Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effect of daflon-500®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male rats

  • Olatunji, Aishat O. (Veterinary Pharmacology and Toxicology, University of Ilorin) ;
  • Ayo, Joseph O. (Veterinary Physiology, Ahmadu Bello University) ;
  • Suleiman, Mohammed M. (Veterinary Pharmacology and Toxicology, Ahmadu Bello University) ;
  • Ambali, Suleiman F. (Veterinary Pharmacology and Toxicology, University of Ilorin) ;
  • Shittu, Muftau (Veterinary Pharmacology and Toxicology, Ahmadu Bello University) ;
  • Akorede, Ganiu J. (Veterinary Pharmacology and Toxicology, University of Ilorin) ;
  • Raji, Lukman O. (Theriogenology and Production, University of Ilorin) ;
  • Atata, Jamila A. (Veterinary Pathology, University of Ilorin) ;
  • Biobaku, Khalid T. (Veterinary Pharmacology and Toxicology, University of Ilorin) ;
  • Azeez, Mistura O. (Veterinary Physiology and Biochemistry, University of Ilorin)
  • Received : 2021.01.02
  • Accepted : 2021.11.30
  • Published : 2022.07.15
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Abstract

Alteration of redox status is one of the molecular pathways commonly associated with pesticide toxicity. Antioxidants, including those obtained from plant phenolics, have been shown to mitigate pesticide-induced cellular injury. The present study was aimed at evaluating the effect of dafon-500®, a flavonoid compound on sub-chronic chlorpyriphos-evoked changes in antioxidant and biochemical parameters in the hypophysis and testes of adult male rats. Twenty-five male albino rats were randomly divided into 5 groups of 5 animals each. Group I (DW) received distilled water (2 ml/kg); group II (SO) was dosed with soya oil (2 ml/kg); Group III (DAF) received dafon-500® at 1000 mg/kg~1/5th of LD50 (≥5000 mg/kg); group IV (CP) was administered chlorpyriphos at 7.74 mg/kg~1/10th of LD50 (77.4 mg/kg) while group V (DAF+CP) was previously treated with dafon-500® (1000 mg/kg) and then exposed to CP (7.74 mg/kg), 30 min later. Daily oral regimen administration was done for 60 days after which the animals were sacrificed by cervical venesection after light chloroform anesthesia. The hypophysis and testicular tissues were harvested, and their homogenates were analyzed for malondialdehyde, catalase and superoxide dismutase, and acetylcholinesterase levels. A significant increase in the hypophysis and testicular MDA concentrations, coupled with a decrease in the SOD, CAT, and AChE activities were observed in the CP group. The levels of these oxidative and biochemical parameters were alleviated in the group pretreated with Dafon-500®. Results of this study demonstrated that pre-treatment with Dafon-500® mitigated CP-induced alterations in oxidative and biochemical parameters apparently due to the antioxidant effect of the flavonoid compound.

Keywords

Acknowledgement

We acknowledged the staff of the Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria especially Mr. D. Otie for his laboratory expertise during the period of this study.

