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Comparison of the bovine blood gas parameters produced with three types of portable blood gas analyzers

  • Ro, Younghye (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Choi, Woojae (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Hong, Leegon (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Eunkyung (Farm Animal Clinical Training and Research Center, Institutes of Green-Bio Science and Technology, Seoul National University) ;
  • Choe, Eunhui (Farm Animal Clinical Training and Research Center, Institutes of Green-Bio Science and Technology, Seoul National University) ;
  • Kim, Danil (Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University)
  • 투고 : 2022.02.22
  • 심사 : 2022.06.17
  • 발행 : 2022.07.31

초록

Background: A definite diagnosis should be made in the bovine practice field, however, it was difficult to perform laboratory analysis immediately. Currently, three types of portable blood gas analyzers are available in Korea. Objectives: This study aimed to evaluate the correlations among these three analyzers. Methods: Seventy-two plasma samples from Holstein-Friesian cows were used for blood gas analysis, and three instruments (EDAN i15 Vet, VETSCAN i-STAT, and EPOC) were operated simultaneously. Moreover, plasma calcium levels were compared between these portable analyzers and blood chemistry device, which is usually used in a laboratory environment. Pearson analysis was performed to confirm the correlation of each parameter produced with the three instruments and blood chemistry analyzer. Results: As results, high correlation was observed in parameters of pH, pO2, potassium ion, ionized calcium, and glucose (p < 0.001, r > 0.7). In addition, pCO2 showed a moderate correlation among the three analyzers (p < 0.001, r > 0.5), and there was no correlation among all instruments for sodium ions. There was also a high correlation between ionized calcium from the three portable devices and total calcium from the biochemistry analyzer (p < 0.001, r > 0.9). Conclusions: In conclusion, there was a high correlation between results from the three different blood gas analyzers used in the bovine clinical field in Korea. Thus, a consistent diagnosis can be made even with different equipment if the operator is aware of the strengths and weaknesses of each piece of equipment and operates it properly.

키워드

과제정보

This study was partially supported by the Research Institute for Veterinary Science, Seoul National University.

참고문헌

  1. Chamberlin WG, Middleton JR, Spain JN, Johnson GC, Ellersieck MR, Pithua P. Subclinical hypocalcemia, plasma biochemical parameters, lipid metabolism, postpartum disease, and fertility in postparturient dairy cows. J Dairy Sci. 2013;96(11):7001-7013. https://doi.org/10.3168/jds.2013-6901
  2. Leno BM, Martens EM, Felippe MJB, Zanzalari KP, Lawrence JC, Overton TR. Short communication: relationship between methods for measurement of serum electrolytes and the relationship between ionized and total calcium and neutrophil oxidative burst activity in early postpartum dairy cows. J Dairy Sci. 2017;100(11):9285-9293. https://doi.org/10.3168/jds.2017-12971
  3. Martinez N, Sinedino LD, Bisinotto RS, Ribeiro ES, Gomes GC, Lima FS, et al. Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows. J Dairy Sci. 2014;97(2):874-887. https://doi.org/10.3168/jds.2013-7408
  4. Yilmaz O, Karapinar T. Evaluation of the i-STAT analyzer for determination of ionized calcium concentrations in bovine blood. Vet Clin Pathol. 2019;48(1):31-35. https://doi.org/10.1111/vcp.12705
  5. Kronqvist C, Emanuelson U, Sporndly R, Holtenius K. Effects of prepartum dietary calcium level on calcium and magnesium metabolism in periparturient dairy cows. J Dairy Sci. 2011;94(3):1365-1373. https://doi.org/10.3168/jds.2009-3025
  6. Reinhardt TA, Lippolis JD, McCluskey BJ, Goff JP, Horst RL. Prevalence of subclinical hypocalcemia in dairy herds. Vet J. 2011;188(1):122-124. https://doi.org/10.1016/j.tvjl.2010.03.025
  7. Goff JP. The monitoring, prevention, and treatment of milk fever and subclinical hypocalcemia in dairy cows. Vet J. 2008;176(1):50-57. https://doi.org/10.1016/j.tvjl.2007.12.020
  8. Dillane P, Krump L, Kennedy E, Sayers RG, Sayers GP. Determining the predictive capability of a Clinical Assessment Scoring Chart to differentiate severity of the clinical consequences of neonatal calf diarrhea relative to gold-standard blood gas analysis. PLoS One. 2020;15(4):e0230708. https://doi.org/10.1371/journal.pone.0230708
  9. Lee SH, Choi EW, Kim D. Relationship between the values of blood parameters and physical status in Korean native calves with diarrhea. J Vet Sci. 2020;21(2):e17. https://doi.org/10.4142/jvs.2020.21.e17
  10. Lee S, Ok S, Kwon H, Kim D. Arterial and venous blood gas, electrolytes, biochemical and hematological values in healthy Korean native calves. J Vet Clin. 2015;32(6):499-503. https://doi.org/10.17555/jvc.2015.12.32.6.499
  11. Sayers RG, Kennedy A, Krump L, Sayers GP, Kennedy E. An observational study using blood gas analysis to assess neonatal calf diarrhea and subsequent recovery with a European Commission-compliant oral electrolyte solution. J Dairy Sci. 2016;99(6):4647-4655. https://doi.org/10.3168/jds.2015-10600
  12. Karapinar T, Kaynar O, Hayirli A, Kom M. Evaluation of 4 point-of-care units for the determination of blood l-lactate concentration in cattle. J Vet Intern Med. 2013;27(6):1596-1603. https://doi.org/10.1111/jvim.12204
  13. Kirsch K, Detilleux J, Serteyn D, Sandersen C. Comparison of two portable clinical analyzers to one stationary analyzer for the determination of blood gas partial pressures and blood electrolyte concentrations in horses. PLoS One. 2019;14(2):e0211104. https://doi.org/10.1371/journal.pone.0211104
  14. Suzuki K, Kondo N, Takagi K, Nishikawa A, Murakami Y, Otsuka M, et al. Validation of the bovine blood calcium checker as a rapid and simple measuring tool for the ionized calcium concentration in cattle. J Vet Med Sci. 2021;83(5):767-774. https://doi.org/10.1292/jvms.21-0001
  15. Neves RC, Stokol T, Bach KD, McArt JAA. Method comparison and validation of a prototype device for measurement of ionized calcium concentrations cow-side against a point-of-care instrument and a benchtop blood-gas analyzer reference method. J Dairy Sci. 2018;101(2):1334-1343. https://doi.org/10.3168/jds.2017-13779
  16. Lam V, Dhaliwal SS, Mamo JC. Adjustment of ionized calcium concentration for serum pH is not a valid marker of calcium homeostasis: implications for identifying individuals at risk of calcium metabolic disorders. Ann Clin Biochem. 2013;50(Pt 3):224-229. https://doi.org/10.1177/0004563212473747
  17. Ro Y, Choi W, Park J, Choe E, Kim D. Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na2EDTA. J Vet Med Sci. 2020;82(7):962-966. https://doi.org/10.1292/jvms.20-0048
  18. Gokce G, Citil M, Gunes V, Atalan G. Effect of time delay and storage temperature on blood gas and acid-base values of bovine venous blood. Res Vet Sci. 2004;76(2):121-127. https://doi.org/10.1016/j.rvsc.2003.08.009