Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Chronic Treatment of Taegeuk Ginseng on Cognitive Function Improvement in Scopolamine Induced Memory Retarded Rats

태극삼의 장기투여가 인지기능향상과 기억력증진에 미치는 영향

  • 이철형 (원광대학교 뇌과학연구소) ;
  • 박지혜 (원광대학교 뇌과학연구소) ;
  • 김규일 ((재)진안홍삼연구소) ;
  • 이서울 (원광대학교 뇌과학연구소)
  • Received : 2021.10.29
  • Accepted : 2022.02.11
  • Published : 2022.02.25
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Abstract

To investigate effects of cognitive function improvement whether against Taegeuk ginseng on scopolamine-induced memory impairment in rats. All experiments were conducted in three groups: the control group (CTR), the scopolamine 0.4mg/kg (SCP), and the scopolamine (SCP+T) treated with Taegeuk ginseng 100 mg/kg. Taegeuk ginseng 100 mg/kg daily was orally administered for one month and treated with scopolamine was only for 7 consecutive days on the Morris water maze task. 3 weeks after oral administration of Taegeuk ginseng, subjects were performed the Morris water maze test for 8 days and then the open-field exploration test which to assessed for cognitive function improvement. After behavioral testing, subjects were sacrificed and microdissected brains for neurochemical analysis. In the cognitive-behavioral test, long-term administration of Taegeuk ginseng improved spatial navigation learning task compared with the impeded by scopolamine treatment. In neurochemistry, the expression of the synaptic marker PSD95 (postsynaptic density protein 95) was increased in the hippocampus compared to the scopolamine group. Also, brain-derived neurotrophic factor (BDNF) expression was significantly increased in the taegeuk ginseng administration group. These data suggested that long-term administration of taegeuk ginseng might improve cognitive-behavioral functions on hippocampal related spatial learning memory, and it was correlated with neurotropic and synaptic reinforcement. In conclusion, treatment with taegeuk ginseng may positive outcome on learning and memory deficit disorders.

Keywords

Acknowledgement

본 연구는 한국연구재단의 지원을 받았음. (NRF-2020R1I1A3072383)