References

  1. Siegel JS (2012) The demography and epidemiology of human health and aging. Reprod Health 2:469-531
  2. Kim J, Shin DH, Lee WJ (2014) Suicidal ideation and occupational pesticide exposure among male farmers. Environ Res 128:52-56. https://doi.org/10.1016/j.envres.2013.10.007
  3. Neghab M, Momenbella-Fard M, Naziaghdam R et al (2014) The effects of exposure to pesticides on the fecundity status of farm workers resident in rural region of Fars province, southern Iran. Asian Pac J Trop Biomed 4:324-328. https://doi.org/10.12980/APJTB.4.2014C586
  4. Zhu J (2015) Advances in pesticide use in the cocoa belts and perceptions of vegetable farmers. J Hortic 2:149-152. https://doi.org/10.4172/2376-0354.1000149
  5. Oates L, Cohen M, Braun L et al (2014) Reduction in urinary organophosphate pesticides metabolites in adults after a weeklong organic diet. Environ Res 132:105-111. https://doi.org/10. 1016/j.envres.2014.03.021 https://doi.org/10.1016/j.envres.2014.03.021
  6. Ambali SF, Ayo JO, Ojo SA et al (2012) Chronic chlorpyrifos-induced sensorimotor and cognitive deficit in Wistar rats-reparation by Vitamin C. J Res Environ Sci Toxicol 1:221-232
  7. Joshi CS, Mathur R, Gulati N (2007) Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rats. Toxicol Ind Health 23:439-444. https://doi.org/10.1177/0748233707080908
  8. Shittu M, Olatunji OA, Ambali SF et al (2014) Ameliorative effect of Hibiscus sabdariffa Linn on subchronic chlorpyrifos-induced alteration in sex and thyroid hormones in male Wistar rats. Am J Pharmacol Toxicol 9:96-106. https://doi.org/10.3844/ajptsp.2014.96.106
  9. Ambali SF, Ayo JO (2011) Sensorimotor performance deficits induced by chronic chlorpyrifos exposure in Wistar rats: mitigative effect of vitamin C. Toxicol Environ Chem 93:1212-1226. https://doi.org/10.1080/02772248.2011.585991
  10. Ventura C, Venturino A, Miret N et al (2015) Chlorpyrifos inhibits cell proliferation through ERK1/2 phosphorylation in breast cancer lines. Chemosphere 120:343-350. https://doi.org/10.1016/j.chemosphere.2014.07.088
  11. Beam J, Botta A, Barendregt R et al (2014) Dietary fatty acids, redox signaling, and the heart. In: Laher I (ed) Systems biology of free radicals and antioxidants, part 111. Springer Verlag, Germany, pp 1497-1522
  12. Surai PF, Fisinin VI (2014) Antioxidant systems of the body: From vitamin E to polyphenols and beyond. In: Proceedings of the 35th Western Nutrition Conference. Edmonton, Canada, 24th-25th September 2014, pp 265-277
  13. Ramelet AA (2001) Clinical benefits of daflon 500 mg in the most severe stages of chronic venous insufficiency. Angiology 52:49-56. https://doi.org/10.1177/0003319701052001S07
  14. Rizk SM, Sabri NA (2009) Evaluation of clinical activity and safety of daflon 500mg in type 2 diabetic female patients. Saudi Pharm J 17:199-207. https://doi.org/10.1016/j.jsps.2009.08.008
  15. Canadian Council on Animal Care Guide (CACC) (1993) Canadian Council on Animal Care Guide (CACC), 2nd edn
  16. Olatunji AO (2017) Ameliorative effects of dafon-500® on subchronic chlorpyrifos - induced biochemical and reproductive changes in male albino rats. M.Sc. Dissertation, Ahmadu Bello University, Zaria
  17. Draper HH, Hardley M (1990) Malondialdehyde deformation as an index of lipid peroxidation. Methods Enzymol 186:421-431. https://doi.org/10.1016/0076-6879(90)86135-I
  18. Martin JP, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on haematoxylin autoxidation. Arch Biochem Biophys 225:329-336. https://doi.org/10.1016/0003-9861(87)90400-0
  19. Abei H (1974) Catalase. Methods in enzymatic analysis. Academic Press, New York, pp 673-684
  20. Ellman GL, Courtney KD, Anders V et al (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  21. Luna GH (1960) Manual of histologic staining method of armed forces institute of pathology, 35th edn. McGraw-Hill Book Company, New York, p 46
  22. El-bendary HM, Saleh AA, Negm SA et al (2014) Spermatogenic alterations induced by organophosphorus compounds profenofos, chlorpyrifos and synthetic pyrethroid lambada-cyhalothrin in mice. Annu Res Rev Biol 4:856-873. https://doi.org/10.9734/ARRB/2014/4925
  23. Heikal TM, Abdel-Tawab H, Mossa AW et al (2014) Oxidative damage and reproductive toxicity associated with cyromazine and chlorpyrifos in male rats: the protective effects of green tea extract. Res J Environ Toxicol 8:53-67. https://doi.org/10.3923/rjet.2014.53.67
  24. Gawish AM (2010) The protective role of alpha lipoic acid against pesticide induced testicular toxicity-histopathological and histochemical studies. J Aquac Res Development 1:1-7. https://doi.org/10.4172/2155-9546.1000101
  25. Gupta RC (2006) Classification and uses of organophosphates and carbamates. In: Gupta RC (ed) Toxicology of organophosphate and carbamate compounds, 1st edn. Academic Press (Elsevier), San Diego, pp 5-24
  26. Mandal TK, Das NS (2011) Correlation of testicular toxicity and oxidative stress induced by chlorpyrifos in rats. Hum Exp Toxicol 30:1529-1539. https://doi.org/10.1177/0960327110392400
  27. Salman KA, Ashraf S (2013) Reactive oxygen species: a link between chronic inflammation and cancer. Asia Pac J Mol Biol Biotechnol 21:42-49
  28. Slotkin TA (2004) Cholinergic systems in the brain development and disruption by neurotoxins: nicotine, environmental tobacco smoke, organophosphates. Toxicol Appl Pharmacol 198:132-151. https://doi.org/10.1016/j.taap.2003.06.001
  29. Molochkina EM, Zorina OM, Fatkullina LD (2005) H202 modifies membrane structure and activity of acetylcholinesterase. Chem Biol Interact 57:401-404. https://doi.org/10.1016/j.cbi.2005.10.075
  30. Rodriguez-Fuentes G, Rubio-Escalante FJ, Norena-Barroso E et al (2015) Impacts of oxidative stress on acetylcholinesterase transcription, and activity in embryos of zebrafish (Danio rerio) following Chlorpyrifos exposure. Comp Biochem Physiol 173:19-25. https://doi.org/10.1016/j.cbpc.2015.04.003
  31. Ross JA, Kasum CM (2002) Dietary flavonoids: bioavailability, metabolic effects, and safety. Annu Rev Nutri 22:19-34. https://doi.org/10.1146/annurev.nutr.22.111401.144957
  32. Khedr NF (2015) Protective effect of mirtazapine and hesperidin on cyclophosphamide-induced oxidative damage and infertility in rat ovaries. Exp Biol Med (Maywood) 240:1682-1689. https://doi.org/10.1177/1535370215576304
  33. Tamilselvam K, Nataraj J, Janakiraman U et al (2013) Antioxidant and anti-inflammatory potential of hesperidin against 1-methyl4-phenyl-1, 2, 3, 6-tetrahydropyridine-induced experimental Parkinson's disease in mice. Int J Nutr Pharm Neurol Dis 3:294-302. https://doi.org/10.4103/2231-0738.114875
  34. Roohbakhsh A, Parhiz H, Soltani F et al (2015) Molecular mechanisms behind the biological effects of hesperidin and hesperetin for the prevention of cancer and cardiovascular diseases. Life Sci 124:64-74. https://doi.org/10.1016/j.lfs.2014.12.030
  35. Silambarasan T, Raja B (2012) Diosmin, a biofavonoid reverses alterations in blood pressure, nitric oxide, lipid peroxides and antioxidant status in DOCA-salt induced hypertensive rats. Eur J Pharmacol 679:81-89. https://doi.org/10.1016/j.ejphar.2011.12.040