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Unrecorded Soil Fungi Isolated from the Dokdo, Korea

  • Eo, Ju-Kyeong (Division of Climate and Ecology, Bureau of Conservation & Assessment Research, National Institute of Ecology) ;
  • Park, Eunsu (Division of Climate and Ecology, Bureau of Conservation & Assessment Research, National Institute of Ecology) ;
  • Choi, Jae-Wook (Department of Biology Education, Korea National University of Education) ;
  • Shin, Hyun-Chul (Division of Climate and Ecology, Bureau of Conservation & Assessment Research, National Institute of Ecology) ;
  • Choi, Seung-Se (Division of Climate and Ecology, Bureau of Conservation & Assessment Research, National Institute of Ecology) ;
  • Park, So-Yeon (Division of Climate and Ecology, Bureau of Conservation & Assessment Research, National Institute of Ecology)
  • Received : 2021.06.30
  • Accepted : 2021.11.30
  • Published : 2022.08.01

Abstract

Two unrecorded fungi, Diaporthe perseae and Fusarium falciforme, were isolated from soil sampled from Dokdo in Korea. There have been many reports of Diaporthe sp. and Fusarium sp. in mainland Korea but none of them have reported in Dokdo so far. We used the morphological features and two molecular markers including the internal transcribed spacer and translation elongation factor 1-α region to compare and analyze these species with the closely related taxa. As a result, we confirmed that these fungi were unrecorded soil fungi in Korea. Then, the cultural and morphological characteristics such as the conidia of these two fungal species could be clarified. These results are expected to help us to understand the distribution of fungi in Dokdo and manage the Dokdo Island Natural Reserve.

Keywords

Acknowledgement

This study was supported with funds from the National Institute of Ecology under project No. NIE-C-2020-19, 2020 Ecosystem Survey of Dokdo Island, and the Ministry of Environment of Korea as a part of basic ecological research.