References

  1. Korean dementia observatory 2020, National Institute of Dementia, 2020.
  2. TUCKER-DROB, Elliot M. Cognitive aging and dementia: a life-span perspective. Annual review of developmental psychology, 2019;1:177-96. https://doi.org/10.1146/annurev-devpsych-121318-085204
  3. Szakaly Z, Szente V, Kover G, Polereczki Z, Szigeti O. The influence of lifestyle on health behavior and preference for functional foods. Appetite. 2012;58(1):406-13. https://doi.org/10.1016/j.appet.2011.11.003
  4. Korea Rural Economic Institute Policy Research Report, Korea Rural Economic Institute, 2019;7:1-115.
  5. Baek NI, Kim DS, Lee YH, Park JD, Lee CB, Kim SI. Ginsenoside Rh4, a genuine dammarane glycoside from Korean red ginseng. Planta Med. 1996;62(1):86-7. https://doi.org/10.1055/s-2006-957816
  6. Xiang YZ, Shang HC, Gao XM, Zhang BL. A comparison of the ancient use of ginseng in traditional Chinese medicine with modern pharmacological experiments and clinical trials. Phytother Res. 2008;22(7):851-8. https://doi.org/10.1002/ptr.2384
  7. Wang LC, Wang B, Ng SY, Lee TF. Effects of ginseng saponins on beta-amyloid-induced amnesia in rats. J Ethnopharmacol. 2006;103(1):103-8. https://doi.org/10.1016/j.jep.2005.07.010
  8. Jin SH, Park JK, Nam KY, Park SN, Jung NP. Korean red ginseng saponins with low ratios of protopanaxadiol and protopanaxatriol saponin improve scopolam-ineinduced learning disability and spatial working memory in mice. J Ethnopharmacol. 1999;66(2):123-9. https://doi.org/10.1016/S0378-8741(98)00190-1
  9. Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods. 1984;11(1):47-60. https://doi.org/10.1016/0165-0270(84)90007-4
  10. Yoon JJ. Korea's Aging and Medical Industry. The Korean Institute of Electrical Engineers. 2018;67(1):34-8.
  11. Lee CN, Park KW. Risk Factors of Dementia. Journal of Korean Diabetes. 2012;13(3):129-32. https://doi.org/10.4093/jkd.2012.13.3.129
  12. Gao S, Hendrie HC, Hall KS, Hui S. The relationships between age, sex, and the incidence of dementia and Alzheimer disease: a meta-analysis. Arch Gen Psychiatry. 1998;55(9):809-15. https://doi.org/10.1001/archpsyc.55.9.809
  13. Mitchell AJ, Kemp S, Benito-Le'on J, Reuber M. The influence of cognitive impairment on health-related quality of life in neurological disease. Acta Neuropsychiatrica. 2010;22(1):2-13. https://doi.org/10.1111/j.1601-5215.2009.00439.x
  14. Ali TB, Schleret TR, Reilly BM, Chen WY, Abagyan R. Adverse Effects of Cholinesterase Inhibitors in Dementia, According to the Pharmacovigilance Databases of the United-States and Canada. PLoS One. 2015;10(12):e0144337. https://doi.org/10.1371/journal.pone.0144337
  15. Matsunaga S, Kishi T, Iwata N. Memantine monotherapy for Alzheimer's disease: a systematic review and meta-analysis. PLoS One. 2015;10(4):e0123289. https://doi.org/10.1371/journal.pone.0123289
  16. Nam KY. Clinical Applications and Efficacy of Korean Ginseng. Journal of Ginseng Research, 2002;26(3):111-31. https://doi.org/10.5142/JGR.2002.26.3.111
  17. Zhang JT, Qu ZW, Liu Y, Deng HL. Preliminary study on antiamnestic mechanism of ginsenoside Rg1 and Rb1. Chin Med J (Engl). 1990;103(11):932-8.
  18. Yamaguchi Y, Haruta K, Kobayashi H. Effects of ginsenosides on impaired performance induced in the rat by scopolamine in a radial-arm maze. Psychoneuroendocrinology. 1995;20(6):645-53. https://doi.org/10.1016/0306-4530(95)00008-C
  19. KENNEDY DO, REAY JL, SCHOLEY AB. Effects of 8 weeks administration of Korean Panax ginseng extract on the mood and cognitive performance of healthy individuals. Journal of Ginseng Research. 2007;31(1):34. https://doi.org/10.5142/JGR.2007.31.1.034
  20. Jeong HC, Hong HD, Kim YC, Rho J, Kim KT, Cho CW. The research trend of ginseng processing technology and the status of ginseng industry. Food Science and Industry. 2012;45(4):59-67. https://doi.org/10.23093/FSI.2012.45.4.59
  21. Kim ST, Jang JH, Kwon JH, Moon KD. Changes in the Chemical Components of Red and White Ginseng after Puffing. Korean J. Food Preserv. 2009;16(3):355-61.
