Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

  • Sun, Chuanxi (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Zhu, Tianyi (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Zhu, Yuwei (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Li, Bing (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Zhang, Jiaming (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Liu, Yixin (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Juan, Changning (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Yang, Shifa (Institute of Poultry Science, Shandong Academy of Agricultural Sciences) ;
  • Zhao, Zengcheng (Institute of Poultry Science, Shandong Academy of Agricultural Sciences) ;
  • Wan, Renzhong (College of Animal Science and Veterinary Medicine, Shandong Agricultural University) ;
  • Lin, Shuqian (Institute of Poultry Science, Shandong Academy of Agricultural Sciences) ;
  • Yin, Bin (Institute of Poultry Science, Shandong Academy of Agricultural Sciences)
  • Received : 2022.01.19
  • Accepted : 2022.04.05
  • Published : 2022.07.31
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Abstract

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were "protein processing in endoplasmic reticulum," "retinol metabolism," and "glycine, serine, and threonine metabolism." Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.

Keywords

Acknowledgement

The current research was supported by Innovation Ability Promotion Project for Technology-based Small and Medium Enterprise in Shandong Province (2021TSGC1271, 2021TSGC1275), Shandong Provincial Central Government Guides Local Technology Development Fund Projects (YDZX20203700003775).

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