References

  1. Arnold, A.E. (2007). Understanding the diversity of foliar endophytic fungi: progress, challenges, and frontiers. Fungal Biology Reviews, 21, 51-66. https://doi.org/10.1016/j.fbr.2007.05.003
  2. Carrion, A. (1951). Cephalosporium falciforme sp. nov., a new etiologic agent of maduromycosis. Mycologia, 43, 522-523. https://doi.org/10.2307/3755425
  3. Cosoveanu, A., and Cabrera, R. (2018). Endophytic fungi in species of Artemisia. Journal of Fungi (Basel, Switzerland), 4, 53. https://doi.org/10.3390/jof4020053
  4. Das, K., Back, C.G., Lee, S.Y., and Jung, H.Y. (2018). Penicillifer diparietisporus: a new record from field soil in Korea. The Korean Journal of Mycology, 46, 227-233. https://doi.org/10.4489/KJM.20180029
  5. Dong, Z., Manawasinghe, I.S., Huang, Y., Shu, Y., Phillips, A., Dissanayake, A.J., et al. (2021). Endophytic Diaporthe associated with Citrus grandis cv. tomentosa in China. Frontiers in Microbiology, 11, 609387. https://doi.org/10.3389/fmicb.2020.609387
  6. Duarte, Y., Martinez, B., Santos do Carmo de Souza, E., and Cafe, A. (2019). First report of Fusarium falciforme affecting common bean (Phaseolus vulgaris) in Cuba. New Disease Reports, 40, 9. https://doi.org/10.5197/j.2044-0588.2019.040.009
  7. Eo, J.K., Park, H., Choi, S.S., Shin, H.C., Song, S.K., and Eom, A.H. (2017). Diversity of arbuscular mycorrhizal fungi isolated from Dokdo island. The Korean Journal of Mycology, 45, 284-291. https://doi.org/10.4489/KJM.20170036
  8. Gomes, R., Glienke, C., Videira, S., Lombard, L., Groenewald, J.Z., and Crous, P.W. (2013). Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia, 31, 1-41. https://doi.org/10.3767/003158513X666844
  9. Jeon, Y.G. (2005). The regional geomorphology of Dokdo (volcanic island). Journal of the Korean Association of Regional Geographers, 11, 19-28.
  10. Jo, J.W., Kwag, Y.N., Cho, S.E., Han, S.K., Han, J.G., Oh, S., et al. (2019). Xylodon flaviporus, a newly recorded macrofungi in Dokdo, South Korea. The Korean Journal of Mycology, 47, 241-247. https://doi.org/10.4489/KJM.20190028
  11. Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35, 1547-1549. https://doi.org/10.1093/molbev/msy096
  12. Lee, D.H., Cho, S.H., and Pak, J.H. (2007). The analysis of vascular plant species composition in Dok-do island. Korean Journal of Plant Taxonomy, 37, 545-563. https://doi.org/10.11110/kjpt.2007.37.4.545
  13. Lee, Y., Jung, H., Lee, H., Kim, S., Shin, K., Eom, A., et al. (2015). National List of Species of Korea: Ascomycota, Glomeromycota, Zygomycota, Myxomycota, Oomycota. Incheon: National Institute of Biological Resources.
  14. Lee, Y.S., Lim, Y.W., Kim, J.J., Yun, H.Y., Kim, C., and Park, J.Y. (2015). National List of Species of Korea: Basidiomycota. Incheon: National Institute of Biological Resources.
  15. Martinez-Salgado, M.M., Gutierrez-Romero, V., Jannsens, M., and Ortega-Blu, R. (2010). Biological soil quality indicators: a review. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 1, 319-328.
  16. Ministry of Environment, National Institute of Ecology. (2016). Ecosystem Survey of Dokdo Island. Seocheon: National Institute of Ecology.
  17. Munsell Color. (2012). The Munsell Book of Color: Glossy Collection. Grand Rapids: X-Rite.
  18. Nelson, P.E., Dignani, M.C., and Anaissie, E.J. (1994). Taxonomy, biology, and clinical aspects of Fusarium species. Clinical Microbiology Reviews, 7, 479-504. https://doi.org/10.1128/cmr.7.4.479-504.1994
  19. Park, S.J., Song, I.G., Park, S.J., and Lim, D.O. (2010). The flora and vegetation of Dokdo island in Ulleung-gun, Gyeongsanbuk-do. Korean Journal of Environment and Ecology, 24, 264-278.
  20. Petersen, J.H. (2013). The Kingdom of Fungi. Princeton: Princeton University Press.
  21. Rehner, S.A., and Buckley, E. (2005). A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia, 97, 84-98. https://doi.org/10.1080/15572536.2006.11832842
  22. Saikkonen, K., Faeth, S., Helander, M., and Sullivan, T. (1998). Fungal endophytes: a continuum of interactions with host plants. Annual Review of Ecology and Systematics, 29, 319-343. https://doi.org/10.1146/annurev.ecolsys.29.1.319
  23. Schulz, B., and Boyle, C. (2005). The endophytic continuum. Mycological Research, 109(Pt 6), 661-686. https://doi.org/10.1017/S095375620500273X
  24. Sikes, B.A., Cottenie, K., and Klironomos, J.N. (2009). Plant and fungal identity determines pathogen protection of plant roots by arbuscular mycorrhizas. Journal of Ecology, 97, 1274-1280. https://doi.org/10.1111/j.1365-2745.2009.01557.x
  25. Sonn, Y.K., Park, C.W., Zhang, Y.S., Hyun, B.K., Song, K.C., and Yoon, E.S. (2011). Characteristics of soils distributed on the "Dokdo" island in South Korea. Korean Journal of Soil Science and Fertilizer, 44, 187-193. https://doi.org/10.7745/KJSSF.2011.44.2.187
  26. Vega-Gutierrez, T.A., Lopez-Urquidez, G.A., Allende-Molar, R., Amarillas-Bueno, L.A., Romero-Gomez, S.J., and Lopez-Orona, C.A. (2019). Aggressiveness and molecular characterization of Fusarium spp. associated with foot rot and wilt in Tomato in Sinaloa, Mexico. 3 Biotech, 9, 276. https://doi.org/10.1007/s13205-019-1808-3
  27. Vilgalys, R., and Hester, M. (1990). Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology, 172, 4238-4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  28. Watkinson, S.C., Boddy, L., and Money, N.P. (2015). The Fungi, 3rd ed. Waltham: Academic Press.
  29. White, T.J., Bruns, T., Lee, S., and Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White (Eds.), PCR Protocols: A Guide to Methods and Applications (pp. 315-322). San Diego: Academic Press.
  30. Yuan, Z.L., Rao, L.B., Chen, Y.C., Zhang, C.L., and Wu, Y.G. (2011). From pattern to process: species and functional diversity in fungal endophytes of Abies beshanzuensis. Fungal Biology, 115, 197-213. https://doi.org/10.1016/j.funbio.2010.11.002
  31. Zerova, M. (1940). Some Phomopsis species new for the USSR. Botanichnii Zhurnal, 1, 305-312.