  22. Oh JH, Choi BJ, Chang MS, Park SK. Nelumbo nucifera semen extract improves memory in rats with scopolamine-induced amnesia through the induction of choline acetyltransferase expression. Neurosci Lett. 2009;461(1):41-4. https://doi.org/10.1016/j.neulet.2009.05.045
  23. Hyman C, Hofer M, Barde YA, et al. BDNF is a neurotrophic factor for dopaminergic neurons of the substantia nigra. Nature. 1991;350(6315):230-2. https://doi.org/10.1038/350230a0
  24. Murer MG, Yan Q, Raisman-Vozari R. Brain-derived neurotrophic factor in the control human brain, and in Alzheimer's disease and Parkinson's disease. Prog Neurobiol. 2001;63(1):71-124. https://doi.org/10.1016/S0301-0082(00)00014-9
  25. Tsai SJ, Hong CJ, Liu HC, Liu TY, Hsu LE, Lin CH. Association analysis of brain-derived neurotrophic factor Val66Met polymorphisms with Alzheimer's disease and age of onset. Neuropsychobiology. 2004;49(1):10-2. https://doi.org/10.1159/000075332
  26. Petersen JD, Chen X, Vinade L, Dosemeci A, Lisman JE, Reese TS. Distribution of postsynaptic density (PSD)-95 and Ca2+/calmodulin-dependent protein kinase II at the PSD. J Neurosci. 2003;23(35):11270-8. https://doi.org/10.1523/jneurosci.23-35-11270.2003
  27. Wang SQ, Fang F, Xue ZG, Cang J, Zhang XG. Neonatal sevoflurane anesthesia induces long-term memory impairment and decreases hippocampal PSD-95 expression without neuronal loss. Eur Rev Med Pharmacol Sci. 2013;17(7):941-50.
  28. Sultana R, Banks WA, Butterfield DA. Decreased levels of PSD95 and two associated proteins and increased levels of BCl2 and caspase 3 in hippocampus from subjects with amnestic mild cognitive impairment: Insights into their potential roles for loss of synapses and memory, accumulation of Abeta, and neurodegeneration in a prodromal stage of Alzheimer's disease. J Neurosci Res. 2010;88(3):469-77. https://doi.org/10.1002/jnr.22227
  29. El-Husseini AE, Schnell E, Chetkovich DM, Nicoll RA, Bredt DS. PSD-95 involvement in maturation of excitatory synapses. Science. 2000;290(5495):1364-8. https://doi.org/10.1126/science.290.5495.1364
  30. Taft CE, Turrigiano GG. PSD-95 promotes the stabilization of young synaptic contacts. Philos Trans R Soc Lond B Biol Sci. 2013;369(1633):20130134. https://doi.org/10.1098/rstb.2013.0134
  31. Zhao H, Li Q, Zhang Z, Pei X, Wang J, Li Y. Long-term ginsenoside consumption prevents memory loss in aged SAMP8 mice by decreasing oxidative stress and up-regulating the plasticity-related proteins in hippocampus. Brain Res. 2009;1256:111-22. https://doi.org/10.1016/j.brainres.2008.12.031
  32. Jung TY, Jeong WC, Park JH. Therapeutic Potential of Jeongjihwan for the Prevention and Treatment of Amnesia. Journal of Physiology & Pathology in Korean Medicine. 2011;25(1):37-47.
  33. Han JS, Lee GS, Tak HS, Kim JS, Ra JW, Choi JE. Change of Neutral Ginsenoside Contents in Red and Fresh Ginseng (Panax ginseng C. A. Meyer) by Hydrolysis. Korean J. Medicinal Crop Sci. 2014;22(1):23-31. https://doi.org/10.7783/KJMCS.2014.22.1.23
  34. Choi JE, Nam KY, Li X, Kim BY, Cho HS, Hwang KB. Changes of Chemical Compositions and Ginsenoside Contents of Different Root Parts of Ginsengs with Processing Method. Korean J. Medicinal Crop Sci. 2010;18(2)118-25.
  35. Kim DH, Kwak KH, Lee KJ, Kim SJ, Shin YC, Chun BH, et al. Effects of Korea Red Ginseng Total Saponin on Repeated Unpredictable Stress-induced Changes of Proliferation of Neural Progenitor Cells and BDNF mRNA Expression in Adult Rat Hippocampus. J. Ginseng Res. 2004;28(4)94-103. https://doi.org/10.5142/JGR.2004.28.2.094
  36. Park EK, Han KH, Heo JH, Kim NK, Bae MH, Seo YH, et al. Effects of Ginseng Sprout extract and modified Kyung-Ok-Ko on scopolamine-induced cognitive impairment in mice. Journal of Agricultural, Life and Environmental Sciences. 2019;31(3):151-9.
  37. Jang SK, Ahn JW, Jo BR, Kim HS, Kim SJ, Sung EA, et al. Double-processed ginseng berry extracts enhance learning and memory in an Aβ42-induced Alzheimer's mouse model. KOREAN J. FOOD SCI. TECHNOL. 2019;51(2):160-8. https://doi.org/10.9721/KJFST.2019.51.2.160