265-311. https://doi.org/10.1163/187529286X00390
  47. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D. L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M. A. and Huelsenbeck, P. J. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61:539-542. https://doi.org/10.1093/sysbio/sys029
  48. Seinhorst, J. W. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4:67-69. https://doi.org/10.1163/187529259X00381
  49. Siddiqi, M. R. 1979. Seven new species in a new nematode subfamily Duosulciinae (Tylenchidae), with proposals for Duosulcius gen. n., Zanenchus gen. n. and Neomalenchus gen. n. Nematologica 25:215-236. https://doi.org/10.1163/187529279X00244
  50. Siddiqi, M. R. 1980. Two new nematode genera, Safianema (Anguinidae) and Discotylenchus (Tylenchidae), with descriptions of three new species. Proc. Helminthol. Soc. Wash. 47:85-94.
  51. Siddiqi, M. R. 1986. Tylenchida: parasites of plants and insects. Commonwealth Agricultural Bureaux, Farnham Royal, Slough, UK. 645 pp.
  52. Siddiqi, M. R. 2000. Tylenchida: parasites of plants and insects. CABI Bioscience, Egham, UK. 848 pp.
  53. Siddiqui, A. U. and Khan, E. 1983. Taxonomic studies on Tylenchidae (Nematoda) of India. II. Descriptions of two new sp. of Cosaglenghus gen. n. along with proposition of a new sub-family Aglenchinae. Indian J. Nematol. 12:303-311.
  54. Subbotin, S. A. Vovlas, N., Crozzoli, R., Sturhan, D., Lamberti, F., Moens, M. and Baldwin, J. G. 2005. Phylogeny of Criconematina Siddiqi, 1980 (Nematoda: Tylenchida) based on morphology and D2-D3 expansion segments of the 28S-rRNA gene sequences with application of a secondary structure model. Nematology 7:927-944. https://doi.org/10.1163/156854105776186307
  55. Swofford, D. L. 2003. PAUP*: phylogenetic analysis using parsimony and other methods. version 4.0a165. Sinauer Associates, Sunderland, MA, USA.
  56. Szczygiel, A. 1969. A new genus and four new species of the subfamily Tylenchinae de Man, 1876 (Nematoda: Tylenchidae) from Poland. Opusc. Zool. 9:159-170.
  57. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:4876-4882. https://doi.org/10.1093/nar/25.24.4876
  58. Thorne, G. and Malek, R. B. 1968. Nematodes of the Northern Great Plains. Part I. Tylenchida (Nemata: Secernentea). South Dakota Agric. Exp. Stn. Tech. Bull. 13:1-111.
  59. Wu, L.-Y. 1968. Dactylotylenchus crassacuticulus, a new genus and new species (Tylenchinae: Nematoda). Can. J. Zool. 46:831-834. https://doi.org/10.1139/z68-118
  60. Wu, L.-Y. 1969. Dactylotylenchinae, a new subfamily (Tylenchidae: Nematoda). Can. J. Zool. 47:909-911. https://doi.org/10.1139/z69-147
  61. Yeates, G. W. and Bird, A. F. 1994. Some observations on the influence of agricultural practices on the nematode faunae of some South Australian soils. Fundam. Appl. Nematol. 17:133-145.
  62. Zeidan, A. B. and Geraert, E. 1991. The genera Filenchus Andrassy, 1954, Sakia Khan, 1964, Boleodorus Thorne, 1941 and Basiria Siddiqi, 1959 (Nemata: Tylenchida) from Sudan. Nematologica 37:185-212. https://doi.org/10.1163/187529291X00187
  63. Zeng, Y., Ye, W., Tredway, L., Martin, S. and Martin, M. 2012. Taxonomy and morphology of plant-parasitic nematodes associated with turfgrasses in North and South Carolina, USA. Zootaxa 3452:1-46. https://doi.org/10.11646/zootaxa.3452.1.1