Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Hwangryunagyo-tang on Sleep Onset and Total Duration of Sleep Induced by Thiopental Dodium in Mice

Thiopental sodium으로 유도된 수면에서 황련아교탕의 입면 및 수면지속 효과

  • 임강현 (세명대학교 한의과대학) ;
  • 김태연 (세명대학교 한의과대학)
  • Received : 2021.12.29
  • Accepted : 2022.02.25
  • Published : 2022.02.25
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Abstract

Hwangryunagyo-tang (HT) is a herbal cocktail to treat insomnia induced by yin deficiency with effulgent fire. In the present study, the onset time and the duration time of sleep were measured 30 minutes after thiopental sodium injection. And c-fos immunohistochemistry was performed to find the activated nerve cells of the ventrolateral preoptic nucleus (VLPO) and tuberomammillary nucleus (TMN) sites in the brain. HT significantly increased the number of activated nerve cells in the sleep-inducing center (VLPO), whereas HT significantly decreased the number of activated nerve cells in the arousal center (TMN). It could be concluded that the HT shortened the onset time and increased the duration time for sleep induced by thiopental sodium. And it was confirmed that the mechanism acted by activating the sleep-inducing center (VLPO) and suppressing the arousal center (TMN) in the brain. The results are considered to be useful as scientific evidence HT can be used clinically for the treatment of insomnia caused by yin deficiency with effulgent fire.

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Acknowledgement

이 논문은 2021학년도 세명대학교 교내학술연구비 지원에 의해 수행된 연구임

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